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APPROACH
MATERIALS AND METHODS
l. In Vivo studies
I.a. Animal treatment
I.b. Hemodynamic assessment
I.c. Collection of tissues
I.d.'Western Blot analysis on whole heart samples
I.e. Affymetrix gene-chip probe array analyses
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7T
IL In Vitro Studies
II.a. Adult cardiac myocytes isolation
II.b. Cell treatment
II.c. Western blot analysis
II.d. Arurexin-propidium iodide assay
ILe. Oxidative stress studies
7l
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III. Protein Assessment and Statistical Analysis
RESULTS
I. In Vivo Studies
La. General observations
Lb. Hemodynamic studies
Lc. Retinoic acid receptors
I.d. Bax and Bcl-xl expression in the hearts of treated animals
I.e. PPAR-ô receptor expression in hearts of treated animals
I.f. Afffrnetrix gene-chip probe anay analyses of the hearts
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IL In Vítro Studies
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1V