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Axiocam ERc 5s Operating Instructions - Carl Zeiss

Axiocam ERc 5s Operating Instructions - Carl Zeiss

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AxioCam <strong>ERc</strong> <strong>5s</strong><br />

Microscope camera<br />

<strong>Operating</strong> instructions


Control elements, indicators and connections<br />

1<br />

2 3<br />

4<br />

5<br />

Front of camera:<br />

1 LED indicator<br />

2 Manual acquisition<br />

3 AWB: Automatic<br />

white/gray balance<br />

4 Receiver for IR remote<br />

control<br />

5 Card slot for SD/SDHC<br />

memory cards<br />

1<br />

2<br />

3<br />

Back of camera:<br />

1 USB port for data<br />

transfer and power<br />

supply<br />

2 DVI signal via HDMI<br />

port for monitor, TV<br />

or beamer<br />

3 S-Video connection for<br />

recorder or monitor<br />

Definitions<br />

SD memory card: Digital storage medium for recording image data and<br />

other information. On SD cards an amount of 4 GB of data can be stored;<br />

up to 32 GB on new SDHC cards (HC=high capacity). SD cards should be<br />

formatted on a PC to FAT32 format.<br />

C-mount adapter: The C-mount is a standardized mounting thread<br />

for camera lenses. In microscopy the C-mount is used to connect digital<br />

cameras. So it is guaranteed that the image produced by the microscope<br />

objective is large enough to illuminate the small camera sensors.<br />

AWB (Automatic White Balance): The purpose of the white balance is<br />

to calibrate the camera to the color temperature of the incident light and<br />

thereby prevent color cast. However, the automatic white balance that is<br />

normally used does not work, or does not work sufficiently well, in the case<br />

of technical surfaces or macros. This is because these objects often do not<br />

have any defined pure-white or color-neutral surfaces that the automatic<br />

white balance can take as a basis for the calibration. Instead it uses the<br />

average values of the individual red, green and blue (RGB) color channels<br />

for reference. It is therefore necessary to perform the white balance<br />

manually using defined color-neutral objects.<br />

Parfocality: According to Professor A. Köhler, the focus plane set should<br />

be retained when eyepieces or objectives are changed on the microscope.<br />

Ideally, the object should appear just as sharp as it did before the eyepiece<br />

or objective was changed. Today, this also applies to the phototube for the<br />

acquisition of images using a digital camera: the sharpness of the camera’s<br />

images should correspond to that of the image you see when you look<br />

through the eyepiece. It is possible to set the parfocality of the camera even<br />

with the help of a reticle in the intermediate image of the eyepiece or with<br />

the help of an adapted monitor.


Find what you need quickly with the Infoflip<br />

The Infoflip contains all the information and instructions you need to produce<br />

the best possible photos using the camera and imaging software.<br />

Further information can be found in the online help of the programs supplied<br />

(F1 key) and in additional documentation on the DVD provided. We also offer<br />

tips and tricks on the camera and programs on our Internet site.<br />

Who the Infoflip is for<br />

Ideally, you will have a good knowledge of microscope technology, educational<br />

and routine microscopy and image analysis. The camera should only be<br />

installed and operated by trained individuals who have been informed about<br />

the possible risks associated with microscopy and the area of application in<br />

question.<br />

Your sales partner will also be happy to install the camera and the software.<br />

Maintenance and repairs should only be carried out by trained personnel, e.g.<br />

your sales partner or qualified personnel authorized by <strong>Carl</strong> <strong>Zeiss</strong>.<br />

Before installing the camera, you must read the safety instructions on the<br />

flap and the Functional description / use tab, which contains essential<br />

information relating to the camera system.<br />

We wish you every success in your day-to-day work with the camera system!


