2009 Vienna - European Society of Human Genetics

Cancer genetics
of the ZIC gene family, encodes a zinc finger transcription factor which
is important in neural crest proliferation and differentiation. We identified
ZIC2 as highly expressed gene in subset of colorectal tumors
using microarray validated by QPCR and immunohistochemistry. The
expression of ZIC2 was consistent with methylation status of gene promoter.
Here we show that ZIC2 interacts with β-catenin/TCF4-mediated
transcriptional activation of the β-catenin through binding to C terminal
domain of TCF4. The zinc finger domain of ZIC2 was found essential
for this interaction. By using animal cap study on Xenopus, we show
that Zic2 abrogates the effect of β-catenin on known Wnt target genes,
siamois and Xnr3. Moreover, ZIC2 represses the expression of Wnt
target genes cyclinD1 and c-Myc. Cell cycle analysis by flow cytometry
showed an increased fraction of cells in the G1 phase and subsequent
decrease in S phase in ZIC2 transfected cells. This is the first report of
a role for ZIC2 as a repressor of TCF4/B-catenin complex.
P06.103
cytogenetic studies on Breast cancer patients in tamilnadu,
india.
R. Sangeetha, V. Balachandar, P. Manikantan, S. Mohanadevi, K. Sasikala;
BHARATHIAR UNIVERSITY, COIMBATORE, India.
Breast cancer is the second most common cancer among women in
India. The focal aim of the present study has been indented to analyze
the chromosomal alterations and micronucleus in different stage of BC
patients in Tamilnadu population. The present study also aims to investigate
whether the CA15.3 level with BC patients exhibit increased CA
to address this issue. Furthermore, the most of samples were recruited
on the basis of family history and identify the genetic defects on BC
patients. In the present study 63 experimental subjects were selected
on the basis of CA15.3 marker which is the most widely used serum
biochemical tumor marker in breast cancer and equal number of controls
were selected and confirmed by CA53 level.
In the present study statistically significant results were obtained in
experimentals compared to controls. In the present study deletion
and translocation were frequently observed in chromosome 1, 11 and
17. (46, XX, del (1p - ); 46, XX, del (11q - ); 46, XX, del (17q - ). 46, XX, t
(1q +; 16q - ) ; 46, XX, t (11q +; 7p - ) ; 46, XX, t (17q +; 4q - )). Inversion and
satellite formation (21s + ) also frequently observed in experimentals. In
MN frequency also showed higher degree in experimentals compared
to controls (P