Tabletop Rotors and Tubes

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Rotors Table 1-1. Rotors Used in Beckman Coulter Tabletop Ultracentrifuges (continued) Rotor Profile and Description Max Speed/ RCF/ k factor a Radial Distances (mm) r max r av r min Number of Tubes × Nominal Capacity (largest tube) Rotor Manual Number TLA-55 e Fixed Angle 45° Angle 55 000 186 000 × g 66 55.0 48.0 25.0 12 × 1.5 mL TL-TB-020 55 000 TLS-55 e 90° Angle g 50 Swinging Bucket 259 000 × g 76.5 59.4 42.2 4 × 2.2 mL TL-TB-006 50 000 MLS-50 c 90° Angle g 71 Swinging Bucket 268 000 × g 95.8 71.1 47.5 4 × 5 mL TL-TB-023 TLA-45 f Fixed Angle 45° Angle 45 000 125 000 × g 99 55.0 48.0 25.0 12 × 1.5 mL TL-TB-012 a Maximum speeds are based on a solution density of 1.7 g/mL for all rotors except the MLA-80; solution density for the MLA-80 is 1.2 g/mL. The k factors are calculated for all Beckman Coulter rotors (using the largest-volume tube) as a measure of the rotor’s relative pelleting efficiency, in water, at 20°C. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (rω 2 ) to the standard acceleration of gravity (g) according to the following formula: RCF = rω -------- 2 g where r is the radius in millimeters, ω is the angular velocity in radians per second (2 π RPM /60), and g is the standard acceleration of gravity (9807 mm/s 2 ). After substitution: b Use only in the Optima MAX-XP ultracentrifuge. c Use only in Optima MAX, MAX-XP, or MAX-E ultracentrifuges. d Before these rotors can be used in a TL-100 ultracentrifuge the instrument must be updated with a new drive spindle and updated operating software (modification kit number 360477). Operation of these rotors in an unmodified TL-100 may cause the rotor to stick or slip on the spindle. e This rotor was tested to demonstrate containment of microbiological aerosols under normal operating conditions of the associated Beckman Coulter centrifuge, when used and maintained as instructed. Validation of microbiological containment was done at an independent third-party testing facility (CAMR, Porton Down, UK, or USAMRIID, Ft. Detrick, MD, U.S.A.). Improper use or maintenance may affect seal integrity and thus containment. f No longer manufactured. g At speed. RCF = 1.12 r ⎛RPM ----------- ⎞ 2 ⎝1000⎠ Tabletop Ultracentrifuge Rotors and Tubes 1-9
Rotors where s is the sedimentation coefficient 1 of the particle of interest in Svedberg units. (Because s values in seconds are such small numbers, they are generally expressed in Svedberg units (S), where 1 S is equal to 10 -13 seconds). It is usual practice to use the standard sedimentation coefficient s 20,ω based on sedimentation in water at 20°C. Clearing factors can be calculated at speeds other than maximum rated speed by use of the following formula: k adj = rated speed of rotor k ⎛----------------------------------------------- ⎞ 2 ⎝ actual run speed ⎠ (4) Run times can also be calculated from data established in prior experiments when the k factor of the previous rotor is known. For any two rotors, a and b: t ----- a t b = k ------ a k b (5) where the k factors have been adjusted for the actual run speed used. Figure 1-4 lists sedimentation coefficients for some common biological materials. The k factors at maximum speeds for Beckman Coulter preparative rotors are provided in Table 1-1. Run times can be shortened in some rotors by using the g-Max system. The short pathlength means less distance for particles to travel in the portion of the tube experiencing greatest centrifugal force, and hence shortened run times. Run times can also be shortened in some rotors by using partially filled thickwall polyallomer and polycarbonate tubes. The k factors for half-filled tubes can be calculated by using an approximate r max and r av in k-factor equation (1). ISOPYCNIC SEPARATIONS A sedimentation-equilibrium, or isopycnic, method separates particles on the basis of particle buoyant density. Each component in the sample travels through the gradient until it reaches an equilibrium position. Particle velocity due to differences in density is given in the following expression: v = d 2 ( ρ p – ρ c ) ------------------------------- × g 18μ (6) 1 1 s = dr/dt × 1/w 2 r, where dr/dt is the sedimentation velocity. 1-10 Tabletop Ultracentrifuge Rotors and Tubes
Page 1 and 2: TLR-IM-9 ROTORS AND TUBES For Beckm
Page 3 and 4: Mechanical Safety • Use only comp
Page 5 and 6: SCOPE OF THIS MANUAL This manual co
Page 7 and 8: Contents Page Cellulose Propionate
Page 9 and 10: Contents Page Tube and Accessory Ca
Page 11 and 12: Tables Page Table 1-1. Rotors Used
Page 13 and 14: Rotors Fixed Angle Rotor Swinging B
Page 15 and 16: Rotors ! CAUTION In all tabletop ul
Page 17 and 18: Rotors • Near vertical tube rotor
Page 19: Rotors Table 1-1. Rotors Used in Be
Page 23 and 24: Rotors The gradient may be preforme
Page 25 and 26: Rotors ROTOR BALANCE The mass of a
Page 27 and 28: Rotors • Cesium Chloride Gradient
Page 29 and 30: Tubes and Accessories thinwall poly
Page 31 and 32: Tubes and Accessories POLYALLOMER T
Page 33 and 34: Tubes and Accessories organic solve
Page 35 and 36: Tubes and Accessories SPACERS AND F
Page 37 and 38: Using Tubes and Accessories You can
Page 39 and 40: Using Tubes and Accessories Table 3
Page 41 and 42: Using Tubes and Accessories ➠ NOT
Page 43 and 44: Using Tubes and Accessories ! CAUTI
Page 45 and 46: Using Tubes and Accessories METHOD
Page 47 and 48: Using Tubes and Accessories Polycar
Page 49 and 50: Using Tubes and Accessories Spacer
Page 51 and 52: Using Tubes and Accessories QUICK-S
Page 53 and 54: 4 Using Rotors This section contain
Page 55 and 56: Using Rotors ROTOR PREPARATION AND
Page 57 and 58: Using Rotors ! CAUTION In all table
Page 59 and 60: Using Rotors ROTOR PREPARATION AND
Page 61 and 62: Using Rotors OPERATION For runs at
Page 63 and 64: Using Rotors VERTICAL TUBE ROTORS D
Page 65 and 66: Using Rotors Rotor Preparation and
Page 67 and 68: Using Rotors 3. Refer to the centri
Page 69 and 70: Care and Maintenance CLEANING Wash
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Care and Maintenance STERILIZATION
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Care and Maintenance TUBE AND ACCES
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Care and Maintenance ! CAUTION Do n
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Care and Maintenance RETURNING A RO
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Care and Maintenance SYMPTOM POSSIB
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Appendix B The Use of Cesium Chlori
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The Use of Cesium Chloride Curves 1
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The Use of Cesium Chloride Curves T
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Appendix C Gradient Materials This
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Gradient Materials Table C-2. Densi
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Gradient Materials Table C-4. Densi
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Glossary of Terms clearing factor,
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Glossary of Terms Radel polyphenyls
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Appendix E References Documents ref
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References DS-640 Formation of Line
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ULTRACENTRIFUGE ROTOR WARRANTY All
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