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1 Ato-GFP proteins associate with membrane raft microdomains.<br />

Ato1p-GFP and Ato3p-GFP localise to raft patches.<br />

Ato1p-GFP<br />

Ato3p-GFP<br />

Confocal microscopy<br />

1<br />

2<br />

1<br />

1 2<br />

1 2 3 4 5 6<br />

Ato1p-GFP<br />

Extraction, fractionation and PAGE<br />

2 Ato1p-GFP<br />

localisation to raft<br />

patches is<br />

pH-dependent.<br />

TV<br />

TG<br />

H 2<br />

O<br />

pH 5 pH 8<br />

5 30 50 5 35 60 min<br />

3 Production of Ato-GFP proteins is induced by ammonia.<br />

Řičicová et al., BBA-Biomembranes 1768: 1170-1178 (2007)

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