Tabletop Rotors and Tubes

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Rotors MATERIALS Beckman Coulter rotors are made from either aluminum or titanium. Titanium rotors are stronger and more chemical resistant than the aluminum rotors. Exterior surfaces of titanium rotors are finished with black polyurethane paint. Aluminum rotors are anodized to protect the metal from corrosion. The anodized coating is a thin, tough layer of aluminum oxide formed electrochemically in the final stages of rotor fabrication. A colored dye may be applied over the oxide for rotor identification. The O-rings or gaskets in rotor assemblies or lids, and in swinging bucket caps, are usually made of Buna N elastomer and maintain atmospheric pressure in the rotor if they are kept clean and lightly coated with silicone vacuum grease. Plug gaskets in vertical tube or near vertical tube rotors are made of Hytrel ® and do not require coating. ROTOR RETENTION A rotor retention mechanism on the ultracentrifuge drive hub secures the rotor during the run. A plunger mechanism in the rotor is used to secure a TL series rotor to the drive hub before the run begins (see Figure 1-2). Engaging the plunger ensures that the rotor does not slip on the hub during initial acceleration and that it remains seated during centrifugation. (The Optima MAX, MAX-XP, and MAX-E ultracentrifuges automatically secure the rotor to the drive shaft without the need for engaging the plunger.) Plunger Locked Plunger Released Figure 1-2. Plunger Mechanism in Locked and Released Positions (Vertical Tube Rotor Shown) Tabletop Ultracentrifuge Rotors and Tubes 1-3
Rotors ! CAUTION In all tabletop ultracentrifuge models except the Optima MAX, MAX-XP, and MAX-E, it is very important to lock the rotor in place before beginning the run to ensure that the rotor remains seated during centrifugation. Failure to lock the rotor in place before beginning the run may result in damage to both rotor and instrument. ROTOR SELECTION Selection of a rotor depends on a variety of conditions, such as sample volume, number of sample components to be separated, particle size, run time, required quality of separation, type of separation, and the centrifuge in use. Fixed angle, swinging bucket, vertical tube, and near vertical tube rotors are designed to provide optimal separations for a variety of sample types. Refer to Section 4 for specific information about the use of each type of rotor. • Fixed angle rotors are general-purpose rotors that are especially useful for pelleting subcellular particles and in short-column banding of viruses and subcellular organelles. Tubes are held at an angle (usually 20 to 45 degrees) to the axis of rotation in numbered tube cavities. The tube angle shortens the particle pathlength (see Figure 1-3), compared to swinging bucket rotors, resulting in reduced run times. • Swinging bucket rotors are used for pelleting, isopycnic studies (separation as a function of density), and rate zonal studies (separation as a function of sedimentation coefficient). Swinging bucket rotors are best applied for rate zonal studies in which maximum resolution of sample zones are needed, or pelleting runs where it is desirable for the pellet to be in the exact center of the tube bottom. Gradients of all shapes and steepness can be used. • Vertical tube rotors hold tubes parallel to the axis of rotation; therefore, bands separate across the diameter of the tube rather than down the length of the tube (see Figure 1-3). Vertical tube rotors are useful for isopycnic and, in some cases, rate zonal separations when run time reduction is important. Only Quick-Seal ® and OptiSeal tubes are used in vertical tube rotors, making tube caps unnecessary. 1-4 Tabletop Ultracentrifuge Rotors and Tubes
Page 1 and 2: TLR-IM-9 ROTORS AND TUBES For Beckm
Page 3 and 4: Mechanical Safety • Use only comp
Page 5 and 6: SCOPE OF THIS MANUAL This manual co
Page 7 and 8: Contents Page Cellulose Propionate
Page 9 and 10: Contents Page Tube and Accessory Ca
Page 11 and 12: Tables Page Table 1-1. Rotors Used
Page 13: Rotors Fixed Angle Rotor Swinging B
Page 17 and 18: Rotors • Near vertical tube rotor
Page 19 and 20: Rotors Table 1-1. Rotors Used in Be
Page 21 and 22: Rotors where s is the sedimentation
Page 23 and 24: Rotors The gradient may be preforme
Page 25 and 26: Rotors ROTOR BALANCE The mass of a
Page 27 and 28: Rotors • Cesium Chloride Gradient
Page 29 and 30: Tubes and Accessories thinwall poly
Page 31 and 32: Tubes and Accessories POLYALLOMER T
Page 33 and 34: Tubes and Accessories organic solve
Page 35 and 36: Tubes and Accessories SPACERS AND F
Page 37 and 38: Using Tubes and Accessories You can
Page 39 and 40: Using Tubes and Accessories Table 3
Page 41 and 42: Using Tubes and Accessories ➠ NOT
Page 43 and 44: Using Tubes and Accessories ! CAUTI
Page 45 and 46: Using Tubes and Accessories METHOD
Page 47 and 48: Using Tubes and Accessories Polycar
Page 49 and 50: Using Tubes and Accessories Spacer
Page 51 and 52: Using Tubes and Accessories QUICK-S
Page 53 and 54: 4 Using Rotors This section contain
Page 55 and 56: Using Rotors ROTOR PREPARATION AND
Page 57 and 58: Using Rotors ! CAUTION In all table
Page 59 and 60: Using Rotors ROTOR PREPARATION AND
Page 61 and 62: Using Rotors OPERATION For runs at
Page 63 and 64: Using Rotors VERTICAL TUBE ROTORS D
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Using Rotors Rotor Preparation and
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Using Rotors 3. Refer to the centri
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Care and Maintenance CLEANING Wash
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Care and Maintenance STERILIZATION
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Care and Maintenance TUBE AND ACCES
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Care and Maintenance ! CAUTION Do n
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Care and Maintenance RETURNING A RO
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Care and Maintenance SYMPTOM POSSIB
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Appendix B The Use of Cesium Chlori
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The Use of Cesium Chloride Curves 1
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The Use of Cesium Chloride Curves T
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Appendix C Gradient Materials This
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Gradient Materials Table C-2. Densi
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Gradient Materials Table C-4. Densi
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Glossary of Terms clearing factor,
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Glossary of Terms Radel polyphenyls
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Appendix E References Documents ref
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References DS-640 Formation of Line
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ULTRACENTRIFUGE ROTOR WARRANTY All
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