Brugia Malayi - Clark Science Center - Smith College

Hsp25 expression was quantified by immunoblotting a 1D-gel with a polyclonal anti-Hsp25 antibody. Expression increased
from myoblasts to early myotubes, the point of initial fusion, and then decreased once the more stable late myotubes developed
(Figure 2). These data indicate that the cells may be recruiting Hsp25 during the stress of fusion, however more studies are needed
to confirm this correlation. The future trajectory is to establish the influence of Hsp25 on the entire proteome using an RNA
silencing technique to knock-down Hsp25 prior to fusion. (Supported by the Wilens Fund in the Biological Sciences)
Advisor: Stylianos Scordilis
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Time-point in C2C12 Myogensis
Figure 2: Hsp25 expression during C2C12 differentiation.
References:
Fulda, S., Gorman, A., Hori, O., and A. Samali, 2010, Cellular Stress Responses: Cell Survival and Cell Death. Intl J Cell Biol. Article ID 214074: 23 pages.
Morton, J., Kayani, A., McArdle, A., and B. Drust, 2009, The exercise-induced stress response of skeletal muscle, with specific emphasis on humans. Sports Med.
39(8):643-662.
Stokoe, D., Engel, K., Campbell, D., Cohen, P., and M. Gaestel, 1992, Identification of MAPKAP kinase 2 as a major enzyme responsible for the phosphorylation
of the small mammalian heat shock proteins. Fed Eur Biochem Soc. 313(3):307-313.
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