sample preparation - Harvard Apparatus UK
sample preparation - Harvard Apparatus UK
sample preparation - Harvard Apparatus UK
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<strong>sample</strong> <strong>preparation</strong><br />
ProTip <br />
• Fast, simple, easy to use<br />
• Highly effective, non-silica based<br />
hydrophilic polymer material<br />
• Easy, rapid flow of <strong>sample</strong> due to the<br />
unobstructed tip opening of ProTip<br />
• Effective for small <strong>sample</strong>s<br />
(even with volumes less than 1 µl)<br />
• Tip sizes available to suit a<br />
range of <strong>sample</strong> volumes<br />
• No special equipment necessary -<br />
uses standard micro-pipette tips<br />
• Suitable for use with single or<br />
multi-channel pipetting devices<br />
• Protein purification<br />
• Peptide purification<br />
• Salt removal<br />
• Detergent removal<br />
• Mass spectrometry<br />
• MALDI<br />
• High performance liquid<br />
chromatography (HPLC)<br />
• High performance capillary<br />
electrophoresis (HPCE)<br />
• ESI-MS<br />
ProTip, one of <strong>Harvard</strong> <strong>Apparatus</strong>’ latest<br />
patent-pending technologies, is a new<br />
method for cleaning and concentrating<br />
protein <strong>sample</strong>s for further instrumental<br />
analysis. ProTip offers the same easy<br />
<strong>sample</strong> flow of our PrepTip, but contains a<br />
small quantity of a highly effective<br />
hydrophilic polymer material. The binding<br />
capacity of the polymer used in ProTip is 5<br />
to 10 times higher than that of<br />
silica-based materials. This polymer binds<br />
proteins in the presence of organic<br />
solvents but does not bind impurities,<br />
such as salts and detergents, in the<br />
<strong>sample</strong>. After the protein is bound to the<br />
polymer it can easily be eluted in an<br />
aqueous solution, making purification and<br />
concentration of biological <strong>sample</strong>s with<br />
ProTip fast, simple, and highly effective.<br />
Three sizes are offered to meet your<br />
specific application. The Ultra-Micro<br />
ProTip includes one micro pipett.<br />
Step 1<br />
Step 2<br />
Step 3<br />
ProTip <br />
Start with a mixed<br />
<strong>sample</strong><br />
Aspirate the <strong>sample</strong><br />
into and out of the tip<br />
in order to allow<br />
binding of the <strong>sample</strong><br />
to the media<br />
Wash away any<br />
unbound <strong>sample</strong><br />
components with a<br />
suitable washing<br />
solution<br />
Elute the desired<br />
<strong>sample</strong> components<br />
by aspirating a small<br />
volume of a suitable<br />
solution into and out<br />
of the tip<br />
Counts<br />
6000<br />
4000<br />
A: Sample injected directly. B: Sample concentration<br />
using ProTip.<br />
Counts<br />
8000<br />
6000<br />
Done!<br />
Purified <strong>sample</strong> in<br />
about 30 seconds!<br />
4000<br />
2000<br />
2000<br />
5000<br />
15000 20000 25000 30000 35000 40000<br />
5000 10000 15000 20000 25000 30000<br />
10000 35000 40000<br />
Mass (m/2)<br />
Mass (m/2)<br />
Purification of Cytochrome-C in SDS-Tris-Glycine for MALDI-TOF Mass Spectrometry using ProTip<br />
Order # Product<br />
SP1 74-3700 Ultra-Micro ProTip 1 to 10 µl, pkg. of 24<br />
SP1 74-3701 Ultra-Micro ProTip 1 to 10 µl, pkg. of 96<br />
SP1 74-3702 Micro ProTip 10 to 100 µl, pkg. of 24<br />
SP1 74-3703 Micro ProTip 10 to 100 µl, pkg. of 96<br />
SP1 74-3704 Macro ProTip 100 to 500 µl, pkg. of 24<br />
SP1 74-3705 Macro ProTip 100 to 500 µl, pkg. of 96<br />
www.harvardapparatus.com phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 15
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