Cyanoguanidine CAS N°: 461-58-5
Cyanoguanidine CAS N°: 461-58-5
Cyanoguanidine CAS N°: 461-58-5
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OECD SIDS<br />
CYANOGUANIDINE<br />
5. TOXICITY ID: <strong>461</strong>-<strong>58</strong>-5<br />
DATE: 25.02.2004<br />
Result : TCLo = 2400 ug/m3<br />
Reliability : (3) invalid<br />
26.11.2003 (57)<br />
5.5 GENETIC TOXICITY ‘IN VITRO‘<br />
62<br />
Type : Ames test<br />
System of testing : Salmonella typhimurium TA98, TA100, TA1535, TA1537 and Escherichia<br />
coli WP2uvrA<br />
Concentration : 0, 156, 313, 625, 1250, 2500, 5000 ug/plate<br />
Cycotoxic conc. : No cytotoxicity was observed at any dose.<br />
Metabolic activation : with and without<br />
Result : negative<br />
Method : other: OECD Guide-line 471 and 472<br />
Year :<br />
GLP : yes<br />
Test substance : other TS<br />
Remark : Solvent (solvent control): DMSO<br />
Positive: Control:<br />
Without metabolic activation; 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide<br />
(TA100, TA98 and WP2uvrA), sodium azide (TA1535), 9-aminoacridine<br />
hydrochloride (TA1537)<br />
With metabolic activation; 2-aminoanthracene (each strain)<br />
Result : Genetoic effect:<br />
No increase of revertants was observed at each dose in all strains with or<br />
without metabolic activation.<br />
- With metabolic activation: Negative<br />
- Without metabolic activation: Negative<br />
Test condition : System of Testing<br />
- Metabolic activation system: S9 from rat liver, induced with phenobarbital<br />
and 5,6-benzoflavone<br />
Condition:<br />
- Number of replicates: 2<br />
- Plates per test: 3<br />
- Application: pre-incubation (37 degree, 20 minutes)<br />
- Positive and negative control groups and treatment:<br />
- Incubation time: 48 hr<br />
Criteria for Evaluating Results:<br />
(1) The revertant colony on this substance increase should be more than<br />
two times of the control.<br />
(2) The concentration dependency should be shown in the revertant colony<br />
increase.<br />
Test substance : Purity: 99.8% (impurities as melamine are included 0.01-0.02%).<br />
lot No. :L-2271,<br />
NIPPON CARBIDE INDUSTRIES CO.,INC.<br />
Reliability : (1) valid without restriction<br />
Flag : Critical study for SIDS endpoint<br />
26.11.2003 (39)<br />
Type : Ames test<br />
System of testing : Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538<br />
Concentration : 1, 2.5, 5, 10, 25, 50, 100, 150 ug/plate<br />
Cycotoxic conc. : No cytotoxicity was observed at any dose.<br />
Metabolic activation : with and without<br />
Result : positive<br />
Method : other<br />
Year : 1985<br />
GLP : yes<br />
Test substance : other TS<br />
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