Tabletop Rotors and Tubes

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2 Tubes and Accessories This section describes various labware used in Beckman Coulter ML and TL series rotors. General instructions for using containers follow in Section 3. Care and maintenance instructions are in Section 5. General rotor use instructions are in Section 4. The individual rotor manual that comes with each rotor provides specific instructions on the tubes and accessories that can be used in a particular rotor. 1 A table of chemical resistances can be found in Appendix A of this manual. LABWARE SELECTION CRITERIA No single tube design or material meets all application requirements. Labware choice is usually based on a number of factors. • The centrifugation technique to be used, including the rotor in use, volume of sample to be centrifuged, need for sterilization, importance of band visibility, and so forth • Chemical resistance—the nature of the sample and any solvent or gradient media • Temperature and speed considerations • Whether tubes are to be reused Table 2-1 contains an overview of some of the characteristics of tube materials. 1 A complete list of tubes and accessories is provided in the latest edition of the Beckman Coulter Ultracentrifuge Rotors, Tubes & Accessories catalog (BR-8101), available at www.beckmancoulter.com. Tabletop Ultracentrifuge Rotors and Tubes 2-1
Tubes and Accessories thinwall polyallomer Tube or Bottle Type Optical Property Table 2-1. Characteristics and Chemical Resistances of Tube Materials. Refer to Appendix A for information about specific solutions. Puncturable Sliceable Reusable Acids (strong) Acids (dilute or weak) Alcohols (aliphatic) transparent yes yes no S U U M S U U U U U S Aldehydes Bases Esters Hydrocarbons (aliphatic) Hydrocarbons (aromatic and halogenated) Ketones Oxidizing Agents (strong) Salts thickwall polyallomer translucent no no* yes S S S M S M M U M U S Ultra-Clear transparent yes yes no S U U S U U U U U U M polycarbonate transparent no no yes M U U M U U U U U M M polypropylene polyethylene translucent/ transparent transparent/ translucent no no yes S S S M S M S M M M S yes no yes S S S S S S U M M M S cellulose propionate transparent no no* no S U U U U M S S U M S S = satisfactory resistance M = marginal resistance U = unsatisfactory resistance * Polyallomer and cellulose propionate tubes with diameters of 5 to 13 mm may be sliced using the CentriTube Slicer (part number 347960) and appropriate adapter plate. ➠ NOTE This information has been consolidated from a number of sources and is provided only as a guide to the selection of tube or bottle materials. Soak tests at 1 g (at 20°C) established the data for most of the materials; reactions may vary under the stress of centrifugation, or with extended contact or temperature variations. To prevent failure and loss of valuable sample, ALWAYS TEST SOLU- TIONS UNDER OPERATING CONDITIONS BEFORE USE. ! WARNING Do not use flammable substances in or near operating centrifuges. 2-2 Tabletop Ultracentrifuge Rotors and Tubes
Page 1 and 2: TLR-IM-9 ROTORS AND TUBES For Beckm
Page 3 and 4: Mechanical Safety • Use only comp
Page 5 and 6: SCOPE OF THIS MANUAL This manual co
Page 7 and 8: Contents Page Cellulose Propionate
Page 9 and 10: Contents Page Tube and Accessory Ca
Page 11 and 12: Tables Page Table 1-1. Rotors Used
Page 13 and 14: Rotors Fixed Angle Rotor Swinging B
Page 15 and 16: Rotors ! CAUTION In all tabletop ul
Page 17 and 18: Rotors • Near vertical tube rotor
Page 19 and 20: Rotors Table 1-1. Rotors Used in Be
Page 21 and 22: Rotors where s is the sedimentation
Page 23 and 24: Rotors The gradient may be preforme
Page 25 and 26: Rotors ROTOR BALANCE The mass of a
Page 27: Rotors • Cesium Chloride Gradient
Page 31 and 32: Tubes and Accessories POLYALLOMER T
Page 33 and 34: Tubes and Accessories organic solve
Page 35 and 36: Tubes and Accessories SPACERS AND F
Page 37 and 38: Using Tubes and Accessories You can
Page 39 and 40: Using Tubes and Accessories Table 3
Page 41 and 42: Using Tubes and Accessories ➠ NOT
Page 43 and 44: Using Tubes and Accessories ! CAUTI
Page 45 and 46: Using Tubes and Accessories METHOD
Page 47 and 48: Using Tubes and Accessories Polycar
Page 49 and 50: Using Tubes and Accessories Spacer
Page 51 and 52: Using Tubes and Accessories QUICK-S
Page 53 and 54: 4 Using Rotors This section contain
Page 55 and 56: Using Rotors ROTOR PREPARATION AND
Page 57 and 58: Using Rotors ! CAUTION In all table
Page 59 and 60: Using Rotors ROTOR PREPARATION AND
Page 61 and 62: Using Rotors OPERATION For runs at
Page 63 and 64: Using Rotors VERTICAL TUBE ROTORS D
Page 65 and 66: Using Rotors Rotor Preparation and
Page 67 and 68: Using Rotors 3. Refer to the centri
Page 69 and 70: Care and Maintenance CLEANING Wash
Page 71 and 72: Care and Maintenance STERILIZATION
Page 73 and 74: Care and Maintenance TUBE AND ACCES
Page 75 and 76: Care and Maintenance ! CAUTION Do n
Page 77 and 78: Care and Maintenance RETURNING A RO
Page 79 and 80:
Care and Maintenance SYMPTOM POSSIB
Page 81 and 82:
Appendix B The Use of Cesium Chlori
Page 83 and 84:
The Use of Cesium Chloride Curves 1
Page 85 and 86:
The Use of Cesium Chloride Curves T
Page 87 and 88:
Appendix C Gradient Materials This
Page 89 and 90:
Gradient Materials Table C-2. Densi
Page 91 and 92:
Gradient Materials Table C-4. Densi
Page 93 and 94:
Glossary of Terms clearing factor,
Page 95 and 96:
Glossary of Terms Radel polyphenyls
Page 97 and 98:
Appendix E References Documents ref
Page 99 and 100:
References DS-640 Formation of Line
Page 101 and 102:
ULTRACENTRIFUGE ROTOR WARRANTY All
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Tabletop Rotors and Tubes

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