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Axiocam ERc 5s Operating Instructions - Carl Zeiss

Axiocam ERc 5s Operating Instructions - Carl Zeiss

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Control elements, indicators and connections<br />

1<br />

2 3<br />

4<br />

5<br />

Front of camera:<br />

1 LED indicator<br />

2 Manual acquisition<br />

3 AWB: Automatic<br />

white/gray balance<br />

4 Receiver for IR remote<br />

control<br />

5 Card slot for SD/SDHC<br />

memory cards<br />

1<br />

2<br />

3<br />

Back of camera:<br />

1 USB port for data<br />

transfer and power<br />

supply<br />

2 DVI signal via HDMI<br />

port for monitor, TV<br />

or beamer<br />

3 S-Video connection for<br />

recorder or monitor<br />

Definitions<br />

SD memory card: Digital storage medium for recording image data and<br />

other information. On SD cards an amount of 4 GB of data can be stored;<br />

up to 32 GB on new SDHC cards (HC=high capacity). SD cards should be<br />

formatted on a PC to FAT32 format.<br />

C-mount adapter: The C-mount is a standardized mounting thread<br />

for camera lenses. In microscopy the C-mount is used to connect digital<br />

cameras. So it is guaranteed that the image produced by the microscope<br />

objective is large enough to illuminate the small camera sensors.<br />

AWB (Automatic White Balance): The purpose of the white balance is<br />

to calibrate the camera to the color temperature of the incident light and<br />

thereby prevent color cast. However, the automatic white balance that is<br />

normally used does not work, or does not work sufficiently well, in the case<br />

of technical surfaces or macros. This is because these objects often do not<br />

have any defined pure-white or color-neutral surfaces that the automatic<br />

white balance can take as a basis for the calibration. Instead it uses the<br />

average values of the individual red, green and blue (RGB) color channels<br />

for reference. It is therefore necessary to perform the white balance<br />

manually using defined color-neutral objects.<br />

Parfocality: According to Professor A. Köhler, the focus plane set should<br />

be retained when eyepieces or objectives are changed on the microscope.<br />

Ideally, the object should appear just as sharp as it did before the eyepiece<br />

or objective was changed. Today, this also applies to the phototube for the<br />

acquisition of images using a digital camera: the sharpness of the camera’s<br />

images should correspond to that of the image you see when you look<br />

through the eyepiece. It is possible to set the parfocality of the camera even<br />

with the help of a reticle in the intermediate image of the eyepiece or with<br />

the help of an adapted monitor.

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