Leica EM HPM100

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2. Identification 2.1 Product mark and type designation Leica EM HPM100 UVN High Pressure Freezer: 100-130V / 50-60Hz Leica EM HPM100 EVN High Pressure Freezer: 200-240V / 50-60Hz 2.2 Name and address of the manufacturer Leica Mikrosysteme GmbH Hernalser Hauptstrasse 219 A-1170 Wien Tel.: +43 1 48899-0 Fax: +43 1 48899-350 Web: www.leica-microsystems.com Page 5 Leica EM HPM100 Operating Manual Version 12/10
3. Product description 3.1 Field of application and proper use The Leica EM HPM100 High Pressure Freezer is used for freezing aqueous specimens for examination of the specimens with an electron microscope, a light microscope or other surface-analysis methods, such as atomic force microscopy, secondary ion mass spectrometry or photoelectron spectroscopy. Additional preparation stages may occur between freezing and examination. Specimens are frozen with liquid nitrogen at a temperature of –197 °C under a pressure of 210 kPa. Only liquefied nitrogen can be used as the cryogen. Various specimens such as tissue, cell cultures, cosmetic products, food, suspensions etc. can be processed. The high pressure greatly reduces the volume of gaseous substances in the specimen, so specimens should not contain any gas bubbles, which could reduce pressure transmission and reduce the freezing quality. When hazardous substances (e.g., radioactive, explosive, toxic or human pathogenic substances) are processed the substance-specific safety precautions must be implemented. The Leica EM HPM100 must not be used beyond the limits specified in Technical Data. 3.2 Dimensions and weight (for transport) Dimensions of the shipping container: Depth: 149cm Width: 81cm Height: 146cm Weight of transport system: approx. 510kg For detailed information on the HPM100 dimensions see Appendix I. 3.3 Consumption and emissions Voltage: Current: Power consumption: LN 2 consumption during cool down: LN 2 consumption in operation: LN 2 consumption per freezing process: Ethanol consumption per freezing process: Volumetric capacity of the LN 2 Dewar: Volumetric capacity of the ethanol tank: Nitrogen gas emission: Noise level during pressure build-up: Maximum noise level during the freezing process: 100-130V / 50-60Hz 200-240V / 50-60Hz max. 7A for 230V max. 14A for 115V max. 1200W approx. 20 liters approx. 5 liters per hour 50 ml 2 ml 20 liters 0.5 liters approx. 50m 3 per day approx. 60dB(A) approx. 75dB(A) Page 6 Leica EM HPM100 Operating Manual Version 12/10
Page 1 and 2: Leica EM HPM100 Operating Manual Ve
Page 3 and 4: Leica EM HPM100 Operating Manual Le
Page 5 and 6: Tabel of contents 1. Introduction .
Page 7: 1. Introduction In order to ensure
Page 11 and 12: 3.5.4 Safety measures when working
Page 13 and 14: • Wear a face shield or safety gl
Page 15 and 16: 4. Preparing the system for use 4.1
Page 17 and 18: Remove the screws and remove the wo
Page 19 and 20: Remove the plastic packaging from t
Page 21 and 22: Undo the loading mechanism from the
Page 23 and 24: Unpack the box containing the stere
Page 25 and 26: Remove the eye piece caps. Attach t
Page 27 and 28: Unscrew the back panel of the Leica
Page 29 and 30: 5. Operating instructions 5.1 Intro
Page 31 and 32: If a USB stick is connected to the
Page 33 and 34: Open the front safety cover and pro
Page 35 and 36: Once the operating pressure has bee
Page 37 and 38: The display on the main screen show
Page 39 and 40: Insert the upper and lower cylinder
Page 41 and 42: The loading mechanism is now closed
Page 43 and 44: 5.3 Actions following the freezing
Page 45 and 46: 5.3.3 Separating and sorting specim
Page 47 and 48: Push the lever forward. The specime
Page 49 and 50: 5.3.5.2 Bake out of the system Once
Page 51 and 52: 5.4 Changing settings on the main s
Page 53 and 54: 5.4.3 Changing the temperature of t
Page 55 and 56: The machine is ready to freeze with
Page 57 and 58: 7. Messages during operation 7.1 St
Page 59 and 60:
Numbers show dimensions in millimet
Page 61 and 62:
Minutes LMS Holdings GmbH Tel. +49(
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Leica EM HPM100

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