sample preparation - Harvard Apparatus UK

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sample preparation Multi-Equilibrium DIALYZER Samples from 0.25 ml to 5 ml (Reusable) Plug • Easy to use • Leak-proof • Reproducible • Fast dialysis times • Available for a range of sample sizes • Up to 20 parallel, simultaneous assays • Autoclavable • Low protein binding • High sample recovery • Made of Teflon – totally inert • Protein binding assays • Protein-drug binding assays • Receptor binding assays • Ligand binding assays • Protein-protein interations • Protein-DNA interactions Emptying Stopper The Harvard Apparatus Multi-Equilibrium DIALYZER provides highly standardized equilibrium dialysis conditions for up to 20 parallel assays. The instrument offers outstanding uniformity of: membrane area, sample volume, degree of agitation. The advantages of this system are that up to 20 cells can be used simultaneously for rapid dialysis under standardized conditions. Experiments conducted using the Multi-Equilibrium DIALYZER are extremely reproducible and leak-proof and can be performed at a constant temperature. The dialyzer cells are made of Teflon, an extremely inert material, and will not interfere with the samples. Multiple cell systems are available (5, 10, 15, 20 cells) at various cell volumes (0.2, 1.0, 2.0 and 5.0 ml). The unit can be sterilized by autoclaving and the cells can be filled easily with a filling clamp. Custom systems with alternate chamber sizes, membranes and power supply are available. Order # Product Multi-Equlibrium DIALYZER Systems SP1 74-1800 Complete Multi-Equilibrium DIALYZER System Cell • Ready-to-Use Teflon Macro Dialysis Cells (1 ml) with Large Surface Area and plugs, pkg. of 20 • Macro Spacers, pkg. of 24 • Carriers for 5 Teflon Dialysis Cells, pkg. of 4 • Variable Speed Drive Unit (12 x 12 x 7.5 in) for 20 Cells, and 110V power supply, pkg. of 1 • Power Supply Adapter (110 V) • Stand, pkg. of 1 • Filling Clamp, pkg. of 1 • Emptying Stoppers, pkg. of 20 • Dialysis Membranes, MWCO 10,000 Daltons, pkg. of 200 Multi-Equilibrium DIALYZER Individual Components SP1 74-1906 Macro Teflon Dialysis Cells, (1 ml), pkg. of 5 SP1 74-1904 Macro Teflon Dialysis Cells (2 ml), pkg. of 5 SP1 74-1905 Macro Teflon Dialysis Cells (5 ml), pkg. of 5 SP1 74-1909 Macro Spacer, pkg. of 6 SP1 74-1911 Macro Cell Carrier, pkg. of 1 SP1 74-1907 Micro Teflon Dialysis Cells (0.2 ml), pkg. of 5 SP1 74-1908 Micro Spacer, pkg. of 6 SP1 74-1910 Micro Cell Carrier, pkg. of 1 Multi-Equilibrium DIALYZER Individual Components Continued: SP1 74-1912 Power Supply Adapter (110 V) SP1 74-1912A Power Supply Adapter (220 V) SP1 74-1913 Filling Clamp, pkg. of 1 SP1 74-1914 Black Plugs, pkg. of 32 SP1 74-1901 Emptying Stoppers, pkg. of 5 SP1 74-1919 Tank with Fittings (14.5 x 9.5 x 8.25 in), pkg. of 1 Membranes for Multi-Equilibrium DIALYZER SP1 74-2100 MWCO 5,000 Daltons, pkg. of 200 SP1 74-2102 MWCO 10,000 Daltons, pkg. of 200 52 www.harvardapparatus.com phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732
