Tabletop Rotors and Tubes

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Using Tubes and Accessories Table 3-2. OptiSeal Tubes and Accessories. Spacers and plugs are shown in the correct orientation for placement onto tubes. Size (mm) Volume (mL) Part Number* (pkg/56) Spacer Rack Assembly Rotor 13 × 33 3.3 361627 361698 (pkg/2) gold aluminum 361650 TLN-100 13 × 48 4.7 361621 Bell-top 361676 (pkg/2) amber Ultem † 361638 TLA-100.4 TLA-110 * Disposable plastic plugs included. † Ultem is a registered trademark of GE Plastics. Stem Base Meniscus Regular Top Stem Base NOTE: Meniscus may not be symmetrical Bell Top Meniscus Between Lines Shown Figure 3-1. Filling OptiSeal Tubes. Stems are large enough to accept standard pipettes. Tabletop Ultracentrifuge Rotors and Tubes 3-5
Using Tubes and Accessories ➠ NOTE If air bubbles occur in the tube shoulder area, tilt and rotate the tube before it is completely filled to wet the tube. Homogeneous solutions of gradients and sample may be loaded into the tubes and centrifuged immediately. (See GRADIENT PREPARATION above.) If the sample is to be layered on top, be sure to allow enough room for the sample so that there is no fluid in the tube stem. 2. After filling the tube, make sure that there is no fluid in the stem. (Draw off excess fluid with a syringe or pipette. If necessary, wipe the inside of the stem with a lintless tissue.) 3. Fill the remaining tubes in the same manner. SEATING THE TUBE PLUGS Eight tubes can be prepared for use at once in the specially designed racks listed in Table 3-2. 1. Make sure that no fluid is in the tube stem and that the stem is clean and dry. 2. Insert a Noryl plug assembly (plug and O-ring—shipped assembled) in each tube stem. 3. Set the plug seating bar on the rack, ensuring that the pegs at each end fit into the rack openings. 4. Press firmly straight down all along the top of the bar. When you remove the bar, the plugs should be straight and seated into the stems. O-ring appears as wide black line No fluid above O-ring 5. Check the tubes to be sure all plugs are seated. If any plugs are not seated, seat them individually. 3-6 Tabletop Ultracentrifuge Rotors and Tubes
Page 1 and 2: TLR-IM-9 ROTORS AND TUBES For Beckm
Page 3 and 4: Mechanical Safety • Use only comp
Page 5 and 6: SCOPE OF THIS MANUAL This manual co
Page 7 and 8: Contents Page Cellulose Propionate
Page 9 and 10: Contents Page Tube and Accessory Ca
Page 11 and 12: Tables Page Table 1-1. Rotors Used
Page 13 and 14: Rotors Fixed Angle Rotor Swinging B
Page 15 and 16: Rotors ! CAUTION In all tabletop ul
Page 17 and 18: Rotors • Near vertical tube rotor
Page 19 and 20: Rotors Table 1-1. Rotors Used in Be
Page 21 and 22: Rotors where s is the sedimentation
Page 23 and 24: Rotors The gradient may be preforme
Page 25 and 26: Rotors ROTOR BALANCE The mass of a
Page 27 and 28: Rotors • Cesium Chloride Gradient
Page 29 and 30: Tubes and Accessories thinwall poly
Page 31 and 32: Tubes and Accessories POLYALLOMER T
Page 33 and 34: Tubes and Accessories organic solve
Page 35 and 36: Tubes and Accessories SPACERS AND F
Page 37 and 38: Using Tubes and Accessories You can
Page 39: Using Tubes and Accessories Table 3
Page 43 and 44: Using Tubes and Accessories ! CAUTI
Page 45 and 46: Using Tubes and Accessories METHOD
Page 47 and 48: Using Tubes and Accessories Polycar
Page 49 and 50: Using Tubes and Accessories Spacer
Page 51 and 52: Using Tubes and Accessories QUICK-S
Page 53 and 54: 4 Using Rotors This section contain
Page 55 and 56: Using Rotors ROTOR PREPARATION AND
Page 57 and 58: Using Rotors ! CAUTION In all table
Page 59 and 60: Using Rotors ROTOR PREPARATION AND
Page 61 and 62: Using Rotors OPERATION For runs at
Page 63 and 64: Using Rotors VERTICAL TUBE ROTORS D
Page 65 and 66: Using Rotors Rotor Preparation and
Page 67 and 68: Using Rotors 3. Refer to the centri
Page 69 and 70: Care and Maintenance CLEANING Wash
Page 71 and 72: Care and Maintenance STERILIZATION
Page 73 and 74: Care and Maintenance TUBE AND ACCES
Page 75 and 76: Care and Maintenance ! CAUTION Do n
Page 77 and 78: Care and Maintenance RETURNING A RO
Page 79 and 80: Care and Maintenance SYMPTOM POSSIB
Page 81 and 82: Appendix B The Use of Cesium Chlori
Page 83 and 84: The Use of Cesium Chloride Curves 1
Page 85 and 86: The Use of Cesium Chloride Curves T
Page 87 and 88: Appendix C Gradient Materials This
Page 89 and 90: Gradient Materials Table C-2. Densi
Page 91 and 92:
Gradient Materials Table C-4. Densi
Page 93 and 94:
Glossary of Terms clearing factor,
Page 95 and 96:
Glossary of Terms Radel polyphenyls
Page 97 and 98:
Appendix E References Documents ref
Page 99 and 100:
References DS-640 Formation of Line
Page 101 and 102:
ULTRACENTRIFUGE ROTOR WARRANTY All
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