Tabletop Rotors and Tubes

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Illustrations Page Figure 1-1. Fixed Angle, Swinging Bucket, Vertical Tube, and Near Vertical Tube Rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2 Figure 1-2. Plunger Mechanism in Locked and Released Positions . . . . . . . . . . . . . 1-3 Figure 1-3. Figure 1-4. Particle Separation in Fixed Angle, Swinging Bucket,Vertical Tube, and Near Vertical Tube Rotors. . . . . . . . . . . . . . . . . . . . . . . . . 1-5 Sedimentation Coefficients (in Svedberg Units) for Some Common Biological Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-11 Figure 1-5. Arranging Tubes Symmetrically in a Rotor . . . . . . . . . . . . . . . . . . . . . 1-14 Figure 3-1. Filling OptiSeal Tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5 Figure 3-2. The Cordless Quick-Seal Tube Topper . . . . . . . . . . . . . . . . . . . . . . . . . 3-7 Figure 4-1. Fixed Angle Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2 Figure 4-2. Swinging Bucket Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6 Figure 4-3. Vertical Tube Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-11 Figure 4-4. Near Vertical Tube Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-12 Figure B-1. Precipitation Curves for the MLN-80 Rotor . . . . . . . . . . . . . . . . . . . . . B-3 Figure B-2. CsCl Gradients at Equilibrium for the MLN-80 Rotor. . . . . . . . . . . . . . B-4 Tabletop Ultracentrifuge Rotors and Tubes vii
Tables Page Table 1-1. Rotors Used in Beckman Coulter Tabletop Ultracentrifuges . . . . . . . . . 1-7 Table 2-1. Characteristics and Chemical Resistances of Tube Materials . . . . . . . . 2-2 Table 3-1. Filling and Capping Requirements for Tubes . . . . . . . . . . . . . . . . . . . . 3-4 Table 3-2. OptiSeal Tubes and Accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5 Table 5-1. Tube Sterilization and Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7 Table C-1. Commonly Used Gradient Materials with Their Solvents . . . . . . . . . . C-2 Table C-2. Table C-3. Density, Refractive Index, and Concentration Data— Cesium Chloride at 25°C, Molecular Weight = 168.37 . . . . . . . . . . C-3 Density, Refractive Index, and Concentration Data—Sucrose at 20°C, Molecular Weight = 342.3 . . . . . . . . . . . . . . . . . . . . . . . . . C-4 Table C-4. Density Conversion for Cesium and Rubidium Salts at 20°C . . . . . . . C-5 viii Tabletop Ultracentrifuge Rotors and Tubes
Page 1 and 2: TLR-IM-9 ROTORS AND TUBES For Beckm
Page 3 and 4: Mechanical Safety • Use only comp
Page 5 and 6: SCOPE OF THIS MANUAL This manual co
Page 7 and 8: Contents Page Cellulose Propionate
Page 9: Contents Page Tube and Accessory Ca
Page 13 and 14: Rotors Fixed Angle Rotor Swinging B
Page 15 and 16: Rotors ! CAUTION In all tabletop ul
Page 17 and 18: Rotors • Near vertical tube rotor
Page 19 and 20: Rotors Table 1-1. Rotors Used in Be
Page 21 and 22: Rotors where s is the sedimentation
Page 23 and 24: Rotors The gradient may be preforme
Page 25 and 26: Rotors ROTOR BALANCE The mass of a
Page 27 and 28: Rotors • Cesium Chloride Gradient
Page 29 and 30: Tubes and Accessories thinwall poly
Page 31 and 32: Tubes and Accessories POLYALLOMER T
Page 33 and 34: Tubes and Accessories organic solve
Page 35 and 36: Tubes and Accessories SPACERS AND F
Page 37 and 38: Using Tubes and Accessories You can
Page 39 and 40: Using Tubes and Accessories Table 3
Page 41 and 42: Using Tubes and Accessories ➠ NOT
Page 43 and 44: Using Tubes and Accessories ! CAUTI
Page 45 and 46: Using Tubes and Accessories METHOD
Page 47 and 48: Using Tubes and Accessories Polycar
Page 49 and 50: Using Tubes and Accessories Spacer
Page 51 and 52: Using Tubes and Accessories QUICK-S
Page 53 and 54: 4 Using Rotors This section contain
Page 55 and 56: Using Rotors ROTOR PREPARATION AND
Page 57 and 58: Using Rotors ! CAUTION In all table
Page 59 and 60: Using Rotors ROTOR PREPARATION AND
Page 61 and 62:
Using Rotors OPERATION For runs at
Page 63 and 64:
Using Rotors VERTICAL TUBE ROTORS D
Page 65 and 66:
Using Rotors Rotor Preparation and
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Using Rotors 3. Refer to the centri
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Care and Maintenance CLEANING Wash
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Care and Maintenance STERILIZATION
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Care and Maintenance TUBE AND ACCES
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Care and Maintenance ! CAUTION Do n
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Care and Maintenance RETURNING A RO
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Care and Maintenance SYMPTOM POSSIB
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Appendix B The Use of Cesium Chlori
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The Use of Cesium Chloride Curves 1
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The Use of Cesium Chloride Curves T
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Appendix C Gradient Materials This
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Gradient Materials Table C-2. Densi
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Gradient Materials Table C-4. Densi
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Glossary of Terms clearing factor,
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Glossary of Terms Radel polyphenyls
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Appendix E References Documents ref
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References DS-640 Formation of Line
Page 101 and 102:
ULTRACENTRIFUGE ROTOR WARRANTY All
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Tabletop Rotors and Tubes

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