Tabletop Rotors and Tubes

Rotors
where s is the sedimentation coefficient in Svedberg units, S. A more
accurate way to estimate run times in rate zonal studies is to use
the sω 2 t charts, available in Use of the ω 2 t Integrator (publication
DS-528). If the values of s and ω 2 are known, and gradients are either
5 to 20% or 10 to 30% (wt/wt) sucrose, you can use the charts to
calculate the run time, t. Conversely, if the value of ω 2 t is known,
sedimentation coefficients can be estimated from zone positions.
In most cases, when banding two or three components by rate zonal
separation, run times can be considerably reduced by using reduced
fill levels. Tubes are partially filled with gradient, but the sample
volume is not changed (however, gradient capacity will be reduced).
Thickwall tubes should be used when this technique is employed,
since thinwall tubes will collapse if not full.
If swinging bucket rotors are used with preformed shallow gradients
(