Declaration Dr. Thomas H. Pringle - Buffalo Field Campaign

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AY748671 ......................................-...................--............ hapB predicted V98 I60 ..... AY748672 ......................................-...................--............ hapB predicted V98 I60 ..... AY748679 ......................................-...................--............ hapB predicted V98 I60 ..... AY748617 .........T............................-.......T...........--............ hapB predicted V98 I60 ..... AY748699 ......................................-...................--............ hapB predicted V98 I60 ..... AY748620 ...........................T..........-...................--............ hapB predicted V98 I60 ..... AY748748 ......................................-...................--............ hapB predicted V98 I60 ..... AY748742 ......................................-...................--............ hapB predicted V98 I60 ..... AY748758 ......................................-...................--............ hapB predicted V98 I60 ..... AY748757 ......................................-...................--............ hapB predicted V98 I60 ..... AY748618 ......................................-...................--............ hapB predicted V98 I60 ..... AF083364 ......................................-...................--............ hapB predicted V98 I60 ..... AF083363 ......................................-...................--............ hapB predicted V98 I60 ..... AF083360 ......................................-...................--............ hapB predicted V98 I60 ..... AF083361 ......................................-...................--............ hapB predicted V98 I60 ..... U12941.. ......................................-...................--............ hapB predicted V98 I60 ..... U12943.. ......................................-...................--............ hapB predicted V98 I60 ..... U12960.. ......................................-...................--............ hapB predicted V98 I60 ..... GU947005 ......................................-...................--...........T hapC observed V98 I60 wHap15 AY748478 ......................................-...................--...........T hapC predicted V98 I60 ..... AY748476 ..A...................................-...................--...........T hapC predicted V98 I60 ..... AY748695 ..............................T.......-...................--...........T hapC predicted V98 I60 ..... AY748675 ......................................-...................--...........T hapC predicted V98 I60 ..... AY748673 ......................................-...................--...........T hapC predicted V98 I60 ..... AY748674 ......................................-...................--...........T hapC predicted V98 I60 ..... AY748529 ......................................-...................--...........T hapC predicted V98 I60 ..... AF083359 ......................................-...................--...........T hapC predicted V98 I60 ..... U12959.. ......................................-...................--...........T hapC predicted V98 I60 ..... U12942.. ......................................-...................--...........T hapC predicted V98 I60 ..... U12937.. ......................................-...................--...........T hapC predicted V98 I60 ..... U12951.. ......................................-...................--...........T hapC predicted V98 I60 ..... GU946999 ......................................-...................-....G.......T hapD observed V98 I60 bHap13 GU947002 ......................................-...................-....G.......T hapD observed V98 I60 bHap13 GU947003 ......................................-...................-....G.......T hapD observed V98 I60 bHap16 AY748682 ..........................................................--............ hapE weak pred V98 I60 ..... AY748669 ..........................................................--............ hapE weak pred V98 I60 ..... AY748521 ..........................................................--............ hapE weak pred V98 I60 ..... AF083358 ..........................................................--............ hapE weak pred V98 I60 ..... AY748681 ......................................-...................-............. hapF weak pred 98A 60N ..... U12864.. ......................................-...................-............. hapF weak pred 98A 60N ..... Table 9. Sequence data of Gardipee (22) for Yellowstone and Grand Tetons National Parks. First and second columns provide herd locations. The third column shows number of bison carrying the disease haplotype; the fourth the numbers of bison with wildtype mitochondrial DNA. The final column shows the percentage of healthy bison varies with geographic location of the herds (which have little observed mixing). Park Herd V98A I60N V98V I60I % Healthy YNP Hayden Valley 88 6 6% YNP Lamar Valley 19 22 54% YNP Mirror Plateau 10 6 38% GTNP Antelope Flats 20 0 0% GTNP Wolf Creek 8 0 0%
Figure 1. Structural models of the V98A region of bison cytochrome b adapted from previous crystallographic models of the bovine ortholog (57, 58). Here H97 is an axial ligand to heme iron and R100 forms a salt bridge to heme proprionate. " ,
Page 1 and 2: 02/14/2011 10:36 5206827080 Summer
Page 4 and 5: 02/14/2011 10:35 5205827080 " PICTU
Page 6 and 7: 02/14/2011 10:35 5205827080 PICTURE
Page 8 and 9: Below, analysis of complete bison m
Page 10 and 11: • The mutation rate in mitochondr
Page 12 and 13: possibility of mitochondrial dysfun
Page 14 and 15: arginine R100 provides an essential
Page 16 and 17: inbreeding, small herd sizes and ge
Page 18 and 19: 42. XB Q, Jianlin H et al. Assessme
Page 20 and 21: Table 3. Bison non-sporadic mitocho
Page 22 and 23: Table 6. A. Phylogenetic conservati
Page 26 and 27: DEVELOPMENT OF FECAL DNA SAMPLING M
Page 28 and 29: Table of Contents Abstract………
Page 30 and 31: List of Figures 1-1 Map of YNP show
Page 32 and 33: Dedication The work presented withi
Page 34 and 35: Preface Dale Lott grew up on the Na
Page 36 and 37: principles of the historic American
Page 38 and 39: within a possible third location (M
Page 40 and 41: samples. However, repeated genotypi
Page 42 and 43: population structure within YNP bre
Page 44 and 45: The highly differentiated populatio
Page 46 and 47: Figure 1-1. Map of YNP showing loca
Page 48 and 49: INTRODUCTION Wild bison are at risk
Page 50 and 51: sample was extracted twice using th
Page 52 and 53: Fragment analysis was carried out o
Page 54 and 55: visualized, assessed for quality, a
Page 56 and 57: aligned with less than two out of t
Page 58 and 59: ears (Ursus americanus), and brown
Page 60 and 61: Figures: Figure 2-1. Flow diagram s
Page 62 and 63: Table 2-2. Amplification success (A
Page 64 and 65: Appendix 2-3. Allelic dropout (AD)
Page 66 and 67: strong fine scale genetic different
Page 68 and 69: fidelity to breeding areas may be h
Page 70 and 71: RFLP PCR amplification was carried
Page 72 and 73: etween the Lamar Valley and Hayden
Page 74 and 75:
and Lamar Valley, in comparison to
Page 76 and 77:
The differences in haplotype freque
Page 78 and 79:
Figures: Figure 3-1. Approximate lo
Page 80 and 81:
Literature Cited Allendorf, F.W., R
Page 82 and 83:
Côté, S.D., F. Dallas, F. Marshal
Page 84 and 85:
Luikart, G., S. Zundel, D. Rioux, C
Page 86 and 87:
Tedford, S. Zimov, and A. Cooper. 2
Page 88 and 89:
general approach using mtDNA marker
Page 90 and 91:
2 K.C. Douglas et al. / Mitochondri
Page 93 and 94:
mitochondrial genome is 16318-16323
Page 95 and 96:
Bison Herd (bHap13 and bHap16), the
Page 97 and 98:
spread distribution of bison prior
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Declaration Dr. Thomas H. Pringle - Buffalo Field Campaign

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