Cyanoguanidine CAS N°: 461-58-5

OECD SIDS
CYANOGUANIDINE
4. ECOTOXICITY ID: 461-58-5
DATE: 25.02.2004
REFERENCE SUBSTANCE: CuSO4-5H2O
DILUTION WATER
- Source: dechlorinated tap water
- Aeration: aerated sufficiently.
- Alkalinity: 29.0 mg/L
- Hardness: 40.5 mg/L as CaCO3
- Residual chlorine: less than 0.02 mg/L as Cl
- pH: 7.6
TEST SYSTEM
- Concentrations: 100 mg/L
- Renewal of test solution: 48 hr
- Exposure vessel type: size; 2.5 L test solution in a 3 L glass beaker
- Number of replicates, fish per replicate: 2, 5
- Test temperature: 24 plus or minus 1 degree
AERATION
- Dissolved oxygen: 6.7 - 8.3 mg/L
- pH: 7.2 - 7.7
- Intensity of irradiation: room light
- Photoperiod: 16 - 8hr light-dark cycle
DURATION OF THE TEST: 96 hr
TEST PARAMETER: mortality, abnormal behavior, abnormal respiration
SAMPLING: immediately after the preparation on 48 hour
MONITORING OF TEST SUBSTANCE CONCENTRATION: analyzed by
HPLC at the start and at the 48 hour exposure before renewal.
Test substance : SOURCE: Wako Pure Chemical Industries, LTD (Japan)
PURITY: 99.2%
IMPURITY/ADDITIVE/ETC.: not described
ANY OTHER INFORMATION: Lot No.WTM0467
Reliability : (1) valid without restriction
Flag : Critical study for SIDS endpoint
15.07.2003 (19)
Type : flow through
Species : Oryzias latipes (Fish, fresh water)
Exposure period : 14 day
Unit : mg/l
Analytical monitoring : yes
NOEC : m = 100
LC0 : m > 100
LC50 : m > 100
Method : OECD Guide-line 204 "Fish, Prolonged Toxicity Test: 14-day Study"
Year : 1998
GLP : yes
Test substance : other TS
Result : RESULTS:
- Measured concentrations:
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Nominal
Measured concentration [mg/L] (% of nominal)
concentration 0-day 7-day 14-day
[mg/L]
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Control