Symbols used<br />

!<br />

i<br />

Danger!<br />

This symbol warns you about physical dangers! It is possible for injuries to be<br />

sustained, including fatal injuries. The instructions that follow this symbol must<br />

be observed!<br />

!<br />

Caution!<br />

In these situations damage may be caused to the microscope and camera. It is<br />

also possible that imperfect imaging results may be achieved. Please observe the<br />

instructions!<br />

!<br />

i<br />

Note:<br />

Here you will find tips and tricks to help you achieve the best results.<br />

For your safety<br />

Please ! observe all operating instructions and instructions for use relating to<br />

all devices that may be used in connection with the camera (microscopes,<br />

microscope accessories, PC, etc.). Also take any safety instructions and<br />

requirements that apply within your working environment into account.<br />

!<br />

Danger!<br />

Fatal injuries! <strong>Operating</strong> the camera in areas where there is an explosion risk,<br />

in the presence of volatile anesthetic agents or in the presence of flammable<br />

solvents, such as alcohol, gasoline or similar substances, can result in explosions<br />

or deflagrations. Do not operate the camera in such environments!<br />

Permanent damage to eyesight! The light beam at the uncovered<br />

microscope port can be so bright as to cause permanent damage to the retina if<br />

you look into it, which can lead to complete blindness. Please do not look into<br />

the microscope port under any circumstances while the microscope’s light source<br />

is switched on!<br />

Cuts and bruises! The edges of the camera housing and the corners are sharp.<br />

Handle the camera carefully to avoid injuring yourself! Avoid letting the camera<br />

fall onto unprotected feet! Warn colleagues in the vicinity of the attached<br />

camera against touching the housing!<br />

Death and serious injury due to electric shock! The mains power adapter<br />

works i with high voltage that can cause fatal injury. When using the mains power<br />

adapter for the camera, you must check regularly that the power adapter’s<br />

housing, its wall plug and the wall socket are not damaged. Handle the power<br />

adapter with care and never let it fall onto hard surfaces!<br />

!<br />

Caution!<br />

Damage and imperfect results with incompatible microscopes!<br />

Not all commercially available microscopes are suitable to be connected to<br />

the camera. Please check this by consulting with the manufacturer concerned!<br />

Always follow the instructions for the microscopes when it comes to connecting<br />

and operating external cameras.<br />

Damage ! due to short circuit! If the cable of the mains power adapter is<br />

damaged, this can lead to a short circuit or loose connection; the camera will<br />

then no longer function, or will no longer function reliably. Handle the power<br />

adapter’s cable with care!<br />

Damage due to defective connections! The standardized connectors<br />

and ports for establishing connections to the camera are very small and can be<br />

damaged if the connectors are inserted incorrectly. Check that the connector is<br />

correctly aligned with the port before you insert it! Never insert the connector<br />

forcefully or with a jolt! Never remove connectors by pulling on the cable –<br />

always pull the connector itself!<br />

Artifacts on photos due to dirt! Dust particles and fluff on the camera’s<br />

filter glass show up in photos as spots and black points. Always store the camera<br />

with the dust cap on. Make sure the environment is free of dust when you attach<br />

the camera. Never touch the filter glass with your bare hands.


Functional description / use<br />

Brief description of the camera system<br />

The AxioCam <strong>ERc</strong> <strong>5s</strong> CMOS camera is intended as a camera for connection to<br />

microscopes and produces photos with a size of up to 5 megapixels. The image<br />

data can be<br />

• saved to a memory card (stand-alone operation),<br />

• immediately viewed on a connected monitor, or<br />

• immediately displayed via the configuration program and processed further<br />

on a PC using the AxioVision programs (LE or full version).<br />

Various standard analog and digital data interfaces make it possible to connect<br />

the camera to different output devices for co-observation or recording.<br />

The camera quality is configured and optimized by means of settings in the<br />

configuration program supplied. This ensures that the settings are applied<br />

during stand-alone operation – without connection to a PC.<br />

Purpose<br />

The AxioCam <strong>ERc</strong> <strong>5s</strong> is a microscope camera that is suitable for use as an<br />

accessory for educational and routine microscopy in laboratory environments<br />

and for use by trained laboratory personnel.<br />

• The camera must be permanently connected to <strong>Carl</strong> <strong>Zeiss</strong> light microscopes<br />

or suitable light microscopes of other manufacturers by means of suitable<br />

adapters.<br />

• The camera must be connected to microscope ports intended for this<br />

purpose; it must not be connected to the eyepiece.<br />

• The camera is not suitable for fluorescence microscopy.<br />

• The camera is configured and controlled by means of the PC software<br />

supplied, which also allows the photos to be further processed and saved.<br />

• The camera can be connected to co-observation and recording devices<br />

by means of various standardized data interfaces. Only pre-fabricated,<br />

standard connection cables may be used.<br />

Obligations, assurances and exclusions<br />

The envisaged uses of the camera system are general tasks and applications in the fields<br />

of microscopy and image analysis.<br />

This Infoflip and other information on the camera system provide only a general<br />

description of technical procedures and instructions. It may not be appropriate to follow<br />

the instructions in each individual case. Please contact the <strong>Carl</strong> <strong>Zeiss</strong> support team if in<br />

doubt!<br />

All user information on the camera system and this Infoflip are protected by copyright.<br />

