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REFERENCES Liu QH, Li J, Xiao ZZ, Ding FH, Yu DD, Xu XZ. Use of computer-assisted sperm analysis (CASA) to evaluate the quality of cryopreserved sperm in red seabream (Pagrus major). Aquaculture 2007, 263(1-4):20-25. Mañanós E, Duncan N, Mylonas CC. Reproduction and control of ovulation, spermiation and spawning in cultured fish. In: Methods in reproductive aquaculture: Marine and freshwater species. Eds: Cabrita E, Robles V, Herráez MP. Taylor and Francis group, London, 2008; pp. 03-80 Marco-Jiménez F, Pérez L, Castro MPVD, Garzón DL, Peñaranda DS, Vicente JS, Jover M, Asturiano JF. Morphometry characterisation of European eel spermatozoa with computer-assisted spermatozoa analysis and scanning electron microscopy. Theriogenology 2006, 65(7):1302-1310. Marco-Jiménez F, Peñaranda DS, Pérez L, Viudes-De-Castro MP, Mylonas CC, Jover M, Asturiano JF. Morphometric characterization of sharpsnout sea bream (Diplodus puntazzo) and gilthead sea bream (Sparus aurata) spermatozoa using computer-assisted spermatozoa analysis (ASMA). J. Appl. Ichthyol. 2008, 24(4):382-385. Márián T, Krasznai Z, Balkay L, Emri M, Trón L. Role of extracellular and intracellular pH in carp sperm motility and modifications by hyperosmosis of regulation fo the Na + /H + exchanger. Cytometry 1997, 27(4):374-382. Martínez-Páramo S, Diogo P, Beirão J, Dinis MT, Cabrita E. Sperm lipid peroxidation is correlated with differences in sperm quality during the reproductive season in precocious European sea bass (Dicentrarchus labrax) males. Aquaculture 2012, 358–359(0):246-252. Martinez-Pastor F, Cabrita E, Soares F, Anel L, Dinis MT. Multivariate cluster analysis to study motility activation of Solea senegalensis spermatozoa: a model for marine teleosts. Reproduction 2008, 135:449–459. Martínez-Pastor F, Tizado EJ, Garde JJ, Anel L, de Paz P. Statistical series: Opportunities and challenges of sperm motility subpopulation analysis. Theriogenology 2011, 75(5):783-795. 151
REFERENCES Miller RL. Sperm chemo-orientation in the metazoa. In: Biology of Fertilization. Eds: Metz CB, Monroy A. Academic Press, New York, 1985; pp. 275-337. Miranda LA, Cassará MC, Somoza GM. Increase in milt production by hormonal treatment in the pejerrey fish Odontesthes bonariensis. Aquac. Res. 2005, 36(15):1473-1479. Miura T, Yamauchi K, Takahashi H, Nagahama Y. The role of hormones in the acquisition of sperm motility in salmonid fish. J. Exp. Zool. 1992, 261(3):359-363. Moccia RD, Munkittrick KR. Relationship between the fertilization of rainbow trout (Salmo gairdneri) eggs and the motility of spermatozoa. Theriogenology 1987, 27(4):679-688. Montano GA, Kraemer DC, Love CC, Robeck TR, O'Brien JK. Evaluation of motility, membrane status and DNA integrity of frozen–thawed bottlenose dolphin (Tursiops truncatus) spermatozoa after sex-sorting and recryopreservation. Reproduction 2012, 143(6):799-813. Morisawa M, Suzuki K. Osmolality and potassium ion: Their roles in initiation of sperm motility in teleosts. Science 1980, 210(4474):1145- 1147. Morisawa M. Adaptation and strategy for fertilization in the sperm of teleost fish. J. Appl. Ichthyol. 2008, 24(4):362-370. Morisawa S, Morisawa M. Acquisition of potential for sperm motility in rainbow trout and chum salmon. J. Exp. Biol. 1986, 126:89-96. Morisawa S. Ultrastructural studies of late-stage spermatids and mature spermatozoa of the puffer fish, Takifugu niphobles (tetraodontiformes) and the effects of osmolality on spermatozoan structure. Tissue and Cell 2001, 33(1):78-85. Mortimer S, Mortimer D, Fraser L. Guidelines on the application of CASA technology in the analysis of spermatozoa. Hum. Reprod. 1998, 13(1):142-145. 152
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SPERM PHYSIOLOGY AND QUALITY IN TWO
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ACKNOWLEDGEMENTS Muchos años han p
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aunque tiene una alcachofa en la ca
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TABLE OF CONTENTS SUMMARY 1 RESUMEN
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SUMMARY SUMMARY The conservation st
