Issue-4_April - Asian Journal of Research in Chemistry (AJRC)

1. Department of pharmaceutical analysis, Anurag pharmacy college, Ananthagiri(V), Kodad(M), Nalgonda (Dt),
Andhra Pradesh, India.508206
2. D.C.R.M. Pharmacy College, Inkollu, Prakasham Dist, Andhra Pradesh, India.
ABSTRACT:
A simple, reproducible and efficient high performance liquid chromatographic method was developed for
simultaneous determination of Irbesartan and Hydrochlorothiazide in tablets. A Hypersil pack BDS C18 column
250X4.6 mm 5µ particle size in isocratic mode with mobile phase containing acetonitrile: Buffer (sodium acetate
anhydrous) (55:45 v/v ) adjusted to pH 3.5 using ortho phosphoric acid. The flow rate was 1.0 ml/min and effluent
was monitored at 260 nm. The retention time and linearity range for Irbesartan and Hydrochlorothiazide were (2.98,
4.83 min) and (5-200, 1-400 g/ml), respectively. The developed method was found to be accurate, precise and
selective for simultaneous determination of Irbesartan and Hydrochlorothiazide in tablet dosage form.
KEYWORDS: Irbesartan, Hydrochlorothiazide, RP-HPLC, simultaneous determination, Validation.
A Minuscule Study of Global Warming on Acetobacter aceti-Elevated Temperature and Its Impact over the
Industries
L. Krishnasamy and K. Jayanthi……………………………………………………………………………………………..477
Dept. of Biotechnology, Hindustan College of arts and science, Kellambakkam-603 103, Chennai, Tamil Nadu
ABSTRACT:
The aim of this study was to investigate the effect elevated temperature on A.aceti which converts ethanol to acetic
acid in the presence of oxygen. A.aceti was incubated at various temperatures like 37°C, 42°C, and 47°C for
overnight. After exposure, the DNA content in each culture was determined. The results showed that an increase in
temperature like 42°C and 47°C produced a marked decrease in the DNA content when compared to the culture
incubated at 37°C. The expression of gene was confirmed using PCR. The PCR result showed that the gene citrate
synthase of A.aceti was expressed in the culture incubated at 37°C and a significant suppression of the gene citrate
synthase was seen in the culture treated at 42°C and 47°C.Therefore the elevated temperature on A.aceti will
significantly affect the various importance of the organism especially in vinegar production where A.aceti plays a
vital role.
KEYWORDS: A.aceti, elevated temperature, vinegar, citrate synthase gene
Solubility Enhancement of Poorly Water Soluble Drug by Solid Dispersion Technique
Ahmed Samir 1 , Nagia El-Megrab 1 , Hanaa A. Fattah 1 , Waleed Barakat 2 ……………………………………………483
Departments of 1 Pharmaceutics, 2 Pharmacology, Faculty of Pharmacy, Zagazig University, Egypt.
ABSTRACT:
The objective of present was to improve the solubility of Clonazepam (CZP), poorly water soluble drug, by solid
dispersion technique using Polyethylene glycol 6000 (PEG 6000) and Urea (UR) as carriers. The Solid dispersion
was prepared by physical mixing and solvent evaporation method. The interaction of the Clonazepam with PEG
6000 and UR was evaluated by the Fourier transform infrared (FTIR) spectroscopy; Differential scanning
Calorimetry (DSC) and X-ray diffraction patterns (XRD). The results from the FTIR and XRD analyses showed that
Solid dispersion might exist in the amorphous form. A DSC result showed that the sharp melting point was
completely disappeared suggesting that the CZP molecularly dispersed in an amorphous form. Dissolution studies
indicate that dissolution rate was remarkably increased in Solid dispersion as compared to the physical mixture and
drug alone. Also the pharmacological studies of the selected formulations [(1:3 CZP : PEG 6000) and (1:2 CZP :
Urea)] were performed. In conclusion PEG 6000 and UR can be a well utilized to increase the solubility of poorly
water soluble drugs.
KEY WORDS: Clonazepam, solid dispersion, Polyethylene glycol 6000, Urea.