<strong>Carl</strong> <strong>Zeiss</strong> has reserved all rights to this information. The production of copies or partial<br />

copies and translation into other languages are also not permitted without specific<br />

written consent. Copies intended for personal use are excluded from this.<br />

<strong>Carl</strong> <strong>Zeiss</strong> would like to point out that the details contained in all the user information<br />

relating to the camera system are updated on an ongoing basis in line with any technical<br />

changes that may be necessary. This Infoflip therefore only reflects the technical status<br />

of the camera system at the time of going to print. You will find up-to-date information<br />

relating to the instructions and the camera system on our website!<br />

Before installing and operating the camera system, make sure you have read and<br />

understood all the information in this Infoflip and in the other accompanying documents!<br />

<strong>Carl</strong> <strong>Zeiss</strong> does not assume any liability or give a warranty for applications other than<br />

those described here. The same applies to procedures that are not described in this<br />

Infoflip and do not produce the desired result, or even result in damage.<br />

Functional description / use


Included with product<br />

Included in standard package<br />

• AxioCam <strong>ERc</strong> <strong>5s</strong> camera with dust cap<br />

• A to Mini B type USB cable<br />

1 Camera<br />

2 USB cable<br />

In addition:<br />

• DVD containing the configuration program and the AxioVision LE imaging<br />

software (upgrade to full version available as an option)<br />

• <strong>Operating</strong> instructions (this Infoflip)<br />

i<br />

Note:<br />

A C-mount adapter for the transition between the camera and microscope is not<br />

supplied! Order the adapter separately based on the required magnification and<br />

the necessary adaptation to your microscope (also applies to microscopes from<br />

third-party manufacturers). <strong>Carl</strong> <strong>Zeiss</strong> offers various camera adapter interfaces for<br />

its own microscope lines. The smaller the numerical value printed on these different<br />

adapters, the more of the image observed through the eyepiece is covered by the<br />

camera ! image. In all cases the frame visible in the photo will be smaller than the<br />

image observed through the eyepiece.<br />

!<br />

Included in stand-alone package (optional)<br />

This package can be ordered as an option in addition to the standard package.<br />

It is useful for situations when you want to operate the camera without a<br />

connection to a PC. In this case the image data are saved to a memory card.<br />

• Mains power supply with country-specific plug adapters<br />

• IR remote control to trigger the camera remotely<br />

• SDHC type memory card with a capacity of 4 GB, incl. protective case<br />

• Card reader for this memory card<br />

• HDMI to DVI video adapter to connect PC monitors or beamers<br />

i<br />

1 Power supply (with<br />

country-specific<br />

adapters)<br />

2 Remote control<br />

3 Memory card<br />

4 Card reader<br />

5 Video adapter<br />

!<br />

Caution!<br />

Imaging errors due to damage caused in transit! In spite of the padded outer<br />

packaging, it is possible that damage may be caused to the camera. Check that the<br />

outer packaging or camera packaging does not appear to be damaged! Report any<br />

