sample preparation - Harvard Apparatus UK
sample preparation - Harvard Apparatus UK
sample preparation - Harvard Apparatus UK
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<strong>sample</strong> <strong>preparation</strong><br />
Macro SpinColumn <br />
Samples from 75 µl to 150 µl<br />
• Easy to use<br />
• Unrivaled selection of column<br />
packing materials<br />
• High <strong>sample</strong> recovery<br />
• Rapid <strong>sample</strong> <strong>preparation</strong> time<br />
• Custom packed columns are<br />
available on request<br />
Macro SpinColumns<br />
Size Exclusion<br />
Chromatography<br />
SpinColumns<br />
Solid Phase<br />
Extraction<br />
SpinColumns<br />
• DNA purification<br />
• Protein purification<br />
• Peptide purification<br />
• Small molecule,<br />
carbohydrate removal<br />
Apply the <strong>sample</strong><br />
to the top of the SpinColumn<br />
Apply the <strong>sample</strong> to the top<br />
of the SpinColumn<br />
• Radiolabel removal<br />
• Nick translation<br />
Step 1<br />
Step 1<br />
• Affinity separation<br />
• Salt removal<br />
• Buffer exchange<br />
Centrifuge for<br />
1 minute at low speed<br />
Step 2<br />
Step 2<br />
Centrifuge for 1 minute at low<br />
speed. Impurities pass<br />
through column and collect in<br />
receiver tube #1.<br />
The purified <strong>sample</strong> collects in<br />
receiver tube while impurities<br />
remain in the column.<br />
Done!<br />
Step 3<br />
Place column into receiver<br />
tube #2. Add elution buffer to<br />
the column to remove bound<br />
components. Centrifuge<br />
briefly to collect purified<br />
<strong>sample</strong>.<br />
Simply place the SpinColumn in a<br />
centrifuge tube and centrifuge the tube<br />
briefly to separate your <strong>sample</strong>. The<br />
column material binds and purifies the<br />
<strong>sample</strong> according to size and shape,<br />
chemical composition, charge or other<br />
physiochemical properties.<br />
Done!<br />
The purified <strong>sample</strong> collects<br />
in receiver tube #2.<br />
20 www.harvardapparatus.com phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732
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