sample preparation - Harvard Apparatus UK
sample preparation - Harvard Apparatus UK
sample preparation - Harvard Apparatus UK
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<strong>sample</strong> <strong>preparation</strong><br />
Ultra-Micro DispoDIALYZER <br />
Samples from 1 µl to 5 µl (Single use)<br />
• Micro-volume dialysis<br />
• Economical<br />
• Easy to use<br />
• Leak-proof<br />
• Low protein binding<br />
• High <strong>sample</strong> recovery<br />
• Rapid dialysis/purification<br />
Step 1<br />
Ultra-Micro<br />
DispoDIALYZER <br />
Place <strong>sample</strong><br />
into the<br />
DispoDIALYZER<br />
• Exchange of buffers<br />
• Removal of detergents<br />
• Concentration of <strong>sample</strong>s<br />
• HPLC, HPCE<br />
• Purification of proteins, DNA and RNA<br />
• Removal of excess radiolabel<br />
or PCR-primers<br />
• GC, GC-MS, NMR<br />
The 1 µl to 5 µl Ultra-Micro<br />
DispoDIALYZER is a unique disposable<br />
DIALYZER for the dialysis of very small<br />
<strong>sample</strong>s. The entire dialysis unit<br />
installed with different MWCO<br />
membranes (0.025 cm2 surface area) is<br />
small enough so that dialysis can be<br />
carried out in a microcentrifuge tube.<br />
Sample recovery is quick and easy with<br />
almost 100% of the <strong>sample</strong> recovered<br />
after only a short centrifugation step.<br />
Ultra-Micro DispoDIALYZER includes<br />
two 1.5 ml capped microcentrifuge<br />
collection tubes.<br />
Ultra-Micro DispoDIALYZERS – Ordering Information<br />
Step 2<br />
Step 3<br />
Place the<br />
DispoDIALYZER<br />
into a<br />
microcentrifuge<br />
tube containing<br />
dialysis buffer<br />
Place the<br />
DispoDIALYZE<br />
R inverted into a<br />
new tube and<br />
centrifuge for 1<br />
minute at low<br />
speed<br />
Membrane MWCO<br />
(Daltons)<br />
Qty. of 25 Qty. of 50 Qty. of 100<br />
Cellulose Acetate<br />
100 SP1 74-0603 SP1 74-0604 SP1 74-0605<br />
500 SP1 74-0606 SP1 74-0607 SP1 74-0608<br />
Regenerated Cellulose<br />
1,000 SP1 74-0609 SP1 74-0610 SP1 74-0611<br />
2,000 SP1 74-0612 SP1 74-0613 SP1 74-0614<br />
5,000 SP1 74-0615 SP1 74-0616 SP1 74-0617<br />
10,000 SP1 74-0602 SP1 74-0600 SP1 74-0601<br />
25,000 SP1 74-0618 SP1 74-0619 SP1 74-0620<br />
50,000 SP1 74-0621 SP1 74-0622 SP1 74-0623<br />
Done!<br />
www.harvardapparatus.com phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 35