obvious damage to the carrier and vendor.<br />

!<br />

Included with product


Specifications<br />

Camera<br />

Sensor Micron MT9P031, 5 megapixels (resolution 2560 x 1920),<br />

type: CMOS<br />

Sensor size 5.7 mm x 4.28 mm, equivalent: 1/2.5“, diagonal: 7.1 mm<br />

Spectral approx. 400-700 nm, IR blocking filter<br />

sensitivity<br />

Exposure time 10 µs to 2 s, max. 13 fps at 800 x 600 pixels<br />

Color resolution 3 x 8 bit<br />

Interfaces 1 x SD/SDHC slot, 1 x Mini USB 2.0,<br />

1 x AV (S-Video), 1 x DVI (HDMI)<br />

Optical interface C-mount, with corresponding adapters<br />

Power supply via USB<br />

Operation Dual color LED, red/green, flashing<br />

indicator<br />

Control Button for image acquisition, button for white balance<br />

elements<br />

Dimensions 65 mm x 65 mm x 63 mm (W x D x H)<br />

Weight 250 g<br />

Ambient<br />

conditions<br />

+5°C to +45°C, max. 80% relative humidity,<br />

no condensation, free air circulation necessary for cooling<br />

Stand-alone package (optional)<br />

Power adapter Connection: Mini USB 2.0<br />

Output: 5 V / 1 A<br />

Input: 100-240 VAC, 50-60 Hz, with country-specific<br />

plug adapters<br />

Remote control IR remote trigger<br />

Memory card SDHC type with a capacity of 4 GB, in protective case<br />

Card reader USB connection on PC side, suitable for SD and SDHC cards<br />

Video adapter HDMI to DVI<br />

Relative spectral sensitivity AxioCam <strong>ERc</strong> <strong>5s</strong> (incl. IR filter)<br />

Relative spectral sensitivity<br />

Wavelength (nm)


Installation of the camera<br />

Attaching the camera to the microscope<br />

Screwing the C-mount adapter onto the camera<br />

i<br />

Note:<br />

Select the C-mount adapter that offers the required scale of magnification and is<br />

suitable for your microscope. The C-mount adapter is not included with the camera<br />

and must be ordered from <strong>Carl</strong> <strong>Zeiss</strong>.<br />

!<br />

1 The room should be free of dust to ensure that<br />

the camera interior remains dust-free. Use a<br />

table with a padded surface that will prevent<br />

damage to the camera and C-mount adapter in<br />

the event that they are dropped.<br />

2 Remove the dust cap from the sensor opening.<br />

i<br />

3 ! Ensure the C-mount adapter is placed onto the<br />

camera thread straight so that the thread is not<br />

damaged when you screw it on.<br />

4 Carefully screw the threaded end of the C-mount adapter into the thread<br />

on the camera housing. Screw the adapter in hand-tight.<br />

!<br />

Screwing the camera with adapter onto<br />

the microscope<br />

!<br />

Danger!<br />

Permanent damage to eyesight! Switch off the microscope illumination!<br />

1 Remove any dust cap that may be present from the microscope’s<br />

phototube.<br />

2 Place the unit comprising the camera and C-mount adapter onto the<br />

microscope’s phototube.<br />

3 Align the camera by rotating it so that<br />

the front of the housing (where the<br />

model name is shown) is parallel to the<br />

front of the microscope, i.e. is pointing<br />

towards the eyepiece (see image<br />

on right).<br />

4 Fasten the camera:<br />

––<br />

In the case of adapters that are fastened<br />

to the interface using 3 set screws: using<br />

a 3 mm hex key (as supplied with ZEISS<br />

microscopes), carefully turn the screws<br />

hand-tight, one after the other, on the<br />

flange of the microscope (see image at<br />

bottom right).<br />

––<br />

In the case of adapters with outer thread:<br />

place camera onto interface, align it and<br />

fasten it to the microscope by turning the<br />

outer thread.<br />

5<br />

i<br />

When you acquire your first photos,<br />

check the correct alignment and<br />

sharpness of the camera via live image on an adapted monitor or PC.<br />

If necessary, repeat the procedure.<br />

!<br />

Caution!<br />

Filter holder on the camera can come loose, meaning parfocality is lost! Before<br />

correcting the alignment, you must loosen the adapter so that the camera can be<br />

turned easily.<br />

!<br />

Installation of the camera


Operation without PC connection<br />

<strong>Operating</strong> the camera without PC connection<br />

In this operating mode you trigger the acquisition of images manually using a control<br />

button on the camera or by means of the optional remote control. The camera then saves<br />

the image data to an SD card in the card slot on the camera. For this you need:<br />

• SD memory card<br />

• Mains power adaptor with USB cable and Mini type connector<br />

• Remote control (optional)<br />

These components are all part of the stand-alone package, which is available as an<br />

