Tabletop Rotors and Tubes

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Rotors At Speed At Rest in Rotor At Rest Outside Rotor Fixed Angle Rotors r min r max Pathlength Swinging Bucket Rotors r min r max Pathlength Vertical Tube Rotors r min r max Pathlength Near Vertical Tube Rotors r min r max Pathlength Figure 1-3. Particle Separation in Fixed Angle, Swinging Bucket, Vertical Tube, and Near Vertical Tube Rotors. Dark gray represents pelleted material, light gray is floating components, and bands are indicated by black lines. Tabletop Ultracentrifuge Rotors and Tubes 1-5
Rotors • Near vertical tube rotors are designed for gradient centrifugation when there are components in a sample mixture that do not participate in the gradient. The reduced tube angle of these rotors significantly reduces run times from the more conventional fixed angle rotors, while allowing components that do not band under separation conditions to either pellet to the bottom or float to the top of the tube. Like the vertical tube rotors, near vertical tube rotors use only Quick-Seal and OptiSeal tubes. Table 1-1 lists Beckman Coulter tabletop preparative rotors. PELLETING (DIFFERENTIAL SEPARATION) Pelleting separates particles of different sedimentation coefficients, the largest particles in the sample traveling to the bottom of the tube first. Differential centrifugation is the successive pelleting of particles of decreasing sedimentation velocities, using increasingly higher forces and/or long run times. The relative pelleting efficiency of each rotor is measured by its k factor (clearing factor): k = ln( r max ⁄ r min ) 10 13 ------------------------------------ ω 2 × ----------- 3600 (1) where ω is the angular velocity of the rotor in radians per second (2πRPM/60, or ω = 0.10472 × rpm), r max is the maximum radius, and r min is the minimum radius. After substitution, k = ( 2.533 × 10 11 ) ln( r max ⁄ rmin ) ------------------------------------------------------------------------- rpm 2 (2) This factor can be used in the following equation to estimate the time t (in hours) required for pelleting: k t = - s (3) 1-6 Tabletop Ultracentrifuge Rotors and Tubes
Page 1 and 2: TLR-IM-9 ROTORS AND TUBES For Beckm
Page 3 and 4: Mechanical Safety • Use only comp
Page 5 and 6: SCOPE OF THIS MANUAL This manual co
Page 7 and 8: Contents Page Cellulose Propionate
Page 9 and 10: Contents Page Tube and Accessory Ca
Page 11 and 12: Tables Page Table 1-1. Rotors Used
Page 13 and 14: Rotors Fixed Angle Rotor Swinging B
Page 15: Rotors ! CAUTION In all tabletop ul
Page 19 and 20: Rotors Table 1-1. Rotors Used in Be
Page 21 and 22: Rotors where s is the sedimentation
Page 23 and 24: Rotors The gradient may be preforme
Page 25 and 26: Rotors ROTOR BALANCE The mass of a
Page 27 and 28: Rotors • Cesium Chloride Gradient
Page 29 and 30: Tubes and Accessories thinwall poly
Page 31 and 32: Tubes and Accessories POLYALLOMER T
Page 33 and 34: Tubes and Accessories organic solve
Page 35 and 36: Tubes and Accessories SPACERS AND F
Page 37 and 38: Using Tubes and Accessories You can
Page 39 and 40: Using Tubes and Accessories Table 3
Page 41 and 42: Using Tubes and Accessories ➠ NOT
Page 43 and 44: Using Tubes and Accessories ! CAUTI
Page 45 and 46: Using Tubes and Accessories METHOD
Page 47 and 48: Using Tubes and Accessories Polycar
Page 49 and 50: Using Tubes and Accessories Spacer
Page 51 and 52: Using Tubes and Accessories QUICK-S
Page 53 and 54: 4 Using Rotors This section contain
Page 55 and 56: Using Rotors ROTOR PREPARATION AND
Page 57 and 58: Using Rotors ! CAUTION In all table
Page 59 and 60: Using Rotors ROTOR PREPARATION AND
Page 61 and 62: Using Rotors OPERATION For runs at
Page 63 and 64: Using Rotors VERTICAL TUBE ROTORS D
Page 65 and 66: Using Rotors Rotor Preparation and
Page 67 and 68:
Using Rotors 3. Refer to the centri
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Care and Maintenance CLEANING Wash
Page 71 and 72:
Care and Maintenance STERILIZATION
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Care and Maintenance TUBE AND ACCES
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Care and Maintenance ! CAUTION Do n
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Care and Maintenance RETURNING A RO
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Care and Maintenance SYMPTOM POSSIB
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Appendix B The Use of Cesium Chlori
Page 83 and 84:
The Use of Cesium Chloride Curves 1
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The Use of Cesium Chloride Curves T
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Appendix C Gradient Materials This
Page 89 and 90:
Gradient Materials Table C-2. Densi
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Gradient Materials Table C-4. Densi
Page 93 and 94:
Glossary of Terms clearing factor,
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Glossary of Terms Radel polyphenyls
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Appendix E References Documents ref
Page 99 and 100:
References DS-640 Formation of Line
Page 101 and 102:
ULTRACENTRIFUGE ROTOR WARRANTY All
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