sample preparation - Harvard Apparatus UK

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sample preparation ElectroPrep System • Faster dialysis times due to movement of charged molecules in the electric field • Re-usable • Available for most sample sizes - large or small • Membranes available with MWCO’s to suit almost any application • Easy to use • Leak proof • Autoclaveable • Low protein binding • High sample recovery • Made of Teflon - totally inert ElectroPrep (SP1 74-1196) with Multiple Link-Chambers (sold separately) U.S. Patent 5,340,449 • Electro-elution from gels and solutions • Electro-dialysis (with an average buffer exchange time of 5 to 10 minutes) • On-line electro-dialysis • Electro-concentration • Selective electro-filtration • Size fractionation • Primer removal • Salt removal • Detergent removal • Dye-Terminator removal • See some examples on the following pages The ElectroPrep system from Harvard Apparatus is an extremely versatile patented sample prep technology. This ElectroPrep system is ideal for the rapid purification of proteins, nucleic acids, carbohydrates and other biomolecules. With a run-time of 5 to 10 minutes, ElectroPrep provides speed and convenience, even at the very low currents (5 to 10 mA) used with this system. The sample chambers are made of Teflon, a completely inert material especially suited for high sample recovery. Membranes of different MWCO (molecular weight cut off), from 100 to 300,000 Daltons, can be used for selective buffer exchange, dialysis, filtration, concentration, fractionation and elution. ElectroPrep DIALYZER can be joined with each other or with one or two link chambers in different combinations (see pages 43 & 44) and membranes (see page 46). Open Caps - lock membranes into position, and, in their simplest format, are used to concentrate or desalt sample as shown in Configuration #1 over the page 44. Link Chamber - available in 25, 50, 100, 250 and 500µl volumes - can be connected to a Dialysis Chamber of greater volume to concentrate dialysed product, or can be joined in series with other link chambers and membranes of different MWCOs for the size-fractionation of different dialysed products Membranes - with different MWCOs to accommodate biological molecules ranging from 100 to 300,00Da ElectroPrep DIALYZER - the major receptacle for sample or dialysed product Key Link-Chamber ElectroPrep DIALYZER Membrane Teflon Cap 42 www.harvardapparatus.com phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732
sample preparation ElectroPrep System Configurations Electroprep System Configurations 1. Decide Application: • Electro-Dialysis - Configuration #1 • Electro-Concentration - Configuration #2 • Electro-Separation - Configuration #2 • Electro-Elution - Configuration #2 • Electro-Filtration - Configuration #3 • Electro-Fractionation - Configuration #4 2. Slect Dialysis Chamber Volume 50 µl to 1500 µl (a Chamber can be connected to an Union for increased volume) 3. Choose suitable size, type and MWCO Dialysis Membranes for desired configurations 4. Connect Dialysis Chamber • with a membrane and open-ended Caps for Desalting or Buffer Exchange (Configuration #1) • with a membrane, Union and a smaller volume Chamber for Electro-Concentration (Configuration #2) • with a membrane, Union and equal volume Chamber for Electro-Separation & Electro-Elution (Configuration #2) • with a membrane and Link Chambers for Electro-Filtration (Configuration #3) • with membranes of different MWCO and multiple Link Chambers for Electro-Fractionation (Configuration #4) Example Configurations Most Basic: To Desalt or Buffer Exchange Two Different Volume Chambers: To Selectively Concentrate Configuration #1: Single Chamber with two matching open-ended Caps Configuration #3: One Chamber connected to a Link Larger Volume Chambers: To Purify and Concentrate or Filter Complex Configuration: for Concentration/Filtration/Separation Configuration #2: Two Chambers connected with an Union Configuration #4: Two Chambers with an Union and two Links www.harvardapparatus.com phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 43
Page 1 and 2: 2010/11 molecular Sample Preparatio
Page 3 and 4: introduction The Sample Preparation
Page 5 and 6: sample preparation Technology Selec
Page 7 and 8: Guide to Sample Preparation Product
Page 9 and 10: Guide to Sample Preparation Product
Page 11 and 12: sample preparation Sample Prep Pipe
Page 13 and 14: sample preparation PrepTip Desired
Page 15 and 16: sample preparation ProTip • Fast
Page 17 and 18: sample preparation Family of Tipcol
Page 19 and 20: sample preparation SpinColumns Sin
Page 21 and 22: sample preparation SpinColumns •
Page 23 and 24: Membrane-Bottom Filter Plates Sampl
Page 25 and 26: sample preparation Introduction to
Page 27 and 28: DIALYZER (Reusable) (continued) sa
Page 29 and 30: sample preparation Ultra-Fast DIALY
Page 31 and 32: sample preparation SpinDIALYZER &
Page 33 and 34: sample preparation Flow-Thru DIALYZ
Page 35 and 36: sample preparation Ultra-Micro Disp
Page 37 and 38: sample preparation Fast Macro Dispo
Page 39 and 40: sample preparation Liposome Prepara
Page 41: sample preparation CoZap CoZap Pads
Page 45 and 46: ElectroPrep System (continued) sam
Page 47 and 48: sample preparation Fast Flow-Thru D
Page 49 and 50: Introduction to Equilibrium Dialysi
Page 51 and 52: sample preparation Fast Micro-Equil
Page 53 and 54: sample preparation DispoEquilibrium
Page 55 and 56: CPK ® atomic models Harvard Appara
Page 57 and 58: CPK ® atomic models Guide to CPK
Page 59 and 60: CPK ® sets CPK ® Atomic Model Set
Page 61 and 62: Summary of Unassembled Models, Comp
Page 63 and 64: individual atoms Carbon Atoms (cont
Page 65 and 66: individual atoms Halogen Atoms Halo
Page 67 and 68: individual atoms Nitrogen Atoms (co
Page 69 and 70: Phosphorus, Sulfur and Metal Atoms
Page 71 and 72: accessories Accessories & Tools Ord
Page 73 and 74: Research and Teaching Sets (continu
Page 75 and 76: Assembled Nucleic Acid Components n
Page 77 and 78: nucleic acid components Unassembled
Page 79 and 80: Assembled Amino Acid Models individ
Page 81 and 82: assembled models Fully Assembled Mo

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