option.<br />

Connecting the power supply<br />

1 Insert the mains power adapter into a wall socket.<br />

2 Insert the Mini USB connector on the<br />

mains power adaptor’s connection cable<br />

into the small port on the bottom left of<br />

the back of the camera. Make sure that<br />

the connector is aligned correctly, the<br />

wider edge must be at the top!<br />

The LED on the top lights up red for around<br />

ten seconds while the camera is initializing.<br />

As soon as the LED turns green, the camera<br />

is ready.<br />

Inserting memory card for operation without PC<br />

1 New memory cards must first be<br />

formatted on a PC. Select the FAT32<br />

filesystem format.<br />

2 Slide the SD or SDHC memory card<br />

gently into the horizontal card slot with<br />

the gold contacts to the front and<br />

pointing downwards. As soon as it<br />

clicks, the card is in place.<br />

If you later wish to remove the memory<br />

card, press it further into the card slot and it<br />

will then spring out again automatically.<br />

Acquiring your first image<br />

1 Switch on the microscope’s illumination again.<br />

2 Place the desired object onto the slide.<br />

3 Focus on a color-neutral structure on the object, in such a way that it fills the<br />

image.<br />

4 On the camera, press the button on the right above the card slot, labeled AWB,<br />

to perform the white balance. This is done within a second and can be used<br />

immediately.<br />

5 Set your desired frame.<br />

6 Press the button labeled with the camera symbol.The camera acquires the image,<br />

the LED flashes green briefly and the image is saved.<br />

7 Remove the memory card by pressing it in further.<br />

8 Insert the memory card into your PC’s SD card reader or the accessory card reader.<br />

9 Start an image processing program of your choice that can read JPEG files, load the<br />

image from the memory card and process your image as you wish.<br />

i<br />

Note:<br />

Activate the optical path for the camera when either using AWB or acquiring images.<br />

If you have problems with the AWB please use a conversion filter to adjust the color<br />

temperature of the illumination to the camera limit.<br />

!<br />

Operation without PC connection


Operation with PC connection and software<br />

Configuring and controlling the camera from<br />

the PC<br />

i<br />

Connecting the power supply and establishing a PC<br />

connection<br />

To control the camera from a PC it must be connected to the PC by means of a<br />

USB cable. The PC also provides the power supply for the camera via this cable,<br />

meaning that there is no need for a separate power supply.<br />

!<br />

Caution!<br />

Serious damage to the camera as a result of power spikes and electrostatics! When<br />

you connect the PC and camera, serious damage can be caused to the camera.<br />

In order to avoid this, please observe the following sequence when inserting the<br />

connectors!<br />

!<br />

1 Switch off the PC in order to prevent damage caused by power spikes and<br />

electrostatics.<br />

2 Check the USB cable for any signs of external damage.<br />

3 Plug the flat USB connector into a free USB port on the PC.<br />

4 Insert the Mini USB connector into the small port on the bottom left of the<br />

back of the camera. Make sure that the connector is aligned correctly, the<br />

wider edge must be at the top!<br />

5 Now switch the PC back on.<br />

If you later wish to disconnect the PC and camera again, proceed as follows<br />

(always hold the connector while pulling, not the cable!):<br />

6 First disconnect the connector from the camera housing.<br />

7 Then pull the connector out of the PC port.<br />

Installing configuration program and AxioVision LE<br />

A DVD with a setup program is supplied. The setup gives you the option of<br />

installing the configuration program for the camera, the free imaging software<br />

AxioVision LE or both together.<br />

To do so, follow the instructions in the dialog boxes on the screen.<br />

When you perform the setup, the camera driver will also be installed at<br />

the same time. In addition, when AxioVision LE is installed a check will be<br />

performed to see whether an older version of the program already exists. If it<br />

does, this will be updated.<br />

A manual and online help for AxioVision LE can also be found on the DVD.<br />

Configuring the camera<br />

It is possible to configure the camera from the PC using the configuration<br />

program. This allows a range of processes that take place in the camera to<br />

be preset in detail. These settings are then saved onto the camera from the<br />

configuration program.<br />

With the help of the configuration program it is possible for the stand-alone<br />

operation of the AxioCam <strong>ERc</strong> <strong>5s</strong> to be individually configured. This means<br />

that, using this configuration program, you can change various settings for<br />

the images that you save directly onto the SD card when the button on the<br />

camera is pressed. When the program is closed the parameters that have been<br />

changed can be transferred onto the camera as presets (user settings) and used<br />

automatically for your next images.<br />

If the camera is used in stand-alone mode, these settings determine how the<br />

camera stores the photos onto the memory card, even without a PC connection.<br />

