sample preparation - Harvard Apparatus UK

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sample preparation PrepTip • Patented • Protein or peptide purification • Salt removal • Detergent removal • Mass spectrometry • Affinity purification • Glycoprotein and glycopeptide purification • MALDI-TOF • HPLC • High Performance Capillary Electrophoresis (HPCE) Step 1 PrepTip Start with a mixed sample Aspirate the sample into and out of the tip in order to allow binding of the sample to the media U.S. Patent 6,537,502 • Unique patented interior coating matrix means no more plugging of the tip opening • Easy, rapid flow of sample due to the unobstructed tip opening of PrepTip • Different sample binding media to suit many sample types • Effective for small samples (even with volumes less than 1 µl) • No special equipment necessary - uses standard micro-pipette tip format • Suitable for use with single or multi-channel pipetting devices • Virtually ANY sample-binding media can be used in the solid matrix • Fast, simple, easy to use PrepTip is a revolutionary new method for cleaning and concentrating proteins and other biological samples for further instrumental analysis. PrepTip’s unique, patented feature is that the interior walls of the tip are coated with the sample-binding material. This interior coating technology makes PrepTip the only such product on the market, providing both speed and reliability. Since the tip opening is not plugged by the interior coating process, the sample can flow freely through the opening without back pressure. PrepTip can be used to clean or concentrate any biological sample since the interior walls of the tip can be coated with your choice of binding media. Different binding materials are offered by Harvard Apparatus. Due to its unique features, sample preparation with PrepTip is very fast, simple and highly effective. PrepTip is well suited for a range of applications including mass spectrometry, HPLC and automated sample preparation methods. Step 2 Step 3 Done! Wash away any unbound sample components with a suitable washing solution Elute the desired sample components by aspirating a small volume of a suitable solution into and out of the tip Purified sample in about 30 seconds! 12 www.harvardapparatus.com phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732
sample preparation PrepTip Desired sample components bound to PrepTip Mixed sample The unique process used to coat the interior walls of PrepTip allows the sample to flow freely through the tip opening. How PrepTips are Used Mechanism of Binding Type of Functional Groups Bound Sample Solvents Bound from... Elute Bound Compounds with... Types of Tip Coating Available Reverse Phase Extraction Nonpolar groups (e.g. aromatic and alkyl groups) Polar solutions (e.g. aqueous buffers) Less polar solvents (e.g. aqueous buffers) C4, C8, C18, Hydrophobic polymer Sample Preparation for HPLC Using C-18 PrepTip Before After U.S. Patent 6,537,502 Sample Mixture: Aprotinin (MW 6,500) in 100mM phosphate/acetate buffer www.harvardapparatus.com phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 13
Page 1 and 2: 2010/11 molecular Sample Preparatio
Page 3 and 4: introduction The Sample Preparation
Page 5 and 6: sample preparation Technology Selec
Page 7 and 8: Guide to Sample Preparation Product
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Page 11: sample preparation Sample Prep Pipe
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Page 17 and 18: sample preparation Family of Tipcol
Page 19 and 20: sample preparation SpinColumns Sin
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Page 23 and 24: Membrane-Bottom Filter Plates Sampl
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Page 41 and 42: sample preparation CoZap CoZap Pads
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Page 49 and 50: Introduction to Equilibrium Dialysi
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Page 55 and 56: CPK ® atomic models Harvard Appara
Page 57 and 58: CPK ® atomic models Guide to CPK
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individual atoms Carbon Atoms (cont
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individual atoms Halogen Atoms Halo
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individual atoms Nitrogen Atoms (co
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Phosphorus, Sulfur and Metal Atoms
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accessories Accessories & Tools Ord
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Research and Teaching Sets (continu
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Assembled Nucleic Acid Components n
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nucleic acid components Unassembled
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Assembled Amino Acid Models individ
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assembled models Fully Assembled Mo
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