chapter 3 - RiuNet

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EFFECT OF THERMAL REGIMES AND HORMONAL TREATMENTS Table 1. Kinetic parameters (VCL, VSL and VAP) of sperm cells throughout the thermal treatments (T10, T15 and T20). Data are expressed as mean (SEM) and different letters indicate significant differences between treatments. VCL VSL VAP Week T10 T15 T20 T10 T15 T20 T10 T15 T20 5 65.5 (12.0) 25.5 (6.2) 39.6 (7.3) 6 127.8 (43.6) 95.9 (16.2) 61.0 (31.7) 39.0 (9.2) 83.0 (37.8) 56.1 (10.4) 7 24.1 (24.1) 77.2 (16.8) 5.4 (5.4) 27.6 (6.0) 12.1 (12.1) 43.8 (9.5) 8 82.2 (23.5) 140.0 (9.0) 36.3 (12.3) b 63.3 (6.2) a 48.6 (14.8) b 85.7 (5.9) a 9 85.6 (22.1) b 142.6 (9.0) a 39.9 (10.3) b 65.7 (5.3) a 53.7 (13.4) b 88.1 (5.9) a 10 33.5 (33.5) 117.7 (20.9) 126.0 (10.8) 6.7 (6.7) b 51.2 (14.4) ab 63.9 (6.0) a 15.7 (15.7) b 69.9 (15.8) ab 83.0 (7.1) a 11 122.1 (13.6) 122.7 (17.5) 149.9 (6.4) 49.1 (7.1) 57.5 (11.2) 72.2 (4.8) 69.8 (8.3) 76.0 (13.0) 94.3 (5.6) 12 119.2 (9.8) 123.1 (11.4) 121.0 (8.4) 57.8 (4.7) 52.8 (9.5) 57.0 (4.2) 77.0 (6.3) 71.5 (9.5) 73.5 (5.0) 13 133.2 (13.6) a 113.6 (12.4) ab 74.1 (5.1) b 56.4 (7.8) 49.1 (8.8) 30.2 (4.2) 79.7 (9.4) a 67.9 (9.4) ab 45.8 (3.8) b 59
CHAPTER 2 3.2 Hormonal treatments PMSG treatment induced lower percentages of spermiating males (with maximum values around 50–60%) than hCG and hCG rec treatments (Fig. 5A), both of which reached 100% in several weeks. In addition, PMSG males did not begin to spermiate until the 8 th week of treatment, while hCG and hCG rec males began spermiating earlier, in the 5 th week. An increasing trend in sperm volume (Fig. 5B) was displayed in all the hormonal treatments and hCG- and hCG rec -treated males showed higher values than PSMG-treated males, with significant differences in the 10 th , 11 th , 14 th and 18 th weeks. Maximum values for PMSG, hCG and hCG rec treatments were obtained in the 15 th , 19 th and 20 th weeks, respectively. Sperm density showed high variability under all the hormonal treatments (Fig. 5C). Samples from males treated with hCG rec demonstrated an increase in the first 5 weeks of treatment reaching maximum values of 18×10 9 , followed by a decrease from the 10 th week until the end of treatment. However, in the first 5 weeks of spermiation hCG-treated males displayed a progressive decrease and a gradual decline until the end of the treatment, reaching minimum values in the 19 th week. PMSG-treated males generated the highest values at the end of the treatment, with significant differences in the 15 th , 17 th , 18 th and 19 th weeks. With regard to motile and progressive motile cells (Fig. 5D and E), hCG rec treatment generated the highest values, reaching maximums of 70 and 35% (motile and progressive motile cells, respectively) in the 9 th week. Males treated with hCG showed a similar motility pattern to hCG rec males in the first weeks of treatment, but displayed a decrease in both parameters from the 11 th week to the end of treatment. PMSG-treated males showed an upward trend from the 11 th week, reaching motility and progressive motility values similar to hCG rec -treated males in the last 7 weeks of treatment. 60
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SPERM PHYSIOLOGY AND QUALITY IN TWO
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ACKNOWLEDGEMENTS Muchos años han p
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aunque tiene una alcachofa en la ca
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TABLE OF CONTENTS SUMMARY 1 RESUMEN
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SUMMARY SUMMARY The conservation st
