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EFFECT OF THERMAL REGIMES AND HORMONAL TREATMENTS The highest density values were observed in T20 males, with significant differences in most of the weeks (Fig. 2C). Similar density patterns were observed in T20 and T15 males, while T10 males showed an increase in their first 3 weeks of spermiation (10 th to 12 th week) followed by a marked decrease in the last week of the experiment (13th week). Regarding total and progressive motility (Fig. 2D and E), T20 treatment demonstrated the highest values, reaching maximum values of 75 and 35%, respectively at week 11. From this week to the end of treatment, T20 males displayed a marked decrease in total and progressive motility. Males subjected to the T10 thermal regime showed a significant but delayed increase in motility parameters from its first spermiation week (week 10) to the end of the experiment, and ended up exceeding the values obtained with the T15 and T20 treatments due to the fact that this coincided with the final reduction of motility in these treatments in the last week of treatment. With regard to the sperm motility classes, it was observed that the T10 and T20 treatments displayed better volume profiles (with relative volumes of maximum quality sperm (class IV) of 60% and 70%, respectively) than the T15 treatment, which showed values of around 50% in this same motility class (Fig. 3). In addition, the T20 treatment resulted in a higher number of weeks (7) with good quality samples, compared to either the T10 or T15 treatment, which only induced these good sperm samples for 3 and 5 weeks, respectively (Fig. 4). Relative volume (%) 80 I II 60 III IV 40 20 T10 T15 T20 Thermal treatments Figure 3. Percentage of total volume for each motility class (I–IV) in thermal treatments. I=no motile cells; II ≤25%; III=25–50% and IV>50% of motile cells. 57
CHAPTER 2 100 80 60 I II III IV A 40 20 0 Percentage of males (%) 100 80 60 40 20 0 B 100 80 C 60 40 20 0 5 6 7 8 9 10 11 12 13 Weeks of treatment Figure 4. Percentage of spermiating males from each motility class (I–IV) in each week throughout the thermal treatments: A) T10; B) T15 and C) T20. Class I=no motile cells; II≤25%; III=25–50% and IV>50% of motile cells. The kinetic parameters (Table 1) of the sperm cells showed a similar evolution in all thermal treatments, increasing as the weeks advanced. T20 induced the highest values in the three velocity parameters (VCL, VSL and VAP), with significant differences in several weeks. 58
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SPERM PHYSIOLOGY AND QUALITY IN TWO
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ACKNOWLEDGEMENTS Muchos años han p
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aunque tiene una alcachofa en la ca
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TABLE OF CONTENTS SUMMARY 1 RESUMEN
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SUMMARY SUMMARY The conservation st
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RESUMEN RESUMEN Las especies objeto
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RESUM RESUM Les espècies objecte d
Page 16: GENERAL INTRODUCTION 7
Page 19 and 20: GENERAL INTRODUCTION who postulated
Page 21 and 22: GENERAL INTRODUCTION 2. Sperm physi
Page 23 and 24: GENERAL INTRODUCTION Finally, the a
Page 25 and 26: GENERAL INTRODUCTION 3. Sperm quali
Page 27 and 28: GENERAL INTRODUCTION in fish (Kime
Page 29 and 30: GENERAL INTRODUCTION However, for s
Page 31 and 32: GENERAL INTRODUCTION future applica
Page 33 and 34: GENERAL INTRODUCTION With regards t
Page 36: OBJECTIVES The chapters presented b
Page 40 and 41: STANDARDIZATION OF SPERM MOTILITY E
Page 56: CHAPTER 2 Study of the effects of t
Page 59 and 60: CHAPTER 2 reducing the pressure on
Page 61 and 62: CHAPTER 2 ongoing task in order to
Page 63 and 64: CHAPTER 2 To measure sperm density,
Page 65: CHAPTER 2 Spermiating males (%) 100
Page 69 and 70: CHAPTER 2 3.2 Hormonal treatments P
Page 71 and 72: CHAPTER 2 With regard to the sperm
Page 73 and 74: CHAPTER 2 Relative volume (%) 80 I
Page 75 and 76: CHAPTER 2 In this respect, it is we
Page 77 and 78: CHAPTER 2 treatment to that of the
Page 79 and 80: CHAPTER 2 Our results demonstrate t
Page 82 and 83: ROLE OF IONS IN THE SPERM ACTIVATIO
Page 92: CHAPTER 4 Study of pufferfish (Taki
Page 95 and 96: CHAPTER 4 2002) so Takifugu niphobl
Page 97 and 98: CHAPTER 4 carrying out sperm collec
Page 99 and 100: CHAPTER 4 Table 1. Activation media
Page 101 and 102: CHAPTER 4 TM (%) 100 A a a Und-PD a
Page 103 and 104: CHAPTER 4 TM (%) 80 60 40 20 0 A a
Page 105 and 106: CHAPTER 4 TM (%) 80 A ASW100 GLU 60
Page 107 and 108: CHAPTER 4 although the activation m
Page 109 and 110: CHAPTER 4 dilution ratio and an opt
Page 111 and 112: CHAPTER 4 2006). Several studies in
Page 113 and 114: CHAPTER 4 5. Conclusions Some concl
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RELATIONSHIP BETWEEN SPERM MOTILIY
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GENERAL DISCUSSION 1. Improvements
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GENERAL DISCUSSION profiles induced
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GENERAL DISCUSSION more studies on
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GENERAL DISCUSSION sperm motility p
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CONCLUSIONS 133
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CONCLUSIONS vii. The coefficients o
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REFERENCES Aarestrup K, Økland F,
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REFERENCES Beirão J, Cabrita E, P
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REFERENCES Cabrita E, Robles V, Cu
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REFERENCES De Vos A, Van De Velde H
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REFERENCES Gallego V, Pérez L, Ast
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REFERENCES sperm competition and fe
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REFERENCES Liu QH, Li J, Xiao ZZ, D
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REFERENCES Mortimer ST. Effect of i
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REFERENCES Peñaranda DS, Marco-Jim
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REFERENCES Rurangwa E, Volckaert FA
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REFERENCES Tesch F. Telemetric obse
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REFERENCES Wong PYD, Lee WM, Tsang
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