Declaration Dr. Thomas H. Pringle - Buffalo Field Campaign

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Figures: Figure 3-1. Approximate locations of GYA bison breeding groups, represented by the haplotype frequencies of bison sampled within each area. Hayden 2006 Hayden 2005 Antelope Flats Hap 6 Hap 8 Lamar Valley Mirror Plateau Wolf Creek Road 44
Tables: Table 3-1. Distribution and frequency of haplotype 6 among the GYA bison populations sampled over two consecutive breeding seasons, 2005 and 2006, and the total number of samples analyzed from each population per season. N Haplotype 6 Park Breeding Group 2005 2006 Total 2005 2006 YNP Hayden 59 35 94 0.898 1.000 Lamar – 41 41 – 0.463 Mirror Plateau – 16 16 – 0.625 Total: 59 92 151 0.898 0.696 GTNP Antelope Flats – 20 50 – 1.000 Wolf Creek Road – 8 8 – 1.000 Total: 0 28 58 – 1.000 Table 3-2. FST values based on haplotype frequencies among GYA bison populations. LV HV-05 HV-06 MP YNP Lamar Valley (LV) – Hayden Valley (HV-05) 0.218** – Hayden Valley (HV-06) 0.367** 0.054* – Mirror Plateau (MP) 0.026 0.103** 0.231** – – GTNP 0.367** 0.054* 0.000 0.231** 0.191** *p = < 0.05, ** p = < 0.001 45
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02/14/2011 10:36 5206827080 Summer
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02/14/2011 10:35 5205827080 " PICTU
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02/14/2011 10:35 5205827080 PICTURE
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Below, analysis of complete bison m
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• The mutation rate in mitochondr
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possibility of mitochondrial dysfun
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arginine R100 provides an essential
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inbreeding, small herd sizes and ge
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42. XB Q, Jianlin H et al. Assessme
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Table 3. Bison non-sporadic mitocho
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Table 6. A. Phylogenetic conservati
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AY748671 ..........................
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DEVELOPMENT OF FECAL DNA SAMPLING M
Page 28 and 29: Table of Contents Abstract………
Page 30 and 31: List of Figures 1-1 Map of YNP show
Page 32 and 33: Dedication The work presented withi
Page 34 and 35: Preface Dale Lott grew up on the Na
Page 36 and 37: principles of the historic American
Page 38 and 39: within a possible third location (M
Page 40 and 41: samples. However, repeated genotypi
Page 42 and 43: population structure within YNP bre
Page 44 and 45: The highly differentiated populatio
Page 46 and 47: Figure 1-1. Map of YNP showing loca
Page 48 and 49: INTRODUCTION Wild bison are at risk
Page 50 and 51: sample was extracted twice using th
Page 52 and 53: Fragment analysis was carried out o
Page 54 and 55: visualized, assessed for quality, a
Page 56 and 57: aligned with less than two out of t
Page 58 and 59: ears (Ursus americanus), and brown
Page 60 and 61: Figures: Figure 2-1. Flow diagram s
Page 62 and 63: Table 2-2. Amplification success (A
Page 64 and 65: Appendix 2-3. Allelic dropout (AD)
Page 66 and 67: strong fine scale genetic different
Page 68 and 69: fidelity to breeding areas may be h
Page 70 and 71: RFLP PCR amplification was carried
Page 72 and 73: etween the Lamar Valley and Hayden
Page 74 and 75: and Lamar Valley, in comparison to
Page 76 and 77: The differences in haplotype freque
Page 80 and 81: Literature Cited Allendorf, F.W., R
Page 82 and 83: Côté, S.D., F. Dallas, F. Marshal
Page 84 and 85: Luikart, G., S. Zundel, D. Rioux, C
Page 86 and 87: Tedford, S. Zimov, and A. Cooper. 2
Page 88 and 89: general approach using mtDNA marker
Page 90 and 91: 2 K.C. Douglas et al. / Mitochondri
Page 93 and 94: mitochondrial genome is 16318-16323
Page 95 and 96: Bison Herd (bHap13 and bHap16), the
Page 97 and 98: spread distribution of bison prior
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