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Preliminary test on a soft sample. FV/QNM comparison on a cell Line plot Indentation Modify force parameters Baseline correction Multiple line plots Boxcar filter Run History FV [Sneddon fit] = 10.9 MPa QNM (bearing Analysis) [Sneddon fit] = 21.72 MPa QNM (PFC+export) [Sneddon fit] = 18.42 MPa • The 2 QNM results are very close. • The FV result is different from the QNM results but the number of data point is also quite different + it’s hard to perform the analysis on exactly the same area. • How about an analysis on higher scale (Bio experiment) 1/22/2013 26
FV/QNM accuracy in Biology Study on glioblastoma • Isogenic cell line U251 transfected with tumor suppressive factors. PF error (z: 0-250 pN) Elasticity (z: 0-1.2 MPa) Deformation (z: 0-250 nm) • 5-6 min per image. • 128x128 resolution. • Enough to observe contrast on all mechanical channels. 1/22/2013 BRUKER CONFIDENTIAL 27
Page 1 and 2: Nanomechanical AFM measurements on
Page 3 and 4: What’s behind “cell mechanics
Page 5 and 6: Usual tools to probe cell mechanics
Page 7 and 8: AFM Resolution Compared to other mi
Page 9 and 10: Combining AFM to IOM Compatibility
Page 11 and 12: Combining AFM to fluorescence Autom
Page 13 and 14: Contact theories in AFM Different m
Page 15 and 16: FV/Fluo Applications in Biology CSK
Page 17 and 18: FV to slow to probe biological proc
Page 19 and 20: Need for a new characterization tec
Page 21 and 22: Overview: PeakForce QNM® Basic Pri
Page 23 and 24: Preliminary test on a stiff sample
Page 25: Preliminary test on a soft sample.
Page 29 and 30: QNM study on live Hacats Effect of
Page 31 and 32: PeakForce QNM: Much more informatio
Page 33 and 34: Different Euk. cells: Diatoms Inter
Page 35 and 36: PeakForce QNM: Easier Imaging Mecha
Page 37 and 38: QNM on Live Bacteria E. Coli K12 Ba
Page 39 and 40: Correlating topography to Force cur
Page 41 and 42: Combining QNM with functionalized p
Page 43 and 44: The Biological Question: Can we map
Page 45 and 46: Challenge: Glycocalyx in force meas
Page 47: Contact information Alexandre Berqu

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