Axiocam ERc 5s Operating Instructions - Carl Zeiss

AxioVision software
Software products
AxioVision (LE) is the free imaging software available from Carl Zeiss.
The program is installed in the manner described in the section “Installing
configuration program and AxioVision LE” on the “Operation with PC
connection and software” tab.
AxioVision (full version) is the standard software available from Carl Zeiss
for image acquisition, analysis, processing and archiving.
Acquiring your first image
The steps below describe the process for acquiring a simple image. You can
access the online help for each function using the F1 function key.
1 Start the AxioVision software (LE or full version).
2 If you have several cameras connected to the system, select the desired
camera in the Acquisition menu | Select Camera.
3 Switch the microscope’s light path to the phototube.
4 In order to display the camera’s live image, click on the Live button (or the
F3 key).
5 To adjust the camera and the live image display, click on the Properties
button. Enter the desired settings on the Display, Adjust, Frame and
General property pages.
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Set the exposure time: Adjust property page, Measure button
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Focus the camera image
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Select the frame
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Select the appropriate scaling for the objective: Pixel drop-down list
6 Acquire a single image using the Snap button.
7 To adjust the image subsequently, click on the Properties button and then
on the Display property page.
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Adjust the image individually: Brightness and Contrast sliders
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Set brightness and contrast automatically: Min/Max button
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Undo changes: Linear button
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Show scale bars in the image: Scale bar button
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Generating annotations: Draw in annotations button
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Adjust live image size: Navigator button
8 Save the image (ZVI image format) using the Save button or export the
image to an image format of your choice via the File menu | Export.
i
Note:
The AxioCam ERc 5s differs from other AxioCam cameras from Carl Zeiss on account
of a number of functions. For example, the AxioCam ERc 5s already works with a
color profile in the live image. This means that it is no longer necessary to perform
an adjustment to the gamma value of the monitor.
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Additional software modules
Amongst the wide range of AxioVision software modules (LE and full version),
the following are particularly useful for the AxioCam ERc 5s:
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Module
HDR Imaging
Interactive
Measurement
Description
Extends the camera’s dynamic range. By acquiring a series of
exposures and merging the individual images to form one image it is
possible to achieve finer brightness gradations in the resulting image
and a wider contrast range.
Collection of measurement tools for analyzing images directly within
AxioVision. The results are displayed in tabular form or can be
embedded in special report templates.
AxioVision software