Tabletop Rotors and Tubes

More documents

Recommendations

Info

Tubes and Accessories Quick-Seal ® Tubes Metal Spacer Dome-Top g-Max Floating Spacer Bell-Top Heat-sealed Quick-Seal tubes are used in swinging bucket, vertical tube, near vertical tube, and in most fixed angle rotors. Single-use Quick-Seal tubes are a convenient form of sealable tube; they are especially useful for the containment of radioactive or pathogenic samples. There are two Quick-Seal tube designs, dome-top and bell-top. • The bell-top simplifies removal of materials that float during centrifugation. • Dome-top tubes hold more volume than their bell-top equivalents. Detailed information about Quick-Seal tubes is contained in publication IN-181. POLYCARBONATE TUBES Polycarbonate is tough, rigid, nonwettable, and glass-like in appearance. Polycarbonate tubes are used in fixed angle rotors, and at least half full in swinging bucket rotors. Speed reduction may be required in some rotors if the tubes are not completely filled. Although polycarbonate tubes may be autoclaved, doing so greatly reduces the usable life of these tubes. Cold sterilization methods are recommended. Washing with alkaline detergents can cause failure. Crazing—the appearance of fine cracks in the tube—is the result of stress “relaxation” and can affect tube performance. These cracks will gradually increase in size and depth, becoming more visible. Tubes should be discarded before cracks become large enough for fluid to escape. These tubes have good tolerance to all gradient media except alkalines (pH greater than 8). They are satisfactory for some weak acids, but are unsatisfactory for all bases, alcohol, and other organic solvents. POLYPROPYLENE TUBES Polypropylene tubes are translucent and are reusable unless deformed during centrifugation or autoclaving. These tubes have good tolerance to gradient media including alkalines. They are satisfactory for many acids, bases, and alcohols, but are marginal to unsatisfactory for most Tabletop Ultracentrifuge Rotors and Tubes 2-5
Tubes and Accessories organic solvents. They can be used with or without caps in fixed angle rotors. Speed reduction is sometimes required with these tubes if run with less than full volume (refer to your rotor manual). POLYETHYLENE TUBES Polyethylene tubes are translucent or transparent and have a good tolerance for use with strong acids and bases. They are reusable but cannot be autoclaved. In swinging bucket rotors, they are used without caps, and with or without caps in fixed angle rotors. ULTRA-CLEAR TUBES Ultra-Clear tubes, made of a tough thermoplastic, are thinwall and not wettable (but can be made wettable; see Section 3). Ultra-Clear tubes are available in two types—open-top and Quick-Seal. They are transparent centrifuge tubes, offering easy location of visible banded samples. Standard straight-wall Ultra-Clear tubes must be filled completely and capped for use in fixed angle rotors. Ultra-Clear tubes are designed to be used one time only. These tubes have good resistance to most weak acids and some weak bases, but are unsatisfactory for DMSO and most organic solvents, including all alcohols. Ultra-Clear tubes should not be autoclaved. CELLULOSE PROPIONATE TUBES Cellulose propionate tubes, used in some fixed angle rotors, are transparent and designed for one-time use. They are used without caps and should be full for centrifuging. They should not be autoclaved or sterilized with alcohol. These tubes have good tolerance to all gradient media including alkalines. They are unsatisfactory for most acids and alcohols. 2-6 Tabletop Ultracentrifuge Rotors and Tubes
Page 1 and 2: TLR-IM-9 ROTORS AND TUBES For Beckm
Page 3 and 4: Mechanical Safety • Use only comp
Page 5 and 6: SCOPE OF THIS MANUAL This manual co
Page 7 and 8: Contents Page Cellulose Propionate
Page 9 and 10: Contents Page Tube and Accessory Ca
Page 11 and 12: Tables Page Table 1-1. Rotors Used
Page 13 and 14: Rotors Fixed Angle Rotor Swinging B
Page 15 and 16: Rotors ! CAUTION In all tabletop ul
Page 17 and 18: Rotors • Near vertical tube rotor
Page 19 and 20: Rotors Table 1-1. Rotors Used in Be
Page 21 and 22: Rotors where s is the sedimentation
Page 23 and 24: Rotors The gradient may be preforme
Page 25 and 26: Rotors ROTOR BALANCE The mass of a
Page 27 and 28: Rotors • Cesium Chloride Gradient
Page 29 and 30: Tubes and Accessories thinwall poly
Page 31: Tubes and Accessories POLYALLOMER T
Page 35 and 36: Tubes and Accessories SPACERS AND F
Page 37 and 38: Using Tubes and Accessories You can
Page 39 and 40: Using Tubes and Accessories Table 3
Page 41 and 42: Using Tubes and Accessories ➠ NOT
Page 43 and 44: Using Tubes and Accessories ! CAUTI
Page 45 and 46: Using Tubes and Accessories METHOD
Page 47 and 48: Using Tubes and Accessories Polycar
Page 49 and 50: Using Tubes and Accessories Spacer
Page 51 and 52: Using Tubes and Accessories QUICK-S
Page 53 and 54: 4 Using Rotors This section contain
Page 55 and 56: Using Rotors ROTOR PREPARATION AND
Page 57 and 58: Using Rotors ! CAUTION In all table
Page 59 and 60: Using Rotors ROTOR PREPARATION AND
Page 61 and 62: Using Rotors OPERATION For runs at
Page 63 and 64: Using Rotors VERTICAL TUBE ROTORS D
Page 65 and 66: Using Rotors Rotor Preparation and
Page 67 and 68: Using Rotors 3. Refer to the centri
Page 69 and 70: Care and Maintenance CLEANING Wash
Page 71 and 72: Care and Maintenance STERILIZATION
Page 73 and 74: Care and Maintenance TUBE AND ACCES
Page 75 and 76: Care and Maintenance ! CAUTION Do n
Page 77 and 78: Care and Maintenance RETURNING A RO
Page 79 and 80: Care and Maintenance SYMPTOM POSSIB
Page 81 and 82: Appendix B The Use of Cesium Chlori
Page 83 and 84:
The Use of Cesium Chloride Curves 1
Page 85 and 86:
The Use of Cesium Chloride Curves T
Page 87 and 88:
Appendix C Gradient Materials This
Page 89 and 90:
Gradient Materials Table C-2. Densi
Page 91 and 92:
Gradient Materials Table C-4. Densi
Page 93 and 94:
Glossary of Terms clearing factor,
Page 95 and 96:
Glossary of Terms Radel polyphenyls
Page 97 and 98:
Appendix E References Documents ref
Page 99 and 100:
References DS-640 Formation of Line
Page 101 and 102:
ULTRACENTRIFUGE ROTOR WARRANTY All
show all

Tabletop Rotors and Tubes

Create successful ePaper yourself

Delete template?

Save as template?