sample preparation - Harvard Apparatus UK

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sample preparation Flow-Thru DIALYZER (Reusable) • Ultra fast dialysis times are possible due to large membrane surface area • Automation ready • Suitable for wide sample volume range • Inert Teflon dialysis chamber – minimal sample loss • Suitable for constant temperature dialysis • Dialysis • Buffer exchange • Salt removal • Detergent removal • On-line dialysis for HPLC • On-line sample concentration • And more… Flow-Thru DIALYZER Dialysis Buffer In Dialysis Buffer Out Dialysis Buffer Chamber Membrane (1.2 in x 3.2 in) Sample Chamber Sample Solution In Sample Solution Out The Flow-Thru DIALYZER is a unique system for the rapid dialysis of sample volumes from 20 µl to 100 ml. It provides a large surface area for Flow-Thru on-line dialysis with minimal sample loss. The entire dialysis unit is made of Teflon, an inert material, and has two separate serpentine channels superimposed on each other and separated by a dialysis membrane. The length of each channel is about 700 mm. Five different chambers are available (20 µl, 75 µl, 150 µl, 300 µl and 600 µl). Chambers of different volumes can also be superimposed on each other for specific applications. With the Flow-Thru DIALYZER more than 90% of salts or small molecules can be dialyzed from a sample in one cycle (about 10 minutes). Cycles can be repeated automatically through the use of continuous flow systems and the entire dialysis unit can be submerged in a water bath for constant temperature dialysis. 32 www.harvardapparatus.com phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732
sample preparation Flow-Thru DIALYZER (Reusable) (continued) Flow-Thru DIALYZER & Membranes Chamber Volume: 20 µl 75 µl 150 µl 300 µl 600 µl Flo-Thru DIALYZERS System with Pump, Clamps and 10k Da membranes Qty. of 1 SP1 74-1307 SP1 74-1308 SP1 74-1309 SP1 74-1301 SP1 74-1310 Flow-Thru DIALYZERS with Clamping System and 10k Da Membranes Qty. of 1 SP1 74-1303 SP1 74-1304 SP1 74-1305 SP1 74-1300 SP1 74-1306 Flow-Thru DIALYZERS Qty. of 2 SP1 74-1400 SP1 74-1401 SP1 74-1402 SP1 74-1403 SP1 74-1404 Flow-Thru DIALYZER MEMBRANES (pack of 10) A. Regenerated Cellulose MEMBRANES: 1k Da MWCO SP1 74-1510 2k Da MWCO SP1 74-1511 5k Da MWCO SP1 74-1512 10k Da MWCO SP1 74-1513 25k Da MWCO SP1 74-1514 50k Da MWCO SP1 74-1515 B. Cellulose Acetate MEMBRANES: 100 Da MWCO SP1 74-1508 500 Da MWCO SP1 74-1500 1k Da MWCO SP1 74-1501 2k Da MWCO SP1 74-1502 5k Da MWCO SP1 74-1503 10k Da MWCO SP1 74-1504 25k Da MWCO SP1 74-1505 50k Da MWCO SP1 74-1506 100k Da MWCO SP1 74-1507 C. Polycarbonate MEMBRANES: 0.01 um SP1 74-1520 0.05 um SP1 74-1521 0.60 um SP1 74-1522 Membranes are supplied either as dry or in 0.05% sodium azide solution. They are ready to use after rinsing with deionized water and buffer. Regenerated Cellulose membranes are more stable in organic solvents, but the MWCO range is not as sharply defined as that of Cellulose Acetate membranes. Cellulose Acetate membranes have a sharp MWCO range. They are intended only for aqueous solutions, and the presence of an organic solvent is not recommended. Polycarbonate membranes are more stable in organic solvents. They are available in four highly controlled pore sizes for a well defined MWCO range. www.harvardapparatus.com phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 33
Page 1 and 2: 2010/11 molecular Sample Preparatio
Page 3 and 4: introduction The Sample Preparation
Page 5 and 6: sample preparation Technology Selec
Page 7 and 8: Guide to Sample Preparation Product
Page 9 and 10: Guide to Sample Preparation Product
Page 11 and 12: sample preparation Sample Prep Pipe
Page 13 and 14: sample preparation PrepTip Desired
Page 15 and 16: sample preparation ProTip • Fast
Page 17 and 18: sample preparation Family of Tipcol
Page 19 and 20: sample preparation SpinColumns Sin
Page 21 and 22: sample preparation SpinColumns •
Page 23 and 24: Membrane-Bottom Filter Plates Sampl
Page 25 and 26: sample preparation Introduction to
Page 27 and 28: DIALYZER (Reusable) (continued) sa
Page 29 and 30: sample preparation Ultra-Fast DIALY
Page 31: sample preparation SpinDIALYZER &
Page 35 and 36: sample preparation Ultra-Micro Disp
Page 37 and 38: sample preparation Fast Macro Dispo
Page 39 and 40: sample preparation Liposome Prepara
Page 41 and 42: sample preparation CoZap CoZap Pads
Page 43 and 44: sample preparation ElectroPrep Sys
Page 45 and 46: ElectroPrep System (continued) sam
Page 47 and 48: sample preparation Fast Flow-Thru D
Page 49 and 50: Introduction to Equilibrium Dialysi
Page 51 and 52: sample preparation Fast Micro-Equil
Page 53 and 54: sample preparation DispoEquilibrium
Page 55 and 56: CPK ® atomic models Harvard Appara
Page 57 and 58: CPK ® atomic models Guide to CPK
Page 59 and 60: CPK ® sets CPK ® Atomic Model Set
Page 61 and 62: Summary of Unassembled Models, Comp
Page 63 and 64: individual atoms Carbon Atoms (cont
Page 65 and 66: individual atoms Halogen Atoms Halo
Page 67 and 68: individual atoms Nitrogen Atoms (co
Page 69 and 70: Phosphorus, Sulfur and Metal Atoms
Page 71 and 72: accessories Accessories & Tools Ord
Page 73 and 74: Research and Teaching Sets (continu
Page 75 and 76: Assembled Nucleic Acid Components n
Page 77 and 78: nucleic acid components Unassembled
Page 79 and 80: Assembled Amino Acid Models individ
Page 81 and 82: assembled models Fully Assembled Mo

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