Declaration Dr. Thomas H. Pringle - Buffalo Field Campaign

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Figures: Figure 2-1. Flow diagram showing the two extractions per fecal sample, and five, multiplex PCR amplification (using four microsatellite loci) per extraction, and number of genotypings per individual radio-collared bison used to assess amplification and genotyping error rates. Fecal Sample Extraction Extraction 1 Extraction 2 PCR 5 Genotypes 5 Genotypes 26
Tables: Table 2-1. Amplification success (AS), allelic dropout (AD) rates for all loci coamplified in the multiplex PCR. Total and average error rates were first estimated across all genotypings (loci and individuals), and then excluding data from two poor quality individual samples, YELL-024 and YELL-030. Average Extraction 1 Extraction 2 per locus Total Total errors Locus AS AD AS AD AS AD error rates w/o 024 & 030 BM711 0.93 0.11 0.95 0.21 0.94 0.16 0.16 0.03 BM2113 0.93 0.24 0.90 0.06 0.91 0.15 0.15 0.04 BMS1001 0.95 0.18 0.98 0.05 0.96 0.12 0.12 0.01 BMS2258 0.90 0.22 0.85 0.18 0.88 0.20 0.20 0.08 Averages: 0.93 0.19 0.92 0.12 0.92 0.16 0.14 0.04 Averages excluding YELL-024 & YELL-030: 0.97 0.21 0.97 0.04 0.97 0.12 27
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02/14/2011 10:36 5206827080 Summer
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02/14/2011 10:35 5205827080 " PICTU
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02/14/2011 10:35 5205827080 PICTURE
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Below, analysis of complete bison m
Page 10 and 11: • The mutation rate in mitochondr
Page 12 and 13: possibility of mitochondrial dysfun
Page 14 and 15: arginine R100 provides an essential
Page 16 and 17: inbreeding, small herd sizes and ge
Page 18 and 19: 42. XB Q, Jianlin H et al. Assessme
Page 20 and 21: Table 3. Bison non-sporadic mitocho
Page 22 and 23: Table 6. A. Phylogenetic conservati
Page 24 and 25: AY748671 ..........................
Page 26 and 27: DEVELOPMENT OF FECAL DNA SAMPLING M
Page 28 and 29: Table of Contents Abstract………
Page 30 and 31: List of Figures 1-1 Map of YNP show
Page 32 and 33: Dedication The work presented withi
Page 34 and 35: Preface Dale Lott grew up on the Na
Page 36 and 37: principles of the historic American
Page 38 and 39: within a possible third location (M
Page 40 and 41: samples. However, repeated genotypi
Page 42 and 43: population structure within YNP bre
Page 44 and 45: The highly differentiated populatio
Page 46 and 47: Figure 1-1. Map of YNP showing loca
Page 48 and 49: INTRODUCTION Wild bison are at risk
Page 50 and 51: sample was extracted twice using th
Page 52 and 53: Fragment analysis was carried out o
Page 54 and 55: visualized, assessed for quality, a
Page 56 and 57: aligned with less than two out of t
Page 58 and 59: ears (Ursus americanus), and brown
Page 62 and 63: Table 2-2. Amplification success (A
Page 64 and 65: Appendix 2-3. Allelic dropout (AD)
Page 66 and 67: strong fine scale genetic different
Page 68 and 69: fidelity to breeding areas may be h
Page 70 and 71: RFLP PCR amplification was carried
Page 72 and 73: etween the Lamar Valley and Hayden
Page 74 and 75: and Lamar Valley, in comparison to
Page 76 and 77: The differences in haplotype freque
Page 78 and 79: Figures: Figure 3-1. Approximate lo
Page 80 and 81: Literature Cited Allendorf, F.W., R
Page 82 and 83: Côté, S.D., F. Dallas, F. Marshal
Page 84 and 85: Luikart, G., S. Zundel, D. Rioux, C
Page 86 and 87: Tedford, S. Zimov, and A. Cooper. 2
Page 88 and 89: general approach using mtDNA marker
Page 90 and 91: 2 K.C. Douglas et al. / Mitochondri
Page 93 and 94: mitochondrial genome is 16318-16323
Page 95 and 96: Bison Herd (bHap13 and bHap16), the
Page 97 and 98: spread distribution of bison prior
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Declaration Dr. Thomas H. Pringle - Buffalo Field Campaign

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