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Fill the specimen Dewar with liquid nitrogen before freezing.<br />
Open the specimen Dewar drawer by pulling the drawer away from the machine.<br />
Position the LN 2 filled specimen Dewar firmly against the metal holder and then close<br />
the drawer.<br />
Specimen LN Dewar<br />
Metal holder<br />
When the system is fully cooled (approximately 30 minutes after the internal LN 2<br />
pump starts) and the small LN 2 Dewar is filled, the main screen shows “ready for<br />
freezing” in the top left corner. The freezing process can begin.<br />
5.2.3 Inserting the specimen holder in the loading station<br />
The loading mechanism must be open to insert the three-piece cartridge consisting of<br />
an upper cylinder, carrier plate and lower cylinder.<br />
Open the loading mechanism by rotating it towards you into the front locking position<br />
with the loading lever.<br />
Hold the bottom half of the closure with the right forefinger and turn the top half to a<br />
vertical position with the loading lever.<br />
Front locking<br />
position of the<br />
loading<br />
mechanism<br />
Loading lever<br />
Page 35 <strong>Leica</strong> <strong>EM</strong> <strong>HPM100</strong> Operating Manual Version 12/10
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