Declaration Dr. Thomas H. Pringle - Buffalo Field Campaign
Declaration Dr. Thomas H. Pringle - Buffalo Field Campaign
Declaration Dr. Thomas H. Pringle - Buffalo Field Campaign
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
02/14/2011 10:36 5206827080 PICTURE ROCKS HDWR<br />
of interest (cytochrome b and A TP6). However, the variety of<br />
controls and re-analysis described in my paper strongly indicate that<br />
the Gardipee data can be unambiguously mapped into disease or notdisease<br />
ha.plotype and these shorter sequences can thus serve as a<br />
reliable proxy for full data, as they routinely are throughout<br />
biomedical genetics. The Ga.rdipee thesis is attached to my<br />
declaration.<br />
13. Below Ire-cast Gardipee's Table 3-1 into disease (hap6) and nondisease<br />
(hap8) coIwnns under the assumption that truncated microhaplotypes<br />
still provide a valid window into the status of the protein<br />
coding genes cytochrome b and A TP6.<br />
PAGE 05/06<br />
V98A I60N V98V 1601<br />
hap 6 hap 8<br />
Park Herd<br />
Hayden<br />
(deleterious) (healthy) % Healthy<br />
YNP<br />
YNP<br />
Valley<br />
Lamar Valley<br />
88<br />
19<br />
6<br />
22<br />
6%<br />
54%<br />
YNP Mirror Plateau 10 6 38%<br />
GTNP Antelope Flats 20 0<br />
0%<br />
GlNP WolfCreek 8<br />
°<br />
0%<br />
14. In some years the cull size has exceeded 1,000 animals and in one<br />
year the cull was approximately 1,400 individuals which may<br />
significantly exceed the total number of estimated hap 8 bison in the<br />
park (some 778 animals. assuming averaged YNP data above is<br />
roughly representative of current Park bison with total herd size taken<br />
as 3,500). However Park hison move about and herd numbers<br />
fluctuate; direct DNA testing of coralled animals prior to cull would<br />
be a more accurate way of measuring non-disease animals slated for<br />
cull.<br />
15. Because the hap 8 (healthy) bison are disproportionally in the<br />
Northern herd of the Park according to the best available data<br />
(Gardipee), they may be over-represented in corralled animals near<br />
Gardiner destined for slaughter, disproportionally reducing the<br />
number of hap 8 animals. These data suggest 2011 culls could worsen