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CHAPTER 5 Relationship between spermatozoa motility parameters, sperm/egg ratio, and fertilization and hatching rates in pufferfish (Takifugu niphobles) V. Gallego a,b , L. Pérez a , J.F. Asturiano a and M. Yoshida b a Grupo de Acuicultura y Biodiversidad. Instituto de Ciencia y Tecnología Animal. Universitat Politècnica de València. Camino de Vera s/n. 46022, Valencia, Spain. b Misaki Marine Biological Station. Graduate School of Science. University of Tokyo. Miura, Kanagawa 238-0225, Japan. Aquaculture 2013; 416–417: 238-243 http://dx.doi.org/10.1016/j.aquaculture.2013.08.035 105
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SPERM PHYSIOLOGY AND QUALITY IN TWO
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ACKNOWLEDGEMENTS Muchos años han p
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aunque tiene una alcachofa en la ca
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TABLE OF CONTENTS SUMMARY 1 RESUMEN
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SUMMARY SUMMARY The conservation st
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RESUMEN RESUMEN Las especies objeto
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RESUM RESUM Les espècies objecte d
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GENERAL INTRODUCTION 7
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GENERAL INTRODUCTION who postulated
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GENERAL INTRODUCTION 2. Sperm physi
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GENERAL INTRODUCTION Finally, the a
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GENERAL INTRODUCTION 3. Sperm quali
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GENERAL INTRODUCTION in fish (Kime
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GENERAL INTRODUCTION However, for s
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GENERAL INTRODUCTION future applica
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GENERAL INTRODUCTION With regards t
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OBJECTIVES The chapters presented b
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STANDARDIZATION OF SPERM MOTILITY E
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CHAPTER 2 Study of the effects of t
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CHAPTER 2 reducing the pressure on
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CHAPTER 2 ongoing task in order to
Page 63 and 64: CHAPTER 2 To measure sperm density,
Page 65 and 66: CHAPTER 2 Spermiating males (%) 100
Page 67 and 68: CHAPTER 2 100 80 60 I II III IV A 4
Page 69 and 70: CHAPTER 2 3.2 Hormonal treatments P
Page 71 and 72: CHAPTER 2 With regard to the sperm
Page 73 and 74: CHAPTER 2 Relative volume (%) 80 I
Page 75 and 76: CHAPTER 2 In this respect, it is we
Page 77 and 78: CHAPTER 2 treatment to that of the
Page 79 and 80: CHAPTER 2 Our results demonstrate t
Page 82 and 83: ROLE OF IONS IN THE SPERM ACTIVATIO
Page 92: CHAPTER 4 Study of pufferfish (Taki
Page 95 and 96: CHAPTER 4 2002) so Takifugu niphobl
Page 97 and 98: CHAPTER 4 carrying out sperm collec
Page 99 and 100: CHAPTER 4 Table 1. Activation media
Page 101 and 102: CHAPTER 4 TM (%) 100 A a a Und-PD a
Page 103 and 104: CHAPTER 4 TM (%) 80 60 40 20 0 A a
Page 105 and 106: CHAPTER 4 TM (%) 80 A ASW100 GLU 60
Page 107 and 108: CHAPTER 4 although the activation m
Page 109 and 110: CHAPTER 4 dilution ratio and an opt
Page 111 and 112: CHAPTER 4 2006). Several studies in
Page 113: CHAPTER 4 5. Conclusions Some concl
Page 117 and 118: CHAPTER 5 Takifugu rubripes, is wid
Page 119 and 120: CHAPTER 5 immediately after its ext
Page 121 and 122: CHAPTER 5 Eggs from two females wer
Page 123 and 124: CHAPTER 5 The sperm was used to fer
Page 125 and 126: CHAPTER 5 100 80 r = 0.68 y = 1.40x
Page 127 and 128: CHAPTER 5 sperm samples (sperm acti
Page 129 and 130: CHAPTER 5 Similar data has been rep
Page 132: GENERAL DISCUSSION 123
Page 135 and 136: GENERAL DISCUSSION Chapter 1), the
Page 137 and 138: GENERAL DISCUSSION to obtain the fi
Page 139 and 140: GENERAL DISCUSSION Sperm motion par
Page 141 and 142: GENERAL DISCUSSION pufferfish sperm
Page 144 and 145: CONCLUSIONS i. The foundations of a
Page 146: REFERENCES 137
Page 149 and 150: REFERENCES development of media and
Page 151 and 152: REFERENCES Boryshpolets S, Kowalski
Page 153 and 154: REFERENCES Contri A, Valorz C, Faus
Page 155 and 156: REFERENCES Espinal J, Aldana M, Gue
Page 157 and 158: REFERENCES Iguer-ouada M, Verstegen
Page 159 and 160: REFERENCES Lecomte-Finiger R. Les s
Page 161 and 162: REFERENCES Miller RL. Sperm chemo-o
Page 163 and 164: REFERENCES Ohta, H., Unuma. Inducti
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REFERENCES European eel (Anguilla a
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REFERENCES Singh PB, Sahu V, Singh
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REFERENCES van Ginneken V, Dufour S
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REFERENCES Zilli L, Schiavone R, St
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