Antibody Engineering Conference, San Diego - MorphoSys

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Library QC by Next Generation Sequencing: CDR-H3 Length Distribution � Analysis of more than 151.000 unselected Ylanthia VH sequences: Unselected CDR-H3 length distribution matches design. Page 24 2011 Antibody Engineering © MorphoSys AG
Library Features – Correctness � QC of final library: � Sequence analysis of VL and corresponding VH of at least 48 clones per sub- library; in total almost 4000 antibodies sequenced and evaluated. Overall library correctness: ~85% failed false TRIM TRIM Slonomics kappa lambda LC HC total sequences 2367 1482 3849 3849 mixed or bad sequence 4.3% 4.4% 4.4% 4.8% seq break before KpnI or XhoI 0% 0% 0% 3.6% analyzed sequences 2265 1416 3681 3523 frameshifts 6.2% 8.8% 7.2% 3.0% mixed in AND after CDR3 0.6% 0.8% 0.7% 2.2% stop mutation (w/o frameshift) 0.3% 0.1% 0.2% 0% multiple inserts 0% 0% 0% 4.0% functional clones 93% 90% 92% 91% undesired CDR3 length 0.6% 0.2% 0.5% 0.2% undesired aa 0.7% 0.5% 0.6% 0.4% correctness 92% 90% 91% 90% Page 25 2011 Antibody Engineering © MorphoSys AG
Page 1 and 2: A New Antibody Library Concept (AB
Page 3 and 4: AGENDA 1. Features of Ylanthia 2. R
Page 5 and 6: Selection of Top 20 HC and 20 LC Ge
Page 7 and 8: Reduction of Potential Post-Transla
Page 9 and 10: Screening 400 HC/LC Pairs for Bioph
Page 11 and 12: Screening for Fab Phage Display Lev
Page 13 and 14: … Screening for IgG1 Expression L
Page 15 and 16: Combination of all Screening Parame
Page 17 and 18: Ylanthia CDRs: Slonomics Inside Pag
Page 19 and 20: Ylanthia CDR-H3 Design � Lengths
Page 21 and 22: AGENDA 1. Features of Ylanthia 2. R
Page 23: Library QC by Next Generation Seque
Page 27 and 28: Antibody Selections - Snapshot* *be
Page 29 and 30: Biophysical Properties of Selected
Page 31 and 32: Biophysical Properties of Selected
Page 33: www.morphosys.com HuCAL Page 33 201

Antibody Engineering Conference, San Diego - MorphoSys

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