Cyanoguanidine CAS N°: 461-58-5

OECD SIDS
CYANOGUANIDINE
5. TOXICITY ID: 461-58-5
DATE: 25.02.2004
Plate: Two dish were used.
Application: Preincubation at 37 degree with shaking for 30 minutes.
Solvent: DMSO
Test substance : Chemiekombinat Bitterfeld, G. D. R.
Reliability : (3) invalid
26.11.2003 (56)
Type : Chromosomal aberration test
System of testing : Chinese hamster lung (CHL/IU) cells
Concentration : 24 and 48 hr Continuous treatment without metabolic activation: 210 - 840
ug/mL,
6 hr short-time treatment with or without metabolic activation: 210 - 840
ug/mL
The highest dose (840 ug/mL) was comparable to 10mmoL/L
Cycotoxic conc. : No growth inhibition was observed at any dose.
Metabolic activation : with and without
Result : negative
Method : OECD Guide-line 473 "Genetic Toxicology: In vitro Mammalian Cytogenetic
Test"
Year : 1998
GLP : yes
Test substance :
Remark : Solvent (solvent control): saline
Positive: Control:
24 and 48 hr Continuous treatment without metabolic activation: mitomycin
C 0.05, 0.025 ug/mL, respectively.
6 hr short-time treatment with or without metabolic cyclophophamide 12.5
ug/mL
Result : Genetoic effect:
No increase of structural or numerical aberration was observed at each
dose in all treatments with or without metabolic activation.
- 24 hr continuous treatment without metabolic activation: Negative,
- 48 hr continuous treatment without metabolic activation: Negative,
- 6 hr short-time treatment with metabolic activation: Negative,
- 6 hr short-time treatment without metabolic activation: Negative
Test condition : System of testing:
- Species/cell type: CHL/IU from NISH on Nov.1984
- Metabolic activation system: S9; Rat liver, induced with phenobarbitol and
5,6-benzoflavone
- No. of metaphases analyzed: 200 / dose (100 / dish)
Condition:
- Dose: All treatment; 210, 420, 840 (as 10mmol/L) ug/mL
- Number of replicates: 2
Pre-incubation time: 3 day
Criteria for Evaluating Results:
Negative; < 5%, Equivocal; 5% =< -