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Application Note #135 Quantitative imaging of living biological samples by Peak Force QNM Atomic Force Microscopy • A comprehensive review of Peak Force QNM applications on soft biological samples • Authors are Dr. Alex Berquand and Dr. Ben Ohler (Bruker Nano Inc.). • + New App Note with latest developments and examples to be released soon. 1/22/2013 46
Contact information Alexandre Berquand Life Science Applications Scientist Alexandre.Berquand@bruker-nano.com Tel: +49 174 333 94 62 www.bruker.com/en/products/surface-analysis/atomic-force-microscopy.html www.brukerafmprobes.com/ http://nanoscaleworld.bruker-axs.com/ 1/22/2013 BRUKER CONFIDENTIAL 47
Page 1 and 2: Nanomechanical AFM measurements on
Page 3 and 4: What’s behind “cell mechanics
Page 5 and 6: Usual tools to probe cell mechanics
Page 7 and 8: AFM Resolution Compared to other mi
Page 9 and 10: Combining AFM to IOM Compatibility
Page 11 and 12: Combining AFM to fluorescence Autom
Page 13 and 14: Contact theories in AFM Different m
Page 15 and 16: FV/Fluo Applications in Biology CSK
Page 17 and 18: FV to slow to probe biological proc
Page 19 and 20: Need for a new characterization tec
Page 21 and 22: Overview: PeakForce QNM® Basic Pri
Page 23 and 24: Preliminary test on a stiff sample
Page 25 and 26: Preliminary test on a soft sample.
Page 27 and 28: FV/QNM accuracy in Biology Study on
Page 29 and 30: QNM study on live Hacats Effect of
Page 31 and 32: PeakForce QNM: Much more informatio
Page 33 and 34: Different Euk. cells: Diatoms Inter
Page 35 and 36: PeakForce QNM: Easier Imaging Mecha
Page 37 and 38: QNM on Live Bacteria E. Coli K12 Ba
Page 39 and 40: Correlating topography to Force cur
Page 41 and 42: Combining QNM with functionalized p
Page 43 and 44: The Biological Question: Can we map
Page 45: Challenge: Glycocalyx in force meas

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