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RESUMEN RESUMEN Las especies objeto
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RESUM RESUM Les espècies objecte d
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GENERAL INTRODUCTION 7
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GENERAL INTRODUCTION who postulated
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GENERAL INTRODUCTION 2. Sperm physi
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GENERAL INTRODUCTION Finally, the a
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GENERAL INTRODUCTION 3. Sperm quali
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GENERAL INTRODUCTION in fish (Kime
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GENERAL INTRODUCTION However, for s
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GENERAL INTRODUCTION future applica
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GENERAL INTRODUCTION With regards t
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OBJECTIVES The chapters presented b
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STANDARDIZATION OF SPERM MOTILITY E
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CHAPTER 2 Study of the effects of t
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CHAPTER 2 reducing the pressure on
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CHAPTER 2 ongoing task in order to
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CHAPTER 2 To measure sperm density,
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CHAPTER 2 Spermiating males (%) 100
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CHAPTER 2 100 80 60 I II III IV A 4
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CHAPTER 2 3.2 Hormonal treatments P
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CHAPTER 2 With regard to the sperm
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CHAPTER 2 Relative volume (%) 80 I
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CHAPTER 2 In this respect, it is we
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CHAPTER 2 treatment to that of the
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CHAPTER 2 Our results demonstrate t
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ROLE OF IONS IN THE SPERM ACTIVATIO
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CHAPTER 4 Study of pufferfish (Taki
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CHAPTER 4 2002) so Takifugu niphobl
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CHAPTER 4 carrying out sperm collec
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CHAPTER 4 Table 1. Activation media
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CHAPTER 4 TM (%) 100 A a a Und-PD a
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CHAPTER 4 TM (%) 80 60 40 20 0 A a
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CHAPTER 4 TM (%) 80 A ASW100 GLU 60
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CHAPTER 4 although the activation m
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Page 113 and 114: CHAPTER 4 5. Conclusions Some concl
Page 116 and 117: RELATIONSHIP BETWEEN SPERM MOTILIY
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Page 134 and 135: GENERAL DISCUSSION 1. Improvements
Page 136 and 137: GENERAL DISCUSSION profiles induced
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Page 140 and 141: GENERAL DISCUSSION sperm motility p
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Page 145 and 146: CONCLUSIONS vii. The coefficients o
Page 148 and 149: REFERENCES Aarestrup K, Økland F,
Page 150 and 151: REFERENCES Beirão J, Cabrita E, P
Page 152 and 153: REFERENCES Cabrita E, Robles V, Cu
Page 154 and 155: REFERENCES De Vos A, Van De Velde H
Page 156 and 157: REFERENCES Gallego V, Pérez L, Ast
Page 158 and 159: REFERENCES sperm competition and fe
Page 162 and 163: REFERENCES Mortimer ST. Effect of i
Page 164 and 165: REFERENCES Peñaranda DS, Marco-Jim
Page 166 and 167: REFERENCES Rurangwa E, Volckaert FA
Page 168 and 169: REFERENCES Tesch F. Telemetric obse
Page 170 and 171: REFERENCES Wong PYD, Lee WM, Tsang
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