Operation with PC connection and software


Configuration program<br />

Basic functions and settings<br />

The configuration program offers numerous functions for processing<br />

photomicrographs and for configuring camera ports. All changes made in the<br />

configuration program are immediately visible in the live image in the large<br />

field on the right.<br />

The basic settings have been optimized in such a way for your first photographs<br />

that most photos should already be usable.<br />

The most important basic functions are explained below.<br />

2<br />

3<br />

4 1<br />

Online help using F1 function key<br />

All functions are described in detail in the online help.<br />

[1] User settings<br />

Saving of several individual presettings. These can be retrieved at any time, e.g.<br />

for different employees/specimens.<br />

[2] Automatic adjustment of the exposure time<br />

Deactivation of the automatic adjustment of the exposure time. The value used<br />

most recently is used as a basic setting. This is helpful for very dark or bright<br />

objects, e.g. in the case of darkfield images.<br />

[3] Saving and closing<br />

This button gives you the option of transferring the settings currently specified<br />

on the property pages to the camera’s internal memory and then closes the<br />

program.<br />

[4] White balance<br />

Balance for a neutral color display that is close to reality (see also Definitions<br />

on the cover!) To achieve this, set a color-neutral scene without a specimen<br />

under the microscope. This should be illuminated as homogeneously as possible.<br />

It should not be too bright and should on no account be a pure white tone!<br />

Auto: Starts the measurement. This method only works if the object has colorneutral<br />

gray or white surfaces.<br />

Interactive: Allows you to define an individual reference point for the white<br />

balance using the mouse pointer. Click on a color-neutral gray/white area.<br />

3200 K: Basic setting for white balance. This setting has been optimized for a<br />

light temperature of halogen light at 3200 K, which leads to image reproduction<br />

that is closest to the impression of the image in the microscope’s eyepiece.<br />

Configuration program


Configuration program (continued)<br />

“Extras” property page<br />

Sharpness<br />

Emphasizes the object’s contrast edges.<br />

A slight enhancement of sharpness<br />

has been preset. This compensates for<br />

the lack of sharpness that occurs in all<br />

digital cameras due to the principles<br />

behind their operation. Excessive<br />

sharpness can, however, make the<br />

image look unnatural!<br />

Review Time<br />

Defines the duration for which moving<br />

images or object movements are<br />

displayed. This varies between 1, 2, 5 or<br />

10 seconds.<br />

Calibration<br />

Improves the image quality of acquired images by correcting defective sensor<br />

pixels and defining the black reference. The camera’s calibration process takes<br />

several minutes.<br />

Note:<br />

i<br />

When you perform this function, the camera should already have been switched on<br />

for at least fifteen minutes. The camera must see an image that is completely black.<br />

To achieve this, close the light path to the camera on the microscope. It is better to<br />

detach the camera from the microscope for this calibration process and to cover the<br />

sensor opening so that it is impermeable to light.<br />

!<br />

“Saving” property page<br />

!<br />

Naming<br />

Enter a prefix into the text field that will<br />

appear in front of the image number in<br />

the file name on the memory card.<br />

Resolution<br />

Determines the image resolution, file<br />

size and quality with which the detail is<br />

reproduced.<br />

Image Compression<br />

The higher the quality, the more space<br />

the image takes up on the memory card.<br />

“Live Image” property page<br />

An external monitor can be connected to the AxioCam <strong>ERc</strong> <strong>5s</strong> to enable coobservation.<br />

This can either take place via the analog S-Video interface or the<br />

digital HDMI interface.<br />

The HDMI signals can be displayed on a standard PC monitor as a DVI signal<br />

via the video adapter, which is available as an option.<br />

i<br />

Note:<br />

It is not possible to display an HD signal (1080p/i or 720p/i) via the digital<br />

interface. Furthermore, only one of the interfaces (analog or digital) can be used.<br />

Co-observation in parallel to the PC is, however, possible and the camera can be<br />

controlled via the software. The settings in the configuration program also have a<br />

direct influence here on the display on the connected monitor.<br />

!<br />

Configuration program (continued)


Configuration program (continued)<br />

Select the desired interface and the format:<br />

HDMI SVGA: display via HDMI port, resolution 800 x 600 pixels<br />

HDMI VGA: display via HDMI port, resolution 640 x 480 pixels<br />

S-Video PAL: display via analog interface in European television format<br />

S-Video NTSC: display via analog interface in US television format<br />

“General Settings” property page<br />

Reload Factory Defaults<br />

Resets camera to a defined status.<br />

i<br />

Note:<br />

Your individual settings will be lost during<br />

this process!<br />

!<br />

Time And Date<br />

Setting Factory default<br />

Autom. exposure activated<br />

Sharpness level 0<br />

Image preview 5 s<br />

Prefix<br />

image<br />

Resolution 2560 x 1920<br />

!<br />

Synchronizes camera with the date and<br />

time indicated on your PC.<br />

For your notes<br />

Configuration program (continued)


