chapter 3 - RiuNet

CHAPTER 1
On the contrary, the different magnification lenses used in this study
significantly affected some of the sperm quality parameters in the different
sperm classes (Table 2). Samples analyzed using the 20x lens showed lower
values than those analyzed using the 10x lens, with more significant
differences in C-II and C-III. In addition, the coefficients of variation within
the same motility class (Figure 1) with the 20x lens were much higher than
with the 10x lens, with much higher CV’s in C-I than in C-II and C-III.
Table 2. Mean ± SEM of sperm quality parameters for different magnification
lenses on different sperm classes (C-I, C-II, and C-III) at 30 s postactivation time.
C-I C-II C-III
10x 20x 10x 20x 10x 20x
MOT % 18.5 ± 1.8 18.3 ± 2.3 42.2 ± 3.1 39.1 ± 3.8 63.8 ± 2.1 56.6 ± 3.6
PM % 4.8 ± 0.9 5.0 ± 0.9 22.1 ± 2.3 18.6 ± 2.6 27.8 ± 2.0* 20.4 ± 2.3
FA % 8.4 ± 1.2 7.9 ± 1.3 32.0 ± 2.8 25.1 ± 3.2 47.8 ± 2.5* 36.1 ± 3.6
ME % 5.7 ± 0.7 4.6 ± 0.6 5.6 ± 0.6 7.3 ± 0.9 10.5 ± 0.8* 13.3 ± 1.0
SL % 4.4 ± 0.5 5.9 ± 0.9 4.6 ± 0.5 6.8 ± 1.0 5.5 ± 0.4* 7.1 ± 0.7
VCL µm/s 101.2 ± 4.6 89.2 ± 6.7 153.6 ± 5.1* 129.8 ± 8.6 152.3 ± 5.0* 129.4 ± 6.5
VSL µm/s 39.3 ± 2.8 36.2 ± 3.8 74.9 ± 4.1 64.6 ± 5.7 72.2 ± 3.8* 59.2 ± 4.2
VAP µm/s 58.3 ± 2.9 51.6 ± 4.3 94.6 ± 3.9 82.0 ± 5.9 95.4 ± 4.0* 81.9 ± 4.8
STR % 66.4 ± 1.6 67.4 ± 1.8 78.4 ± 1.4 76.4 ± 2.4 74.8 ± 1.1 71.3 ± 1.7
BFC beats/s 26.2 ± 1.2* 19.7 ± 2.6 35.7 ± 1.1* 31.1 ± 1.6 33.0 ± 1.1* 28.9 ± 1.2
Abbreviations: BCF, beat cross frequency; FA, fast; ME, medium; MOT, total
motility; PM, progressive motility; SL, slow; STR, straightness; VAP, average path
velocity; VCL, curvilinear velocity; VSL, straight line velocity.
3.2 Effect of frame rate setting
The frame rate setting (FR) had no effect neither on the total and
progressive motile cells nor on the proportion of fast, medium and slow
spermatozoa (data not shown). However, other kinetic values were deeply
affected by FR (Figure 2). VCL and BFC showed a progressive increase with
significant differences as the FR increased while STR showed a reverse
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