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RESUMEN RESUMEN Las especies objeto
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RESUM RESUM Les espècies objecte d
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GENERAL INTRODUCTION 7
Page 19 and 20: GENERAL INTRODUCTION who postulated
Page 21 and 22: GENERAL INTRODUCTION 2. Sperm physi
Page 23 and 24: GENERAL INTRODUCTION Finally, the a
Page 25 and 26: GENERAL INTRODUCTION 3. Sperm quali
Page 27 and 28: GENERAL INTRODUCTION in fish (Kime
Page 29 and 30: GENERAL INTRODUCTION However, for s
Page 31 and 32: GENERAL INTRODUCTION future applica
Page 33 and 34: GENERAL INTRODUCTION With regards t
Page 36: OBJECTIVES The chapters presented b
Page 40 and 41: STANDARDIZATION OF SPERM MOTILITY E
Page 56: CHAPTER 2 Study of the effects of t
Page 59 and 60: CHAPTER 2 reducing the pressure on
Page 61 and 62: CHAPTER 2 ongoing task in order to
Page 63 and 64: CHAPTER 2 To measure sperm density,
Page 65 and 66: CHAPTER 2 Spermiating males (%) 100
Page 67: CHAPTER 2 100 80 60 I II III IV A 4
Page 71 and 72: CHAPTER 2 With regard to the sperm
Page 73 and 74: CHAPTER 2 Relative volume (%) 80 I
Page 75 and 76: CHAPTER 2 In this respect, it is we
Page 77 and 78: CHAPTER 2 treatment to that of the
Page 79 and 80: CHAPTER 2 Our results demonstrate t
Page 82 and 83: ROLE OF IONS IN THE SPERM ACTIVATIO
Page 92: CHAPTER 4 Study of pufferfish (Taki
Page 95 and 96: CHAPTER 4 2002) so Takifugu niphobl
Page 97 and 98: CHAPTER 4 carrying out sperm collec
Page 99 and 100: CHAPTER 4 Table 1. Activation media
Page 101 and 102: CHAPTER 4 TM (%) 100 A a a Und-PD a
Page 103 and 104: CHAPTER 4 TM (%) 80 60 40 20 0 A a
Page 105 and 106: CHAPTER 4 TM (%) 80 A ASW100 GLU 60
Page 107 and 108: CHAPTER 4 although the activation m
Page 109 and 110: CHAPTER 4 dilution ratio and an opt
Page 111 and 112: CHAPTER 4 2006). Several studies in
Page 113 and 114: CHAPTER 4 5. Conclusions Some concl
Page 116 and 117: RELATIONSHIP BETWEEN SPERM MOTILIY
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RELATIONSHIP BETWEEN SPERM MOTILIY
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GENERAL DISCUSSION 1. Improvements
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GENERAL DISCUSSION profiles induced
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GENERAL DISCUSSION more studies on
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GENERAL DISCUSSION sperm motility p
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CONCLUSIONS 133
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CONCLUSIONS vii. The coefficients o
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REFERENCES Aarestrup K, Økland F,
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REFERENCES Beirão J, Cabrita E, P
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REFERENCES Cabrita E, Robles V, Cu
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REFERENCES De Vos A, Van De Velde H
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REFERENCES Gallego V, Pérez L, Ast
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REFERENCES sperm competition and fe
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REFERENCES Liu QH, Li J, Xiao ZZ, D
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REFERENCES Mortimer ST. Effect of i
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REFERENCES Peñaranda DS, Marco-Jim
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REFERENCES Rurangwa E, Volckaert FA
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REFERENCES Tesch F. Telemetric obse
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REFERENCES Wong PYD, Lee WM, Tsang
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