AxioVision software<br />

Software products<br />

AxioVision (LE) is the free imaging software available from <strong>Carl</strong> <strong>Zeiss</strong>.<br />

The program is installed in the manner described in the section “Installing<br />

configuration program and AxioVision LE” on the “Operation with PC<br />

connection and software” tab.<br />

AxioVision (full version) is the standard software available from <strong>Carl</strong> <strong>Zeiss</strong><br />

for image acquisition, analysis, processing and archiving.<br />

Acquiring your first image<br />

The steps below describe the process for acquiring a simple image. You can<br />

access the online help for each function using the F1 function key.<br />

1 Start the AxioVision software (LE or full version).<br />

2 If you have several cameras connected to the system, select the desired<br />

camera in the Acquisition menu | Select Camera.<br />

3 Switch the microscope’s light path to the phototube.<br />

4 In order to display the camera’s live image, click on the Live button (or the<br />

F3 key).<br />

5 To adjust the camera and the live image display, click on the Properties<br />

button. Enter the desired settings on the Display, Adjust, Frame and<br />

General property pages.<br />

––<br />

Set the exposure time: Adjust property page, Measure button<br />

––<br />

Focus the camera image<br />

––<br />

Select the frame<br />

––<br />

Select the appropriate scaling for the objective: Pixel drop-down list<br />

6 Acquire a single image using the Snap button.<br />

7 To adjust the image subsequently, click on the Properties button and then<br />

on the Display property page.<br />

––<br />

Adjust the image individually: Brightness and Contrast sliders<br />

––<br />

Set brightness and contrast automatically: Min/Max button<br />

––<br />

Undo changes: Linear button<br />

––<br />

Show scale bars in the image: Scale bar button<br />

––<br />

Generating annotations: Draw in annotations button<br />

––<br />

Adjust live image size: Navigator button<br />

8 Save the image (ZVI image format) using the Save button or export the<br />

image to an image format of your choice via the File menu | Export.<br />

i<br />

Note:<br />

The AxioCam <strong>ERc</strong> <strong>5s</strong> differs from other AxioCam cameras from <strong>Carl</strong> <strong>Zeiss</strong> on account<br />

of a number of functions. For example, the AxioCam <strong>ERc</strong> <strong>5s</strong> already works with a<br />

color profile in the live image. This means that it is no longer necessary to perform<br />

an adjustment to the gamma value of the monitor.<br />

!<br />

Additional software modules<br />

Amongst the wide range of AxioVision software modules (LE and full version),<br />

the following are particularly useful for the AxioCam <strong>ERc</strong> <strong>5s</strong>:<br />