sample preparation DispoEquilibrium DIALYZER Samples from 25 µl to 75 µl (Single use) • Easy to use & disposable • Small sample volumes: 25 µl to 75 µl each chamber • Rapid dialysis due to ultra-thin membrane • High-quality regenerated cellulose membranes with MWCOs of 5,000 and 10,000 Daltons • Leak-proof • Protein and Protein-drug binding assays • Receptor binding assays • Ligand binding assays • Protein-protein and Protein-DNA interactions Harvard Apparatus DispoEquilibrium DIALYZER is a single use product for interaction studies and is currently the only such device on the market. The DispoEquilibrium DIALYZER is leak-proof and provides high sample recovery (almost 100 percent). This system is designed for one-time use with samples such as radiolabeled compounds, avoiding the hassle associated with cleaning the dialyzer after use. Each chamber has a capacity of 25 to 75 µl. The DispoEquilibrium DIALYZER utilizes high-quality regenerated cellulose membranes with MWCO’s of 5,000 or 10,000 Daltons. Sample recovery is very easy through centrifugation or via removal with micropipettes. Includes two caps (one black, one white), two 0.65 ml sample tubes per chamber and two pipette tips for delivery/recovery. DispoEquilibrium DIALYZERS – Ordering Information Membrane MWCO (Daltons) Qty. of 25 Qty. of 50 Qty. of 100 Regenerated Cellulose 1,000 SP1 74-2206 SP1 74-2207 SP1 74-2208 5,000 SP1 74-2204 SP1 74-2200 SP1 74-2201 10,000 SP1 74-2205 SP1 74-2202 SP1 74-2203 25,000 – – SP1 74-2218 50,000 – – SP1 74-2217 Cellulose Acetate 100 SP1 74-2209 – – 500 SP1 74-2212 – – 25,000 – – SP1 74-2210 50,000 – – SP1 74-2211 100,000 – – SP1 74-2219 Other MWCO avilable upon request. www.harvardapparatus.com phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 53
Page 1 and 2: 2010/11 molecular Sample Preparatio
Page 3 and 4: introduction The Sample Preparation
Page 5 and 6: sample preparation Technology Selec
Page 7 and 8: Guide to Sample Preparation Product
Page 9 and 10: Guide to Sample Preparation Product
Page 11 and 12: sample preparation Sample Prep Pipe
Page 13 and 14: sample preparation PrepTip Desired
Page 15 and 16: sample preparation ProTip • Fast
Page 17 and 18: sample preparation Family of Tipcol
Page 19 and 20: sample preparation SpinColumns Sin
Page 21 and 22: sample preparation SpinColumns •
Page 23 and 24: Membrane-Bottom Filter Plates Sampl
Page 25 and 26: sample preparation Introduction to
Page 27 and 28: DIALYZER (Reusable) (continued) sa
Page 29 and 30: sample preparation Ultra-Fast DIALY
Page 31 and 32: sample preparation SpinDIALYZER &
Page 33 and 34: sample preparation Flow-Thru DIALYZ
Page 35 and 36: sample preparation Ultra-Micro Disp
Page 37 and 38: sample preparation Fast Macro Dispo
Page 39 and 40: sample preparation Liposome Prepara
Page 41 and 42: sample preparation CoZap CoZap Pads
Page 43 and 44: sample preparation ElectroPrep Sys
Page 45 and 46: ElectroPrep System (continued) sam
Page 47 and 48: sample preparation Fast Flow-Thru D
Page 49 and 50: Introduction to Equilibrium Dialysi
Page 51: sample preparation Fast Micro-Equil
Page 55 and 56: CPK ® atomic models Harvard Appara
Page 57 and 58: CPK ® atomic models Guide to CPK
Page 59 and 60: CPK ® sets CPK ® Atomic Model Set
Page 61 and 62: Summary of Unassembled Models, Comp
Page 63 and 64: individual atoms Carbon Atoms (cont
Page 65 and 66: individual atoms Halogen Atoms Halo
Page 67 and 68: individual atoms Nitrogen Atoms (co
Page 69 and 70: Phosphorus, Sulfur and Metal Atoms
Page 71 and 72: accessories Accessories & Tools Ord
Page 73 and 74: Research and Teaching Sets (continu
Page 75 and 76: Assembled Nucleic Acid Components n
Page 77 and 78: nucleic acid components Unassembled
Page 79 and 80: Assembled Amino Acid Models individ
Page 81 and 82: assembled models Fully Assembled Mo

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