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Tabletop Rotors and Tubes

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1<br />

<strong>Rotors</strong><br />

This section is an introduction to the Beckman Coulter family of<br />

tabletop preparative ultracentrifuge rotors, providing general information<br />

on rotor design, selection, <strong>and</strong> operation. Rotor designs<br />

described are fixed angle, swinging bucket, vertical tube, <strong>and</strong> near<br />

vertical tube type. Specific instructions for using each type of rotor<br />

are contained in Section 4. Care <strong>and</strong> maintenance information for all<br />

of these rotors is contained in Section 5.<br />

GENERAL DESCRIPTION<br />

ROTOR DESIGNATIONS<br />

Beckman Coulter tabletop preparative rotors are named according<br />

to the type of rotor <strong>and</strong> the rotor’s maximum allowable revolutions<br />

per minute (in thous<strong>and</strong>s), referred to as rated speed. For example,<br />

the TLS-55 is a swinging bucket rotor with a maximum speed of<br />

55 000 rpm. Decimal units that are sometimes part of the rotor<br />

name, as in the TLA-120.2 <strong>and</strong> the TLA-120.3, make it possible<br />

to distinguish between different rotors that have the same maximum<br />

allowable speed. An example of each rotor type is shown in<br />

Figure 1-1.<br />

<strong>Tubes</strong> in fixed angle rotors (designated MLA or TLA) are held at an<br />

angle to the axis of rotation in numbered tube cavities. The bodies of<br />

some rotors are fluted to eliminate unnecessary weight <strong>and</strong> minimize<br />

stresses.<br />

In swinging bucket rotors (designated MLS or TLS), containers are<br />

held in rotor buckets attached to the rotor body by hinge pins or a<br />

crossbar. The buckets swing out to a horizontal position as the rotor<br />

accelerates, then seat against the rotor body for support.<br />

<strong>Tabletop</strong> Ultracentrifuge <strong>Rotors</strong> <strong>and</strong> <strong>Tubes</strong><br />

1-1

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