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440 11m. The amount of each amino acid was expressed as the molar percentage of the total
amino acids.
UV-induced UDS and survival assays
For UDS testing, MEFs (passage 4-6) were seeded onto coverslips. The next day, cells were
washed with PBS and irradiated at 16 11m 2 UV-C (Philips, TUV Jamp). Subsequently, cells
were incubated for 2.5 hours in culture medium containing 10 IlCi/ml eH]-thymidine, fixed
and subjected to autoradiography as described before (Vermeulen et al., 1994b).
For survival assays, MEF cultures were exposed to UV and allowed to grow for another 4-5
days, before reaching conflueney. Cells were labeled with ["'H]-thymidine as described above,
rinsed with PBS and lysed. The number of proliferating cells in each dish was estimated by
scintillation counting of the radioactivity during a 3 hours pulse-labeling. Cell survival was
expressed as the ratio of irradiated over un irradiated cells.
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