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Resolution is important for biologists Example: STED 80 70 60 50 40 30 20 10 0 2007-2008 2009-2010 2011-2012 • Number of STED publications in Biology/Medicine research. • AFM offers similar spatial resolution but is much slower. • Need for higher resolution, higher force control and faster imaging to be complementary to fluorescence techniques. 1/22/2013 BRUKER CONFIDENTIAL 18
Need for a new characterization technique Peak Force Tapping and Peak Force QNM PFT is based on ScanAsyst (fully Automated AFM) Works with most standard AFM probes in the standard AFM cantilever holders. Z piezo is driven with sinusoidal waveform (not a triangle as in force-distance curves). Z drive frequency is 1-2 kHz. Z drive amplitude is fixed at typical value of 150 nm (300 nm peak-to-peak) Vertical motion of probe produces force-distance plots as it taps on the sample. Imaging feedback is based on the Peak Force of the force-distance curve. The probe can be calibrated before the experiment so that all the channels are directly quantitative: PFQNM 1/22/2013 BRUKER CONFIDENTIAL 19
Page 1 and 2: Nanomechanical AFM measurements on
Page 3 and 4: What’s behind “cell mechanics
Page 5 and 6: Usual tools to probe cell mechanics
Page 7 and 8: AFM Resolution Compared to other mi
Page 9 and 10: Combining AFM to IOM Compatibility
Page 11 and 12: Combining AFM to fluorescence Autom
Page 13 and 14: Contact theories in AFM Different m
Page 15 and 16: FV/Fluo Applications in Biology CSK
Page 17: FV to slow to probe biological proc
Page 21 and 22: Overview: PeakForce QNM® Basic Pri
Page 23 and 24: Preliminary test on a stiff sample
Page 25 and 26: Preliminary test on a soft sample.
Page 27 and 28: FV/QNM accuracy in Biology Study on
Page 29 and 30: QNM study on live Hacats Effect of
Page 31 and 32: PeakForce QNM: Much more informatio
Page 33 and 34: Different Euk. cells: Diatoms Inter
Page 35 and 36: PeakForce QNM: Easier Imaging Mecha
Page 37 and 38: QNM on Live Bacteria E. Coli K12 Ba
Page 39 and 40: Correlating topography to Force cur
Page 41 and 42: Combining QNM with functionalized p
Page 43 and 44: The Biological Question: Can we map
Page 45 and 46: Challenge: Glycocalyx in force meas
Page 47: Contact information Alexandre Berqu

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