Christoph Florian Schaller - FU Berlin, FB MI

Christoph Schaller - STORMicroscopy 33
Table 7.3: Standard deviations for tting multiple beads.
For this purpose we proceed as before
by averaging twenty ts respectively
and monitoring the movement
of the mean spot centers of one bead.
The disappointing result is shown in
Figure 7.6, which cleary reveals that
the spot seems to oscillate for more
than 20 nm. Even taking more (checked
for up to 50) frames for the averaged
centers does not allow us to detect a
drift in the original sense. Unfortunately
this observation is independent
of the applied tting algorithm and the
chosen bead, while the oscillations of
Figure 7.6: Movement of one of the beads.
the individual beads do not coincide
(cf. Figure 7.7).
Consequently we cannot achieve agreement of the experimental results with our simulations here,
though we interpret the insucient imaging quality as responsible. This is reasonable as satisfactory
results (i.e. beads that do not move apart from the drift of the whole sample) are available (cf. the
previous Chapter 7.2).
Nonetheless the numerical integration algorithm performs better than the Gaussian mask t in
terms of tting accuracy and assigned intensity.
Figure 7.7: Movement of four of the beads.