Issue-4_April - Asian Journal of Research in Chemistry (AJRC)

Abstract
RESEARCH ARTICLE
Quantification of Quercetin and Rutin in Ashwagandharishta by Validated HPTLC Densitometry
Preeti Tiwari 1 * and Rakesh K. Patel 2 ……………………………………………………………………………………….441
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, India.
2 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research,
Kherva-382711, India
ABSTRACT:
Ashwagandharishta is a polyherbal hydro-alcoholic formulation and is used as immunomodulator to promote the
health and longevity by increasing defence against disease and also known for its usefulness in the treatment of
hypercholesterolemia. A simple, precise and accurate HPTLC method has been established for the determination of
quercetin and rutin in Ashwagandharishta–T and Ashwagandharishta-M prepared by traditional and modern
methods respectively and also in its marketed formulation. The developed HPTLC method was validated in terms of
precision, accuracy, LOD, LOQ and specificity. The amount of quercetin in Ashwagandharishta-T, M and its
marketed formulation was found to be 0.0021, 0.00192 and 0.00197 % w/w respectively while rutin was found to be
0.00469, 0.00441 and 0.00464 % w/w respectively. This is the first report for the quantification of quercetin and
rutin in Ashwagandharishta by HPTLC. Furthermore, no TLC densitometric methods have been reported for the
quantification of quercetin and rutin from Ashwagandharishta.
Modulation of Clonazepam Solubility using Solid Dispersion Technique Improves its Solubility and
Bioavailability.
Hanaa A. Fattah 1 , Hammad M. A. 1 , Nagia El-Megrab 1 , Waleed Barakat 2 , Ahmed Samir 1 ………………………446
1 Department of Pharmaceutics, Faculty of Pharmacy, Zagazig University, Egypt.
2 Department of Pharmacology, Faculty of Pharmacy, Zagazig University, Egypt.
ABSTRACT:
The pharmacologic efficacy of benzodiazepines (as clonazepam, CZP) in controlling seizures is directly associated
with their plasma concentration. An emergent epileptic situation necessitates rapid drug absorption. parentral
administration is not convenient in epileptic emergency and oral bioavailability of CZP is limited by its poor water
solubility.
Solid dispersion formulations were developed by physical mixture and solvent evaporation using CZP with the
carriers polyvinyl pyrrolidone K30 (PVP K30, 1:4) or polyethylene glycol 4000 (PEG 4000, 1:4).
Analysis of the solid dispersions by FTIR, DSC, XRD and dissolution studies showed that CZP was molecularly
dispersed in an amorphous form and indicate increased dissolution rate.
In addition, the oral bioavailability of the formulations was significantly improved reaching therapeutic levels within
30 and 60 min in case of PEG 4000 and PVP K30 respectively following oral administration to mice.
In conclusion; PVP K 30 and PEG 4000 can be utilized to increase the solubility of CZP and enhance its oral
bioavailability.
Use of Activated Carbon of Thevetia peruviana wood for the Adsorption of Acid Violet Dye from Aqueous
Solutions
J. Raffiea Baseri 1 , P.N. Palanisamy 2 and P. Sivakumar 3 ………………………………………………………………..456
1 Department of Chemistry, Al-Ameen Engineering College, Erode, Tamilnadu-638 104, India
2 Department of Chemistry, Kongu Engineering College, Perundurai, Erode, Tamilnadu- 638 052,India
3 Department of Chemistry, Arignar Anna Government Arts College, Namakkal, Tamilnadu- 637 002, India