A guide for the preparation and use of buffers in biological systems

Tris Buffered Saline (TBS), pH 7.4
10 mM Tris
150 mM NaCl
(1 liter of TBS can be prepared by dissolving 1.21 g of Tris base and
8.7 g of NaCl in 1 liter of distilled water. Adjust the pH before use.
Note: Tris has a pK a
of 8.3. Hence, the buffering capacity at pH 7.4
is minimal compared to phosphate buffer (pK a
= 7.21).
TBST (Tris Buffered saline and TWEEN®-20)
10 mM Tris-HCl, pH 8.0
150 mM NaCl
0.1% TWEEN®-20
Stripping Buffer for Western Blotting Applications
62.5 mM Tris buffer, pH 6.7 to 6.8
2% Sodium dodecyl sulfate (SDS)
100 mM β-Mercaptoethanol
Cell Lysis Buffer
20 mM Tris-HCl (pH 7.5)
150 mM NaCl
1 mM Sodium EDTA
1 mM EGTA
1% TRITON® X-100
2.5 mM Sodium pyrophosphate
1 mM β-Glycerophosphate
1 mM Sodium orthovanadate
1µg/ml Leupeptin
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