Antibody Engineering Conference, San Diego - MorphoSys

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Library Size and Correctness � Sub-library sizes vary between 5E+8 and 4E+9 clones. � Overall correctness: ~85%. Total library size: 1.3E+11 independent clones with more than hundred billion fully correct human antibodies! Page 22 2011 Antibody Engineering © MorphoSys AG
Library QC by Next Generation Sequencing: Redundancy � 454 sequencing of VH of the unselected library (performed using pan-selective primers recognizing all HCs; kappa and lambda libraries „tagged“) � Proprietary software for data evaluation: More than 151.000 Ylanthia VH sequences analyzed Number of replicates: High sequence diversity: From more than 151.000 sequences 94% were CDR- H3 unique (< 6% sequences found in duplicate). Page 23 2011 Antibody Engineering © MorphoSys AG
Page 1 and 2: A New Antibody Library Concept (AB
Page 3 and 4: AGENDA 1. Features of Ylanthia 2. R
Page 5 and 6: Selection of Top 20 HC and 20 LC Ge
Page 7 and 8: Reduction of Potential Post-Transla
Page 9 and 10: Screening 400 HC/LC Pairs for Bioph
Page 11 and 12: Screening for Fab Phage Display Lev
Page 13 and 14: … Screening for IgG1 Expression L
Page 15 and 16: Combination of all Screening Parame
Page 17 and 18: Ylanthia CDRs: Slonomics Inside Pag
Page 19 and 20: Ylanthia CDR-H3 Design � Lengths
Page 21: AGENDA 1. Features of Ylanthia 2. R
Page 25 and 26: Library Features - Correctness �
Page 27 and 28: Antibody Selections - Snapshot* *be
Page 29 and 30: Biophysical Properties of Selected
Page 31 and 32: Biophysical Properties of Selected
Page 33: www.morphosys.com HuCAL Page 33 201

Antibody Engineering Conference, San Diego - MorphoSys

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