!<br />

Module<br />

HDR Imaging<br />

Interactive<br />

Measurement<br />

Description<br />

Extends the camera’s dynamic range. By acquiring a series of<br />

exposures and merging the individual images to form one image it is<br />

possible to achieve finer brightness gradations in the resulting image<br />

and a wider contrast range.<br />

Collection of measurement tools for analyzing images directly within<br />

AxioVision. The results are displayed in tabular form or can be<br />

embedded in special report templates.<br />

AxioVision software


Troubleshooting<br />

Problem Cause Solution<br />

LED not<br />

illuminating<br />

LED flashing red<br />

LED illuminated<br />

red<br />

Acquisition and<br />

AWB buttons do<br />

not respond<br />

Firmware update<br />

does not work<br />

No live image<br />

of the camera<br />

is visible in<br />

AxioVision<br />

Newly acquired<br />

photos are not<br />

visible on the<br />

memory card<br />

in Microsoft ®<br />

Windows<br />

Explorer<br />

The file names<br />

for new photos<br />

are counting up<br />

from 1 again<br />

The camera<br />

“forgets” the<br />

settings<br />

The camera is not<br />

receiving power via<br />

the USB cable<br />

USB cable is not<br />

connected to a<br />

suitable power<br />

adapter<br />

Unsuitable USB cable<br />

The camera is<br />

indicating an error<br />

The camera is not<br />

ready for operation<br />

The buttons are<br />

disabled while<br />

AxioVision is open<br />

An SD card must<br />

be inserted for an<br />

update to take place<br />

The camera is not<br />

recognized by<br />

AxioVision<br />

Microsoft ® Windows<br />

Explorer has not<br />

updated the content<br />

of the card<br />

The internal counter<br />

in the camera has<br />

been set to 1<br />

The battery inside<br />

the camera is empty<br />

Connect the camera to a PC that is running<br />

or to the plugged-in mains power adapter via<br />

the USB port.<br />

Requirements: output of at least 5 V DC at no<br />

less than 500 mA.<br />

Use the original USB cable supplied by <strong>Carl</strong><br />

<strong>Zeiss</strong>.<br />

SD card is full: insert new card or delete<br />

images from the card being used.<br />

SD card is write-protected: slide the tab on<br />

the card to the “unlocked” position.<br />

SD card is defective or not formatted: format<br />

card; replace if error is not resolved.<br />

Wait until initialization is completed (approx.<br />

ten seconds).<br />

Disconnect USB cable for ten seconds and<br />

then re-connect; wait until initialization is<br />

completed.<br />

Activate acquisition from AxioVision or close<br />

AxioVision.<br />

Insert a formatted, unprotected SD card with<br />

at least 10 MB of free memory space.<br />

Several cameras have been connected to<br />

the PC: select the AxioCam <strong>ERc</strong> <strong>5s</strong> from the<br />

camera selection menu.<br />

The configuration program may have been<br />

opened in parallel: close both programs and<br />

then reopen AxioVision only.<br />

Take the memory card out of the camera,<br />

wait for three seconds, then reinsert it;<br />

Microsoft ® Windows Explorer will now reread<br />

the content of the card.<br />

Check the setting in the configuration<br />

program!<br />

Note: each time the memory card is<br />

formatted the counter is reset to 1.<br />

The camera contains a lithium battery with<br />

a service life of around five years. After this<br />

time it must be replaced by the service team.<br />

Troubleshooting


Care / Maintenance / Disposal<br />

Care<br />

Cleaning the camera housing<br />

You can clean the camera housing from time to time using a dry or slightly<br />

dampened, lint-free cloth. The housing is not water-tight, however. Do not use<br />

harsh cleaning agents or solvents.<br />

Cleaning the filter glass<br />

Only use suitable agents for cleaning the filter glass! Dust on the filter glass<br />

can be removed using an optical brush, blower brush, cotton swab, optical<br />

paper or a lint-free cotton cloth. Please refer to the brochure “The Clean<br />

Microscope” (www.zeiss.de/mikro - enter “The Clean Microscope” into the<br />

search field) for further information.<br />

Maintenance<br />

Never open the camera housing! There are no components or control or<br />

maintenance elements on the camera housing or inside the camera that the<br />

user would be able to repair him/herself. If you are unable to resolve a problem<br />

using the troubleshooting table on the back of this Infoflip, please contact the<br />

support team! You will find the support address at www.zeiss.de/axiocam.<br />

Disposal<br />

The product and its accessories comply with EU directives 2002/95/EC (RoHS)<br />

and 2002/96/EC (WEEE).<br />

We have implemented a take-back and recycling process that ensures that<br />

proper recycling is carried out in accordance with the aforementioned EU<br />

directives.<br />

Please contact your <strong>Carl</strong> <strong>Zeiss</strong> sales/service organization for details relating to<br />

disposal and recycling.<br />

This product must not be disposed of with domestic waste or using municipal<br />

waste disposal services. In the event of resale, the seller must inform the buyer<br />

of the need to dispose of the product appropriately.<br />

For your notes<br />

Care / Maintenance / Disposal


Marketer and support:<br />

<strong>Carl</strong> <strong>Zeiss</strong> MicroImaging GmbH<br />

07740 Jena, Germany<br />

BioSciences | Göttingen Location<br />

Tel.: +49 551 5060 660<br />

Fax: +49 551 5060 646<br />

E-mail: micro@zeiss.de<br />

www.zeiss.de/axiocam<br />

M 60-3-0034 e - printed 09.2010<br />

Infoflip is a registered trademark. This Infoflip is Made in Germany by Infoflip Ulm e. K. IF.G.13.00617.E.01

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