Download the PDF copy - Bruker
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FV to slow to probe biological processes<br />
True for most of <strong>the</strong>m<br />
• Examples:<br />
• Diffusion of proteins: 10 -5 -10 -9 cm 2 .s -1 .<br />
• Polymerization of microtubules: sec-min.<br />
• Protein translocation to nucleus (can be probed by fluorescence): 30<br />
min to 1 h.<br />
• Cell migration: µm/sec-µm/h.<br />
• Cell division: 20 min to 3 days.<br />
• Ideally: less than 10 min per AFM image. Impossible with FV or loss of<br />
resolution.<br />
• Need for acceptable resolution to compare AFM and Fluorescence data (for<br />
instance: increasing number of STED publications).<br />
1/22/2013 BRUKER CONFIDENTIAL<br />
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