Tabletop Rotors and Tubes

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TLR-IM-9 December 2007 ROTORS AND TUBES For Beckman Coulter Tabletop Preparative Ultracentrifuges Optima MAX, MAX-XP, and MAX-E Optima TLX and TL Series and TL-100 User’s Manual © 2007 Beckman Coulter, Inc. Produced in the U.S.A. CA-1207-PDF
SCOPE OF THIS MANUAL This manual contains general information for properly preparing a rotor for centrifugation in a Beckman Coulter tabletop preparative ultracentrifuge. This manual should be used with the individual rotor instruction manual shipped with each rotor. The rotor manuals provide specific information for each rotor, including special operating procedures and precautions, tube, bottle, and adapter part numbers, and equations to calculate maximum allowable rotor speeds. Each manual has a code number in the upper right-hand corner of the cover page that can be used for reordering. To reorder, contact customer service at 1-800-742-2345 in the United States; outside the U.S., contact your local Beckman Coulter representative. A lot of information is compiled in this manual, and we urge you to read it carefully—especially if this is your first experience with Beckman Coulter products. • In Section 1 you will find descriptions of Beckman Coulter’s currently produced tabletop preparative ultracentrifuge rotors; this should help you determine the appropriate rotor to use for a particular application. Also included in this section is a discussion of rotor materials, components, and centrifugation techniques. • Section 2 describes various tubes, adapters, and spacers to help you choose a particular tube for your application. • Section 3 provides instructions for using tubes and related accessories. • Section 4 contains step-by-step procedures for preparing each type of rotor for a centrifuge run. • Section 5 provides rotor, tube, and accessory care and maintenance information, as well as some diagnostic hints. Please read it. Good rotor care results in longer rotor life. • Several appendixes contain information that may be of special interest: – Appendix A lists chemical resistances for rotor and accessory materials to help determine compatibility with a variety of solutions. – Appendix B describes the use of cesium chloride curves. – Appendix C contains reference information on some commonly used gradient materials. – Appendix D contains a glossary of terms used in this manual. – Appendix E lists references for further reading. Tabletop Ultracentrifuge Rotors and Tubes
Page 1 and 2: TLR-IM-9 ROTORS AND TUBES For Beckm
Page 3: Mechanical Safety • Use only comp
Page 7 and 8: Contents Page Cellulose Propionate
Page 9 and 10: Contents Page Tube and Accessory Ca
Page 11 and 12: Tables Page Table 1-1. Rotors Used
Page 13 and 14: Rotors Fixed Angle Rotor Swinging B
Page 15 and 16: Rotors ! CAUTION In all tabletop ul
Page 17 and 18: Rotors • Near vertical tube rotor
Page 19 and 20: Rotors Table 1-1. Rotors Used in Be
Page 21 and 22: Rotors where s is the sedimentation
Page 23 and 24: Rotors The gradient may be preforme
Page 25 and 26: Rotors ROTOR BALANCE The mass of a
Page 27 and 28: Rotors • Cesium Chloride Gradient
Page 29 and 30: Tubes and Accessories thinwall poly
Page 31 and 32: Tubes and Accessories POLYALLOMER T
Page 33 and 34: Tubes and Accessories organic solve
Page 35 and 36: Tubes and Accessories SPACERS AND F
Page 37 and 38: Using Tubes and Accessories You can
Page 39 and 40: Using Tubes and Accessories Table 3
Page 41 and 42: Using Tubes and Accessories ➠ NOT
Page 43 and 44: Using Tubes and Accessories ! CAUTI
Page 45 and 46: Using Tubes and Accessories METHOD
Page 47 and 48: Using Tubes and Accessories Polycar
Page 49 and 50: Using Tubes and Accessories Spacer
Page 51 and 52: Using Tubes and Accessories QUICK-S
Page 53 and 54: 4 Using Rotors This section contain
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Using Rotors ROTOR PREPARATION AND
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Using Rotors ! CAUTION In all table
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Using Rotors ROTOR PREPARATION AND
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Using Rotors OPERATION For runs at
Page 63 and 64:
Using Rotors VERTICAL TUBE ROTORS D
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Using Rotors Rotor Preparation and
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Using Rotors 3. Refer to the centri
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Care and Maintenance CLEANING Wash
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Care and Maintenance STERILIZATION
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Care and Maintenance TUBE AND ACCES
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Care and Maintenance ! CAUTION Do n
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Care and Maintenance RETURNING A RO
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Care and Maintenance SYMPTOM POSSIB
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Appendix B The Use of Cesium Chlori
Page 83 and 84:
The Use of Cesium Chloride Curves 1
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The Use of Cesium Chloride Curves T
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Appendix C Gradient Materials This
Page 89 and 90:
Gradient Materials Table C-2. Densi
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Gradient Materials Table C-4. Densi
Page 93 and 94:
Glossary of Terms clearing factor,
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Glossary of Terms Radel polyphenyls
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Appendix E References Documents ref
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References DS-640 Formation of Line
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ULTRACENTRIFUGE ROTOR WARRANTY All
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