chapter 3 - RiuNet

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IN-DEPTH STUDY OF SPERM ACTIVATION IN PUFFERFISH 2.6 Statistical analysis The mean and standard error were calculated for all sperm quality parameters. Shapiro-Wilk and Levene tests were used to check the normality of data distribution and variance homogeneity, respectively. Oneway analysis of variance (ANOVA) and the Student's t-test were used to analyze data with normal distribution. Significant differences were detected using the Tukey multiple range test (P
CHAPTER 4 TM (%) 100 A a a Und-PD a Und-Epp 80 Dil-PD Dil-PD + BSA 60 40 20 b* c* b* b* * * 60 B a a a PM (%) 40 20 b* b* b* * * VCL (m/s) 250 200 150 100 50 C a b* b* c* a b* a * * * VSL (m/s) 200 150 100 50 D a b* b* c* a b* a * * * VAP (m/s) 250 200 150 100 50 E a b* b* c* a b* a * * * Control 1 2 4 7 11 15 Storage time (days) Figure 1. Evolution of sperm quality parameters in the different tested storageways compared with control samples after different incubation times (Und-PD, undiluted sperm stored in Petri dishes; Und-Epp; undiluted sperm stored in Eppendorf´s microtubes; Di-PD, diluted sperm stored in Petri dishes and Di- PD+BSA, diluted sperm containing 2% BSA stored in Petri dishes. Data are expressed as mean ± SEM (n=10). Different letters mean significant differences between storage-way at the same incubation time and the asterisk indicates significant differences with control samples. 92
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SPERM PHYSIOLOGY AND QUALITY IN TWO
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ACKNOWLEDGEMENTS Muchos años han p
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aunque tiene una alcachofa en la ca
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TABLE OF CONTENTS SUMMARY 1 RESUMEN
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SUMMARY SUMMARY The conservation st
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RESUMEN RESUMEN Las especies objeto
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RESUM RESUM Les espècies objecte d
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GENERAL INTRODUCTION 7
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GENERAL INTRODUCTION who postulated
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GENERAL INTRODUCTION 2. Sperm physi
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GENERAL INTRODUCTION Finally, the a
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GENERAL INTRODUCTION 3. Sperm quali
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GENERAL INTRODUCTION in fish (Kime
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GENERAL INTRODUCTION However, for s
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GENERAL INTRODUCTION future applica
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GENERAL INTRODUCTION With regards t
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OBJECTIVES The chapters presented b
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STANDARDIZATION OF SPERM MOTILITY E
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Page 50 and 51: STANDARDIZATION OF SPERM MOTILITY E
Page 56: CHAPTER 2 Study of the effects of t
Page 59 and 60: CHAPTER 2 reducing the pressure on
Page 61 and 62: CHAPTER 2 ongoing task in order to
Page 63 and 64: CHAPTER 2 To measure sperm density,
Page 65 and 66: CHAPTER 2 Spermiating males (%) 100
Page 67 and 68: CHAPTER 2 100 80 60 I II III IV A 4
Page 69 and 70: CHAPTER 2 3.2 Hormonal treatments P
Page 71 and 72: CHAPTER 2 With regard to the sperm
Page 73 and 74: CHAPTER 2 Relative volume (%) 80 I
Page 75 and 76: CHAPTER 2 In this respect, it is we
Page 77 and 78: CHAPTER 2 treatment to that of the
Page 79 and 80: CHAPTER 2 Our results demonstrate t
Page 82 and 83: ROLE OF IONS IN THE SPERM ACTIVATIO
Page 92: CHAPTER 4 Study of pufferfish (Taki
Page 95 and 96: CHAPTER 4 2002) so Takifugu niphobl
Page 97 and 98: CHAPTER 4 carrying out sperm collec
Page 99: CHAPTER 4 Table 1. Activation media
Page 103 and 104: CHAPTER 4 TM (%) 80 60 40 20 0 A a
Page 105 and 106: CHAPTER 4 TM (%) 80 A ASW100 GLU 60
Page 107 and 108: CHAPTER 4 although the activation m
Page 109 and 110: CHAPTER 4 dilution ratio and an opt
Page 111 and 112: CHAPTER 4 2006). Several studies in
Page 113 and 114: CHAPTER 4 5. Conclusions Some concl
Page 116 and 117: RELATIONSHIP BETWEEN SPERM MOTILIY
Page 130: RELATIONSHIP BETWEEN SPERM MOTILIY
Page 134 and 135: GENERAL DISCUSSION 1. Improvements
Page 136 and 137: GENERAL DISCUSSION profiles induced
Page 138 and 139: GENERAL DISCUSSION more studies on
Page 140 and 141: GENERAL DISCUSSION sperm motility p
Page 142: CONCLUSIONS 133
Page 145 and 146: CONCLUSIONS vii. The coefficients o
Page 148 and 149: REFERENCES Aarestrup K, Økland F,
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REFERENCES Beirão J, Cabrita E, P
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REFERENCES Cabrita E, Robles V, Cu
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REFERENCES De Vos A, Van De Velde H
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REFERENCES Gallego V, Pérez L, Ast
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REFERENCES sperm competition and fe
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REFERENCES Liu QH, Li J, Xiao ZZ, D
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REFERENCES Mortimer ST. Effect of i
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REFERENCES Peñaranda DS, Marco-Jim
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REFERENCES Rurangwa E, Volckaert FA
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REFERENCES Tesch F. Telemetric obse
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REFERENCES Wong PYD, Lee WM, Tsang
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